Search results for "DAS"

showing 10 items of 4164 documents

BAG3 and friends: co-chaperones in selective autophagy during aging and disease.

2011

There is a reciprocal change in the expression of two members of the BAG (Bcl-2-associated athanogen) family, BAG1 and BAG3, during cellular aging and under acute stress ("BAG1-BAG3-switch"). BAG3 was recently described as a mediator of a novel macroautophagy pathway that uses the specificity of heat shock protein 70 (HSP70) to misfolded proteins and also involves other protein partners, such as HSPB8. Also crucial for induction and execution of autophagy are sequestosome-1/p62 (SQSTM1/p62) and LC3, an autophagosome-associated protein. In this novel pathway, BAG3 mediates the targeting and transport of degradation-prone substrates into aggresomes via the microtubule-motor dynein. Interestin…

AgingProteasome Endopeptidase ComplexDyneinBAG3Models BiologicalJUNQ and IPODUbiquitinAutophagyAnimalsDiseaseMolecular BiologyAdaptor Proteins Signal TransducingbiologyAutophagyUbiquitinationSignal transducing adaptor proteinDyneinsCell BiologyAdaptation PhysiologicalCell biologyHsp70DNA-Binding ProteinsAggresomeBiochemistrybiology.proteinMolecular ChaperonesTranscription FactorsAutophagy
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Age-Related Changes of Liver Antioxidant Enzymes and 8-Hydroxy-2-Deoxyguanosine During Fetal–Neonate Transition and Early Rat Development

2000

We have studied the pro-antioxidant status of the rat liver on the last day of gestation and at 1, 15, and 30 days of extrauterine life. Representative variables, such as activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase and concentrations of reduced glutathione and 8-hydroxy-2'-deoxyguanosine, were determined in liver to assess the degree of birth-associated oxidative stress during the fetal-neonatal transition and early development of the rat. Percentages by which liver Cu/ZnSOD activity increased over the basal value of the fetal liver were 54%, 95%, and 127% at neonatal days 1, 15, and 30, respectively. There was a lack of induction in the development profil…

Agingmedicine.medical_specialtyAntioxidantmedicine.medical_treatmentClinical Biochemistrymedicine.disease_causeBiochemistryAntioxidantsSuperoxide dismutasechemistry.chemical_compoundFetusPregnancyInternal medicineGeneticsmedicineAnimalsDeoxyguanosineRats WistarMolecular Biologychemistry.chemical_classificationGlutathione PeroxidaseFetusbiologySuperoxide DismutaseGlutathione peroxidaseDeoxyguanosineCell BiologyGlutathioneCatalaseGlutathioneRatsOxidative StressEndocrinologyAnimals NewbornLiverchemistry8-Hydroxy-2'-DeoxyguanosineCatalasebiology.proteinFemaleOxidative stressDNA DamageIUBMB Life (International Union of Biochemistry and Molecular Biology: Life)
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Physiological changes in glutathione metabolism in foetal and newborn rat liver

1991

Glutathione metabolism was studied in isolated hepatocytes from foetal, newborn and adult rats. The GSH/GSSG ratio decreased 15-20-fold through the foetal-neonatal-adult transition. This was mainly due to an increase in GSSG. All enzyme activities involved in the glutathione redox cycle tend to increase during that transition, but the relative increases in glutathione peroxidase and glutathione S-transferase were 3-5 times those of glutathione reductase or glucose-6-phosphate dehydrogenase. GSH synthesis from methionine as a sulphur source was 6 times lower in foetal than in adult hepatocytes. However, when N-acetylcysteine was used as a sulphur donor to by-pass the cystathionine pathway, t…

Agingmedicine.medical_specialtyGPX1GPX3Glutathione reductaseBiochemistrychemistry.chemical_compoundFetusInternal medicinemedicineAnimalsAmino AcidsMolecular Biologychemistry.chemical_classificationMethioninebiologyGlutathione peroxidaseCystathionine gamma-LyaseRats Inbred StrainsCell BiologyGlutathioneGlutathioneCystathionine beta synthaseRatsEndocrinologyAnimals NewbornLiverBiochemistrychemistryembryonic structuresbiology.proteinResearch ArticleCysteineBiochemical Journal
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Assessment of DNA-protein crosslinks in the course of aging in two mouse strains by use of a modified alkaline filter elution applied to whole tissue…

1999

Abstract Two different mouse strains have been used for determination of age dependence of DNA-protein crosslinks by alkaline filter elution: a long lived laboratory strain, NMRI and an accelerated senescence-prone, short lived strain, SAMP1. Five organs were selected: Brain, kidney, lung, heart and liver. Remarkably in all five organs of short lived SAMP1 mice crosslinks increased significantly with age. In NMRI however only in brain and heart a significant rise in old age has been observed, while in the other organs there was no increase in DNA-protein crosslinking. Appreciable mitotic activity which is lacking in brain and heart could be the reason for this difference. Poor repair in all…

Agingmedicine.medical_specialtyProtein dnaSodium ChlorideBiologyMiceInternal medicinemedicineAnimalsHumansMitosisKidneyLungStrain (chemistry)Life spanElutionProteinsDNACross-Linking Reagentsmedicine.anatomical_structureEndocrinologyBiochemistryFemaleEndopeptidase KHeLa CellsDevelopmental BiologyMechanisms of Ageing and Development
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The atypical cannabinoid O-1602 protects against experimental colitis and inhibits neutrophil recruitment.

2011

Background: Cannabinoids are known to reduce intestinal inflammation. Atypical cannabinoids produce pharmacological effects via unidentified targets. We were interested in whether the atypical cannabinoid O-1602, reportedly an agonist of the putative cannabinoid receptor GPR55, reduces disease severity of dextran sulfate sodium (DSS) and trinitrobenzene sulfonic acid (TNBS)-induced colitis in C57BL/6N and CD1 mice. Methods: DSS (2.5% and 4%) was supplied in drinking water for 1 week while TNBS (4 mg) was applied as a single intrarectal bolus. Results: Both treatments caused severe colitis. Injection of O-1602 (5 mg/kg intraperitoneally) significantly reduced macroscopic and histological col…

AgonistMaleCannabinoid receptormedicine.drug_classColonNeutrophilsmedicine.medical_treatmentPharmacologyMotor ActivityInflammatory bowel diseaseArticleReceptors G-Protein-CoupledReceptor Cannabinoid CB2chemistry.chemical_compoundMiceReceptor Cannabinoid CB1CyclohexanesmedicineImmunology and AllergyAnimalsCannabidiolColitisReceptorReceptors CannabinoidPeroxidaseMice KnockoutAnalysis of VarianceO-1602business.industryCannabinoidsDextran SulfateGastroenterologyResorcinolsmedicine.diseaseColitisMice Inbred C57BLChemotaxis LeukocyteDisease Models AnimalchemistryGPR55Neutrophil InfiltrationTrinitrobenzenesulfonic AcidImmunologylipids (amino acids peptides and proteins)CannabinoidbusinessInflammatory bowel diseases
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CB1 cannabinoid receptor-mediated aggressive behavior

2013

This study examined the role of cannabinoid CB1 receptors (CB1r) in aggressive behavior. Social encounters took place in grouped and isolated mice lacking CB1r (CB1KO) and in wild-type (WT) littermates. Cognitive impulsivity was evaluated in the delayed reinforcement task (DRT). Gene expression analyses of monoaminooxidase-A (MAO-A), catechol-o-methyl-transferase (COMT), 5-hydroxytriptamine transporter (5-HTT) and 5-HT1B serotonergic receptor (5HT1Br) in the median and dorsal raphe nuclei (MnR and DR, respectively) and in the amygdala (AMY) were performed by real time-PCR. Double immunohistochemistry studies evaluated COMT and CB1r co-localization in the raphe nuclei and in the cortical (AC…

AgonistMalemedicine.medical_specialtyCannabinoid receptorTime Factorsmedicine.drug_classmedicine.medical_treatmentPoison controlArachidonic AcidsSerotonergicCatechol O-MethyltransferaseAmygdalaCellular and Molecular NeuroscienceMiceDorsal raphe nucleusReceptor Cannabinoid CB1Internal medicinemedicineAnimalsInterpersonal RelationsMonoamine OxidasePharmacologyCannabinoid Receptor AgonistsMice KnockoutSerotonin Plasma Membrane Transport ProteinsAmygdalaSurgeryAggressionmedicine.anatomical_structureEndocrinologynervous systemGene Expression RegulationImpulsive BehaviorReceptor Serotonin 5-HT1BConditioning OperantRaphe NucleiCannabinoidRaphe nucleiPsychologyReinforcement Psychology
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Validación de un cuestionario para medir retrospectivamente la exposición laboral a plaguicidas

1997

ResumenObjetivosValidar un cuestionario diseñado para medir retrospectivamente la exposición laboral a plaguicidas en un estudio de casos y controles sobre trabajadores expuestos a plaguicidas y malformaciones congénitas.MétodosSe compara la información obtenida mediante una entrevista personal con 56 agricultores con: 1) una entrevista con los empleadores de los agricultores, 2) la observación directa de los lugares de trabajo y 3) un cuestionario que cumplimentaron los agncultores durante la realización del Programa de Capacitación para Manipuladores de Plaguicidas.ResultadosLos índices de exactitud y fiabilidad fueron altos para las variables «cultivos trabajados», «períodos de tratamien…

Agricultural workersQuestionnairePublic Health Environmental and Occupational HealthExposición laboralPlaguicidasTrabajadores agrícolasValidezOccupational exposurePesticidesCuestionarioValidityGaceta Sanitaria
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Le trasformazioni urbane di Agrigento dai primi decenni del XX secolo agli anni della speculazione edilizia

2018

Come mostra una lunga tradizione di studi di storia urbana, i Catasti storici di un tipo geometrico descrittivo sono oggi proposti come rappresentazioni affidabili di una reale condizione dei luoghi in un determinato momento storico. Nel caso della città di Agrigento, lo studio del Catasto redatto nei primi anni trenta del Novecento, opportunamente supportato dall'aiuto di altre fonti, è la linea di partenza di un itinerario ricostruttivo delle trasformazioni più rilevanti del periodo post-bellico che modificheranno pesantemente la struttura urbana della città, facendo di Agrigento un caso di studio emblematico di quella che era, più in generale, la grave situazione urbanistica dell'intero …

Agrigento historical cadastres historical centre urban transformations urban historyAgrigento catasti storici centro storico trasformazioni urbane storia urbanaSettore ICAR/21 - Urbanistica
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A plant genetically modified that accumulates Pb is especially promising for phytoremediation

2003

6 pages, 3 figures, 1 table.

AgrobacteriumBiophysicsPlant RootsBiochemistryHyperaccumulatorsBioremediationMetals HeavyTobaccoBotanyNicotiana glaucaHyperaccumulatorMolecular BiologyGlucuronidaseNicotianabiologyfungiWild typefood and beveragesBiological TransportCell BiologyPlants Genetically Modifiedbiology.organism_classificationRecombinant ProteinsGenetically modified organismPhytoremediationBiodegradation EnvironmentalLeadSeedlingPhytochelatin synthaseBioremediationCadmium
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Sengrieķu traģēdijas tekstu citējumi Sudas leksikonā: Aishils

2017

Bakalaura darba Sengrieķu traģēdijas tekstu citējumi Sudas leksikonā: Aishils mērķis ir izpētīt Aishila darbu citēšanu Sudas leksikonā un atklāt tās leksikogrāfisko nozīmi. Darbā tiek risināti jautājumi par seno tekstu citēšanas nepieciešamību un tās lomu viduslaiku leksikogrāfiskā darba tapšanā. Darba teorētiskajā daļā tiek skaidrota vārdnīcas, enciklopēdijas un leksikona jēdzienu izpratne, tiek sniegts vispārīgs viduslaiku leksikonu raksturojums, kā arī tiek izklāstīti pētījuma mērķim svarīgie fakti par Sudas leksikonu un Aishila literāro darbību. Praktiskajā daļā ir apskatītas par Aishilu un viņa ģimeni leksikonā atrodamās biogrāfiskās ziņas un tiek analizēti viņa darbu pieminējumi un ci…

AishilsValodniecībasengrieķu traģēdijaviduslaiku leksikogrāfijaSudas leksikonscitējumi
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