Search results for "DAS"
showing 10 items of 4164 documents
Phenoloxidase characterization in vacuolar hemocytes from the solitary ascidian Styela plicata
1995
Phenoloxidase (PO) activity was shown in lysates of Styela plicata hemocytes assayed spectrophotometrically by means of L-Dopa oxidation without divalent cations. Trypsin and chymotrypsin pretreatment and preincubation with microbial lipopolysaccharides significantly activated PO, whereas laminarin or zymosan were ineffective. Soybean trypsin inhibitor, tropolone, and phenylthiourea, but not benzamidine, were inhibitors. Finally, hemocytes were separated by a discontinuous Percoll density gradient to determine which cells were active. PO activity was demonstrated, by both biochemical and cytochemical assays, in the separated fraction enriched mainly with the globular granulocytes called mor…
Estudio sobre la cicatrización de la zona donante de injerto de piel parcial en pacientes quemados tras aplicación de plasma rico en plaquetas o plas…
2019
La técnica más empleada para la restauración de la barrera cutánea en los pacientes quemados es el injerto de piel autólogo de espesor parcial. Su obtención conlleva generar un área cruenta a nivel de otra localización cutánea del mismo paciente, conocida como zona donante. Esta área cura generalmente en un período de entre 7 y 14 días mediante un proceso de cicatrización dirigida. Cuando existen grandes defectos que requieren cobertura, como es el caso de los grandes quemados en los que la extensión de la quemadura limita la disponibilidad de área donante, pueden ser necesarias varias tomas de la misma zona. Por lo tanto, la opción terapéutica global en este tipo de pacientes va a depender…
La paz desde la perspectiva de la sociedad civil (Europa tiene que hacer su paz y propagarla)
2011
Texto basado en la conferencia pronunciada por José Vidal-Beneyto en el "II Congreso Internacional por la Paz en Europa", el 10 de julio de 1996 en el País Vasco. Publicado póstumamente.
La sostenibilidad, un nuevo pensamiento. Intervención de José Vidal-Beneyto
2003
Número monográfico sobre "El nuevo horizonte de la Sostenibilidad". Fundación Santander Central Hispano.
Film noir stila ģenealoģija
2012
Promocijas darba Film noir stila ģenealoģija anotācija Promocijas darba temats Film noir stila ģenealoģija aptver pasaules kinoteorētiskās domas pieredzē aprobēta jēdziena film noir un ar to apzīmētā kinematogrāfiskā mantojuma veidošanos, stilistisko īpatnību un evolūcijas izpēti, tai skaitā – film noir attīstību ASV, Eiropā un arī Latvijā XX–XXI gs. laikmeta sociālpolitisko norišu un modernisma un postmodernisma paradigmas kontekstā. Šis promocijas darbs izseko film noir stila dzimšanai ASV 40.–50. gados ASV, gan arī pētī tās Eiropas un ASV kultūras atskaņas, kuras reflektē filmu kopums, ko dēvē par film noir. Promocijas darbā pētītas film noir naratīvās īpatnības, kas uzskatāmas par alter…
Fluorescence and circular dichroism spectroscopy to understand the interactions between cyclodextrins and α-galactosidase from green coffee beans
2017
Abstract The potential of fluorescence measurement and circular dichroism spectroscopy (CDSP) to evaluate the interaction between cyclodextrins (CDs) (CD cavity size, concentration, pH, reaction time, and temperature as well as different side chain groups) and α-galactosidase was evaluated. A strong relationship was observed between α-galactosidase fluorescence intensity and CD cavity size, concentration, pH, reaction time, and temperature as well as different side chain groups. Therefore, it can be concluded that fluorescence intensity measurement can be a promising tool to ascertain β-CD-α-galactosidase interactions. CDSP is also an interesting tool to understand β-CD-α-galactosidase inte…
Inactivation and structural changes of polyphenol oxidase in quince ( Cydonia oblonga Miller) juice subjected to ultrasonic treatment
2020
Background Polyphenol oxidase (PPO) is considered a problem in the food industry because it starts browning reactions during fruit and vegetable processing. Ultrasonic treatment is a technology used to inactivate the enzyme; however, the mechanism behind PPO inactivation is still unclear. For this reason, the inactivation, aggregation, and structural changes in PPO from quince juice subjected to ultrasonic treatments were investigated. Different intensities and times of ultrasonic treatment were used. Changes in the activity, aggregation, conformation, and structure of PPO were investigated through different structural analyses. Results Compared to untreated juice, the PPO activity in treat…
Existence of metastable intermediate lysozyme conformation highlights the role of alcohols in altering protein stability.
2011
Alcohols have a manifold effect on the conformational and thermodynamic stability of native proteins. Here, we study the effect of moderate concentrations of trifluoroethanol (TFE) on the thermal stability of hen egg-white lysozyme (HEWL), by far-UV circular dichroism and by steady-state and time-resolved photoluminescence of intrinsic tryptophans. Our results highlight that TFE affects lysozyme stability by preferential solvation of the protein molecule. Furthermore, we discovered the existence at 20% TFE of an equilibrium partially folded state of lysozyme, intermediate between the native and the unfolded state. A three-state model is therefore used to interpolate the thermal denaturation…
Piedziņas darbību veikšana pret parādnieka naudas līdzekļiem.
2018
Tiesas sprieduma piespiedu izpilde ir ļoti nozīmīgs un, jāatzīst, arī ļoti komplicēts civilprocesuāls jautājums. Ne vienmēr efektīvais un ātrais nolēmuma izpildes process, kam ir tendence iesaistīt arī ārvalsts elementu, rada nepieciešamību attīstīt un pilnveidot civilprocesuālo regulējumu attiecībā uz sprieduma piespiedu izpildi. Progresējot dažādām mūsdienīgām tehnoloģijām un cilvēku ikdienā ienākot ar vien jauniem līdzekļiem, kas piedāvā atšķirīgās situācijās uzlabotus risinājumus, civilprocess nedrīkst ieņemt stagnējošu stāvokli. Maģistra darba mērķis ir pilnveidot Latvijas civilprocesuālo regulējumu attiecībā uz tiesas nolēmumu piespiedu izpildi, kas vērsta pret juridiskas personas nau…
Substrate determinants for cleavage in cis and in trans by the hepatitis C virus NS3 proteinase
1995
Processing of the hepatitis C virus polyprotein is accomplished by a series of cotranslational and posttranslational cleavages mediated by host cell signalases and two virally encoded proteinases. Of these the NS3 proteinase is essential for processing at the NS3/4A, NS4A/4B, NS4B/5A, and NS5A/5B junctions. Processing between NS3 and NS4A occurs in cis, implying an intramolecular reaction mechanism, whereas cleavage at the other sites can also be mediated in trans. Sequence analysis of the amino termini of mature cleavage products and comparisons of amino acid residues around the scissile bonds of various hepatitis C virus isolates identified amino acid residues which might contribute to su…