Search results for "DAS"
showing 10 items of 4164 documents
Role of two operators in regulating the plasmid-borne raf operon of Escherichia coli
1994
The plasmid-borne raf operon encodes functions required for the inducible uptake and utilization of raffinose in Escherichia coli K12. The expression of three structural genes for alpha-galactosidase (rafA), Raf permease (rafB) and sucrose hydrolase (rafD) is negatively controlled by the binding of RafR repressor (rafR) to two operator sites, O1 and O2, that flank the -35 sequence of the raf promoter, PA. In vitro, O1 and O2 are occupied on increasing the concentration of RafR, without detectable preference for one site or the other or any indication of cooperative binding. Nucleotide substitutions at positions 3, 4 or 5 in an operator half-site prevented repressor binding, supporting a mod…
In vitro antioxidant properties, DNA damage protective activity, and xanthine oxidase inhibitory effect of cajaninstilbene acid, a stilbene compound …
2010
The antioxidant properties, DNA damage protective activities, and xanthine oxidase (XOD) inhibitory effect of cajaninstilbene acid (CSA) derived from pigeon pea leaves were studied in the present work. Compared with resveratrol, CSA showed stronger antioxidant properties, DNA damage protective activity, and XOD inhibition activity. The IC(50) values of CSA for superoxide radical scavenging, hydroxyl radical scavenging, nitric oxide scavenging, reducing power, lipid peroxidation, and XOD inhibition were 19.03, 6.36, 39.65, 20.41, 20.58, and 3.62 μM, respectively. CSA possessed good protective activity from oxidative DNA damage. Furthermore, molecular docking indicated that CSA was more poten…
Vibrio aestivus sp. nov. and Vibrio quintilis sp. nov., related to Marisflavi and Gazogenes clades, respectively
2012
Abstract Two new Vibrio species, Vibrio aestivus and Vibrio quintilis , are described after a polyphasic characterization of strains M22 T , M61 and M62 T , isolated from seawater collected off a beach on the East coast of Spain (Valencia). All three strains are Gram negative, mesophilic, slightly halophilic, fermentative rods. V. aestivus (M22 T = CECT 7558 T = CAIM 1861 T = KCTC 23860 T and M61 = CECT 7559 = CAIM 1862 = KCTC 23861) is oxidase positive, reduces nitrates to nitrites, is negative for Voges Proskauer, arginine dihydrolase and indole and non hydrolytic on most substrates tested. The 16S rRNA gene sequences of M22 T and M61 are most similar to Vibrio marisflavi (97.1–97.2%) …
The disintegrin ADAM9 indirectly contributes to the physiological processing of cellular prion by modulating ADAM10 activity
2005
The cellular prion protein (PrP(c)) is physiologically cleaved in the middle of its 106-126 amino acid neurotoxic region at the 110/111 downward arrow112 peptidyl bond, yielding an N-terminal fragment referred to as N1. We recently demonstrated that two disintegrins, namely ADAM10 and ADAM17 (TACE, tumor necrosis factor alpha converting enzyme) participated in both constitutive and protein kinase C-regulated generation of N1, respectively. These proteolytic events were strikingly reminiscent of those involved in the so-called "alpha-secretase pathway" that leads to the production of secreted sAPPalpha from betaAPP. We show here, by transient and stable transfection analyses, that ADAM9 also…
Axial (apical-basal) expression of pro-apoptotic and pro-survival genes in the lake baikal demosponge Lubomirskia baicalensis.
2006
Like in all other Metazoa, also in sponges (Porifera) proliferation, differentiation, and death of cells are controlled by apoptotic processes, thus allowing the establishment of a Bauplan (body plan). The demosponge Lubomirskia baicalensis from the Lake Baikal is especially suitable to assess the role of the apoptotic molecules, since its grade of construction is highly elaborated into an encrusting base and branches composed of modules lined up along the apical-basal axis. The four cDNAs, ALG-2, BAK, MA-3, and Bcl-2, were isolated from this sponge species. The expression levels of these genes follow characteristic gradients. While the proapoptotic genes are highly expressed at the base of…
Tumor necrosis factor-alpha converting enzyme is processed by proprotein-convertases to its mature form which is degraded upon phorbol ester stimulat…
2003
Tumor necrosis factor-alpha converting enzyme (TACE or ADAM17) is a member of the ADAM (a disintegrin and metalloproteinase) family of type I membrane proteins and mediates the ectodomain shedding of various membrane-anchored signaling and adhesion proteins. TACE is synthesized as an inactive zymogen, which is subsequently proteolytically processed to the catalytically active form. We have identified the proprotein-convertases PC7 and furin to be involved in maturation of TACE. This maturation is negatively influenced by the phorbol ester phorbol-12-myristate-13-acetate (PMA), which decreases the cellular amount of the mature form of TACE in PMA-treated HEK293 and SH-SY5Y cells. Furthermore…
Trimdas latviešu kulturālās aktivitātes Vācijas DP nometnēs pēc Otrā pasaules kara
2019
Šajā bakalaura darbā ir pētīti vairāki vēstures zinātnē izplatīti ar kulturālajām mnemotehnikām saistīti jēdzieni un kulturālās identitātes tradīcijas veidi. Kontekstu, kurā notika pētīšana veido trimdas latviešu kulturālās aktivitātes Vācijas DP nometnēs īsi pēc Otrā pasaules kara. Darbs piedāvā ieskatu trimdas latviešu autoru rakstītu literāro darbu (lugu) izvēlē kā arī īsu pārskatu par Vācijas trimdas latviešu autoru un mākslinieku darbos manāmajām tendencēm tēlotājmākslā un teātrī dotajā vēsturiskajā posmā. Darba uzdevums ir atrast un uzsvērt Vācijas trimdas latviešu māksliniecisku aktivitāšu nozīmi Latvijas un Vācijas vēsturē un kultūrā, jo tā veido abu valstu kultūru svarīgo daļu. Pēt…
Latviešu skautu un gaidu kustība pārvietoto personu nometnēs Rietumvācijā 1945.-1950.
2019
Maģistra darbs “Latviešu skautu un gaidu kustība pārvietoto personu nometnēs Rietumvācijā 1945 - 1950” apskata un raksturo latviešu skautu un gaidu darbību pēc 2. pasaules kara Rietumvācijā, kad daudzi latvieši tika izvietoti pārvietoto personu (DP) nometnēs. Šajā laika periodā skautu un gaidu darbība bija plaši izplatīta, tā bija galvenā jaunatnes organizācijas kustība, kad tajā iesaistījās gandrīz 8000 skautu un gaidu. DP nometņu specifiskie apstākļi, personu ierobežojošie aspekti, latviešu sabiedrības noskaņojums un uzskati un pasaules skautu un gaidu organizāciju uzstādījumi deva gan iespējas, gan arī sagādāja grūtības kustības darbībā, tai nācās spēt pielāgoties jaunajai dzīvei trimdā.…
Manuscritos de humanistas e historiadores (s. XV-XVII) conservados en el fondo mayansiano del patriarca
1987
Nadie puede dudar, en el estado actual de nuestras investigaciones, que uno de los mejores conocedores del humanismo español fue Gregorio Mayans. Sus ediciones de Juan Luis Vives, Francisco Sánchez de las Brozas, Nebrija o Pedro Juan Núñez, lo demuestran con claridad. Más aún, su Specimen bibliothecae hispano-majansianae (Hannover, 1753) evidencia la posesión de obras impresas, pero también de manuscritos, de nuestros mejores humanistas. La biblioteca mayansiana se dispersó y hoy resulta muy difícil encontrar los manuscritos que el erudito utilizara en sus investigaciones. Dado que las grandes bibliotecas (Biblioteca Nacional de Madrid o Biblioteca Municipal de Valencia) disponen de mayores…
Las bibliotecas privadas en los protocolos notariales. Valencia 1780-1808
1984
El trabajo se ha realizado con protocolos notariales de la ciudad de Valencia, entre los años 1740 y 1808, sobre 1 304 inventarios postmortem, incluyendo en ellos algunas divisiones de bienes que llevaban previo el inventario. La mayor parte de los libros de protocolos utilizados se encuentran en el Archivo de Protocolos del Colegio de Corpus Christi de los que se han visto la casi totalidad de escribanos de esas fechas; en menor medida se han utilizado libros del Archivo del Reino de Valencia, en este archivo la selección de notarios ha sido absolutamente aleatoria, salvo en el caso del notario Joaquín Pastor, 1742-1797, del que se tenía noticia de su relación con el grupo mayansiano. En c…