Search results for "DISCOVERY"

showing 10 items of 4119 documents

Quinoline-Based Molecules Targeting c-Met, EGF, and VEGF Receptors and the Proteins Involved in Related Carcinogenic Pathways

2020

The quinoline ring system has long been known as a versatile nucleus in the design and synthesis of biologically active compounds. Currently, more than one hundred quinoline compounds have been approved in therapy as antimicrobial, local anaesthetic, antipsychotic, and anticancer drugs. In drug discovery, indeed, over the last few years, an increase in the publication of papers and patents about quinoline derivatives possessing antiproliferative properties has been observed. This trend can be justified by the versatility and accessibility of the quinoline scaffold, from which new derivatives can be easily designed and synthesized. Within the numerous quinoline small molecules developed as a…

Cell SurvivalAngiogenesisPharmaceutical ScienceAntineoplastic AgentsReviewMolecular Dynamics SimulationAnalytical Chemistrylcsh:QD241-441Structure-Activity Relationship03 medical and health scienceschemistry.chemical_compound0302 clinical medicinelcsh:Organic chemistryEpidermal growth factorquinolineDrug DiscoverySAR studieHumansPhysical and Theoretical Chemistrycarcinogenic pathwaysProtein kinase BPI3K/AKT/mTOR pathway030304 developmental biology0303 health sciencesantiproliferative compoundChemistryDrug discoveryOrganic ChemistryQuinolineBiological activityProto-Oncogene Proteins c-metantiproliferative compoundstargeted therapySettore CHIM/08 - Chimica FarmaceuticaSmall moleculeErbB Receptorscarcinogenic pathwayReceptors Vascular Endothelial Growth FactorSAR studiesChemistry (miscellaneous)030220 oncology & carcinogenesisQuinolinesCancer researchMolecular Medicinekinases modulatorkinases modulatorsbiological dataSignal TransductionMolecules
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Recent advances in the development of cyclin-dependent kinase 7 inhibitors.

2019

Abstract Cyclin dependent kinase 7 (CDK7) plays a double role as it activates several other cyclin dependent kinases and participates to the initiation of transcription. This kinase is overexpressed in various types of tumors. Relatively few selective CDK7 inhibitors have been up to now disclosed. Most of these inhibitors belong to two chemical families: pyrazolopyrimidines and pyrazolotriazines on one side and pyrimidines on another side. They also differ by their molecular mechanism of action. Some are acting as competitive inhibitors and some others are covalent inhibitors. With these tools, the understanding of the potential therapeutic interest of CDK7 inhibitors in cancer is rapidly g…

Cell SurvivalAntineoplastic Agents01 natural sciences03 medical and health sciencesDrug DevelopmentCyclin-dependent kinaseTranscription (biology)Cell Line TumorDrug DiscoverymedicineAnimalsHumansIC50Protein Kinase Inhibitors030304 developmental biologyPharmacology0303 health sciencesbiology010405 organic chemistryChemistryKinaseTriazinesOrganic ChemistryGeneral Medicinemedicine.diseaseCyclin-Dependent Kinases0104 chemical sciencesLeukemiaPyrimidinesbiology.proteinCancer researchMolecular mechanismCyclin-dependent kinase 7Cyclin-Dependent Kinase-Activating KinaseEuropean journal of medicinal chemistry
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Essential oils of Salvia bracteata and Salvia rubifolia from Lebanon: Chemical composition, antimicrobial activity and inhibitory effect on human mel…

2009

Aim of the study: Salvia bracteata Banks et Sol. and Salvia rubifolia Boiss. are known in folk medicine of Lebanon for the treatment of microbial infections, cancer, urinary and pulmonary problems. In the present study the chemical composition and antimicrobial activity of essential oils from aerial parts of Salvia bracteata and Salvia rubifolia collected in Lebanon were evaluated. The oils were also tested for their potential antiproliferative effects against M14 human melanoma cells. Material and methods: The oils were studied by GC and GC-MS and their antibacterial activity (MIC and MBC) was tested against ten bacteria species using the broth dilution method. The inhibitory effect on hum…

Cell SurvivalApoptosisDNA FragmentationMicrobial Sensitivity TestsAntiproliferative activityAntimicrobial activitySalviaPharmacognosyGram-Positive BacteriaEssential oillaw.inventionlawCell Line TumorDrug DiscoveryOils VolatileHumansMTT assaySalviaLebanonMedicinal plantsMelanomaEssential oilCell ProliferationPharmacologybiologyTraditional medicinePlant ExtractsCell MembraneSalvia bracteataAntimicrobial activity Antiproliferative activity Essential oil Salvia bracteata Salvia rubifoliabiology.organism_classificationAntimicrobialAntineoplastic Agents PhytogenicAnti-Bacterial AgentsComet assaySalvia rubifoliaAntibacterial activityPhytotherapyJournal of Ethnopharmacology
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Polyketides from the marine-derived fungus Aspergillus falconensis: In silico and in vitro cytotoxicity studies.

2020

Abstract Fermentation of the marine-derived fungus Aspergillus falconensis, isolated from sediment collected from the Red Sea, Egypt on solid rice medium containing 3.5% NaCl yielded a new dibenzoxepin derivative (1) and a new natural isocoumarin (2) along with six known compounds (3–8). Changes in the metabolic profile of the fungus were induced by replacing NaCl with 3.5% (NH4)2SO4 that resulted in the accumulation of three further known compounds (9–11), which were not detected when the fungus was cultivated in the presence of NaCl. The structures of the new compounds were elucidated by HRESIMS and 1D/2D NMR as well as by comparison with the literature. Molecular docking was conducted fo…

Cell SurvivalClinical BiochemistryDrug Evaluation PreclinicalPharmaceutical ScienceAntineoplastic AgentsBiochemistrychemistry.chemical_compoundMiceStructure-Activity RelationshipCell MovementCell Line TumorDrug DiscoveryAnimalsHumansMTT assayCytotoxicityMolecular BiologyIC50Cell Proliferationchemistry.chemical_classificationbiologyDose-Response Relationship DrugMolecular StructureTopoisomeraseOrganic ChemistryOptical ImagingIn vitroIsocoumarinMolecular Docking SimulationEnzymeAspergillusBiochemistrychemistryPolyketidesbiology.proteinMolecular MedicineFermentationDrug Screening Assays AntitumorBioorganicmedicinal chemistry
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New artesunic acid homodimers: Potent reversal agents of multidrug resistance in leukemia cells

2012

Abstract To evade the problem of multidrug resistance, hybridization of natural products in dimers is considered as an effective method. After the successful synthesis of three artesunic acid homodimers connected by different types of chemical linkers, we analyzed their activity against human CCRF-CEM and multidrug-resistant p -glycoprotein-overexpressing CEM/ADR 5000 leukemia cells and observed, that multidrug resistant cells were not cross-resistant to the new compounds. Collateral sensitivity was observed for artesunic acid homodimer 2. The obtained results deliver valuable information about the linker’s structure which is required for homodimers to be highly cytotoxic.

Cell SurvivalClinical BiochemistryPharmaceutical ScienceAntineoplastic AgentsBiochemistryStructure-Activity RelationshipDrug DiscoveryTumor Cells CulturedmedicineHumansCytotoxic T cellMolecular BiologyArtesunic acidLeukemiaDose-Response Relationship DrugMolecular StructureChemistryOrganic ChemistrySuccinatesmedicine.diseaseArtemisininsDrug Resistance MultipleMultiple drug resistanceLeukemiaBiochemistryDrug Resistance NeoplasmApoptosisMolecular MedicineDrug Screening Assays AntitumorDimerizationLinkerBioorganic & Medicinal Chemistry
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Computational Evaluation and In Vitro Validation of New Epidermal Growth Factor Receptor Inhibitors

2020

Background:The Epidermal Growth Factor Receptor (EGFR) is a transmembrane protein that acts as a receptor of extracellular protein ligands of the epidermal growth factor (EGF/ErbB) family. It has been shown that EGFR is overexpressed by many tumours and correlates with poor prognosis. Therefore, EGFR can be considered as a very interesting therapeutic target for the treatment of a large variety of cancers such as lung, ovarian, endometrial, gastric, bladder and breast cancers, cervical adenocarcinoma, malignant melanoma and glioblastoma.Methods:We have followed a structure-based virtual screening (SBVS) procedure with a library composed of several commercial collections of chemicals (615,46…

Cell SurvivalDrug Evaluation PreclinicalAntineoplastic Agents01 natural sciencesReceptor tyrosine kinaseStructure-Activity Relationship03 medical and health scienceschemistry.chemical_compound0302 clinical medicineErbBEpidermal growth factorCell Line TumorDrug DiscoverymedicineHumansEpidermal growth factor receptorPropidium iodideProtein Kinase InhibitorsCell ProliferationEGFR inhibitorsDose-Response Relationship DrugMolecular StructurebiologyCell growthChemistryGeneral Medicine0104 chemical sciencesErbB ReceptorsMolecular Docking Simulation010404 medicinal & biomolecular chemistry030220 oncology & carcinogenesisbiology.proteinCancer researchErlotinibDrug Screening Assays Antitumormedicine.drugCurrent Topics in Medicinal Chemistry
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Ensemble-based ADME-Tox profiling and virtual screening for the discovery of new inhibitors of the Leishmania mexicana cysteine protease CPB2.8ΔCTE

2018

Abstract: In an effort to identify novel molecular warheads able to inhibit Leishmania mexicana cysteine protease CPB2.8CTE, fused benzo[b]thiophenes and ,'-triketones emerged as covalent inhibitors binding the active site cysteine residue. Enzymatic screening showed a moderate-to-excellent activity (12%-90% inhibition of the target enzyme at 20m). The most promising compounds were selected for further profiling including in vitro cell-based assays and docking studies. Computational data suggest that benzo[b]thiophenes act immediately as non-covalent inhibitors and then as irreversible covalent inhibitors, whereas a reversible covalent mechanism emerged for the 1,3,3'-triketones with a Y-to…

Cell SurvivalLeishmania mexicanaProtozoan ProteinsADME-Tox; Benzo[b]thiophenes; Cysteine protease; Leishmaniasis; TriketonesThiophenesCysteine Proteinase Inhibitors010402 general chemistry01 natural sciencesBiochemistryLeishmania mexicanaCysteine Proteinase InhibitorsCell LineInhibitory Concentration 50Structure-Activity RelationshipCysteine ProteasesCatalytic DomainDrug DiscoveryHumansStructure–activity relationshipcysteine proteaseBinding siteADME-Tox; benzo[b]thiophenes; cysteine protease; leishmaniasis; triketones; Biochemistry; Molecular MedicineBiologyleishmaniasisPharmacologychemistry.chemical_classificationVirtual screeningBinding Sitesbiology010405 organic chemistryPharmacology. TherapyOrganic Chemistrytriketonesbiology.organism_classificationCysteine protease0104 chemical sciencesMolecular Docking SimulationChemistryEnzymeBiochemistrychemistryDocking (molecular)ADME-ToxMolecular Medicinebenzo[b]thiophenes
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Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes

2021

Nucleic acids are essential biomolecules in living systems and represent one of the main targets of chemists, biophysics, biologists, and nanotechnologists. New small molecules are continuously developed to target the duplex (ds) structure of DNA and, most recently, RNA to be used as therapeutics and/or biological tools. Stimuli-triggered systems can promote and hamper the interaction to biomolecules through external stimuli such as light and metal coordination. In this work, we report on the interaction with ds-DNA and ds-RNA of two aza-macrocycles able to coordinate Zn2+ metal ions and form binuclear complexes. The interaction of the aza-macrocycles and the Zn2+ metal complexes with duple…

Cell SurvivalMetal ions in aqueous solutionÀcids nucleicsPharmaceutical Science010402 general chemistryLigands01 natural sciencesArticleAnalytical ChemistryMetalchemistry.chemical_compoundQD241-441Coordination ComplexesCell Line TumorDrug DiscoveryChlorocebus aethiopsAnimalsHumansPhysical and Theoretical ChemistryVero CellsRNA Double-Strandedchemistry.chemical_classification010405 organic chemistryCytotoxinsBiomoleculeOrganic Chemistryzinc complexRNADNASmall moleculeFluorescenceCombinatorial chemistry0104 chemical sciencesZincchemistryChemistry (miscellaneous)visual_artDNA and RNA duplexesvisual_art.visual_art_mediumNucleic acidMolecular MedicineRNAaza-macrocycleDNAMolecules
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Triazolopyridyl ketones as a novel class of antileishmanial agents. DNA binding and BSA interaction

2014

A new series of triazolopyridyl pyridyl ketones has been synthetized by regioselective lithiation of the corresponding [1,2,3]triazolo[1,5-a]pyridine at 7 position followed by reaction with different electrophiles. The in vitro antileishmanial activity of these compounds was evaluated against Leishmaniainfantum, Leishmaniabraziliensis, Leishmaniaguyanensis and Leishmaniaamazonensis. Compounds 6 and 7 were found to be the most active leishmanicidal agents. Both of them showed activities at micromolar concentration against cultured promastigotes of Leishmania spp. (IC₅₀=99.8-26.8 μM), without cytotoxicity on J774 macrophage cells. These two compounds were also tested in vivo in a murine model…

Cell SurvivalPyridinesStereochemistryClinical BiochemistryAntiprotozoal AgentsPharmaceutical SciencePlasma protein bindingBinding CompetitiveBiochemistryCell LineMicechemistry.chemical_compoundIn vivoDrug DiscoveryAnimalsA-DNABovine serum albuminLeishmaniasisMolecular BiologyLeishmaniaQuenching (fluorescence)biologyOrganic ChemistrySerum Albumin BovineDNAKetonesTriazolesIn vitroDisease Models AnimalSpectrometry FluorescenceLiverchemistrybiology.proteinMolecular MedicineCattleTriazolopyridineSpleenDNAProtein BindingBioorganic & Medicinal Chemistry
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Saponins-mediated potentiation of cisplatin accumulation and cytotoxicity in human colon cancer cells.

2002

The triterpene saponins jenisseensosides A, B, C, D were found to increase the accumulation and cytotoxicity of the anticancer agent cisplatin in human colon tumor cells. These compounds are glycosides of quillaic acid whose fucose residue was acylated by a trans- or cis-p methoxycinnamic acid. In contrarst, other saponins derivatives without this acyl moiety were not found to potentiate the accumulation and cytotoxicity of cisplatin. These results suggested the importance of the acyl moiety for activity.

Cell SurvivalSaponinPharmaceutical SciencePlant RootsFucoseAnalytical Chemistrychemistry.chemical_compoundStructure-Activity RelationshipTriterpeneDrug DiscoverymedicineMoietyHumansOleanolic AcidCytotoxicitySilenePlatinumPharmacologychemistry.chemical_classificationCisplatinDose-Response Relationship DrugPlant ExtractsOrganic ChemistryGlycosideBiological activityDrug SynergismSaponinsTriterpenesComplementary and alternative medicinechemistryBiochemistryMolecular Medicinelipids (amino acids peptides and proteins)CisplatinHT29 Cellsmedicine.drugPlanta medica
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