Search results for "DNA Virus Infections"

showing 3 items of 13 documents

Comparative evaluation of molecular methods for the quantitative measure of torquetenovirus (TTV) viremia, the new surrogate marker of immune compete…

2019

Background Torquetenovirus (TTV) viremia is emerging as a promising tool to assess functional immune competence, to predict posttransplant immune-related complications, and eventually to customize immunosuppression. Methods In this study, 327 blood samples were tested using two real-time PCR (rtPCR) assays both targeted to the untranslated region of the TTV genome. The first assay was an in-house rtPCR developed by our group, the second one was the recently marketed TTV R-GENE assay. Results In the validation study, the TTV R-GENE showed good performances in precision and reproducibility, and sensitivity as low as 12 TTV DNA copies/mL, like previously reported for the in-house rtPCR. The Bl…

Concordancedigital droplet PCR; methods comparison; real-time PCR; torquetenovirusViremiaTTVBiologyReal-Time Polymerase Chain ReactionComparative evaluation03 medical and health sciencesTTV; digital droplet PCR; methods comparison; real-time PCR0302 clinical medicineImmune systemVirologymedicineHumans030212 general & internal medicineViremiaDigital droplet pcrTorque teno virusSurrogate endpointReproducibility of Resultsmedicine.diseaseVirologyDNA Virus InfectionsQuantitative measuretorquetenovirusInfectious DiseasesReal-time polymerase chain reactionCase-Control StudiesDNA Viral030211 gastroenterology & hepatologyreal-time PCRImmunocompetencedigital droplet PCRBiomarkersmethods comparison
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Impact of infectious burden on extent and long-term prognosis of atherosclerosis.

2002

Background — Recent findings suggest a causative role of infections in the pathogenesis of atherosclerosis. In hypothesizing an association between infectious agents and the development of atherosclerosis, we would expect a correlation to the extent of atherosclerosis. Moreover, this effect could be multiplied by the number of pathogens to which an individual had been exposed. Methods and Results — In 572 patients, IgG or IgA antibodies to herpes simplex virus 1 and 2, cytomegalovirus, Epstein-Barr virus, Hemophilus influenzae , Chlamydia pneumoniae , Mycoplasma pneumoniae , and Helicobacter pylori were measured. The extent of atherosclerosis was determined by coronary angiography, carotid…

Human cytomegalovirusMalemedicine.medical_specialtyHerpesvirus 4 HumanTime FactorsArteriosclerosisCarotid arteriesCytomegalovirusCoronary diseasemedicine.disease_causeAntibodies ViralHerpesviridaePathogenesisRisk FactorsPhysiology (medical)medicineHumansSimplexvirusIntensive care medicineAgedHelicobacter pyloribusiness.industryBacterial InfectionsChlamydophila pneumoniaeMiddle Agedmedicine.diseasePrognosisAntibodies BacterialHaemophilus influenzaeSurvival AnalysisDNA Virus InfectionsImmunoglobulin AMycoplasma pneumoniaeSurvival RateC-Reactive ProteinLogistic ModelsHerpesvirus hominisImmunoglobulin GImmunologyMultivariate AnalysisVirusesFemaleCardiology and Cardiovascular MedicinebusinessCirculation
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Presence of viruses in wild eels Anguilla anguilla L, from the Albufera Lake (Spain)

2014

A virological analysis was conducted on wild eels from the Albufera Lake (Spain). A total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). Presence of anguillid herpesvirus (AngHV-1), aquabirnavirus and betanodavirus was confirmed by PCR procedures in both surveys, although the number of detections was clearly higher in 2008 (83% of the eels analysed resulted positive for virus presence). AngHV-1 was the viral agent most frequently detected, followed by aquabirnaviruses. Betanodaviruses were detected by the first time in wild eels, and although the detections were only made by nested PCR, high percentage of positives were achieved. In ad…

Malefood.ingredientVeterinary (miscellaneous)Molecular Sequence DataReassortmentBetanodavirusAquatic ScienceBiologyPolymerase Chain ReactionVirusFish DiseasesViral ProteinsRNA Virus InfectionsfoodGenotypeAnimalsRNA VirusesAquabirnavirusPhylogenyPhylogenetic treeDNA VirusesSequence Analysis DNAAnguillabiology.organism_classificationVirologyDNA Virus InfectionsAnguillid herpesvirusSpainFemaleSeasonsNested polymerase chain reactionJournal of Fish Diseases
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