Search results for "DNA polymerase I"

showing 4 items of 14 documents

RNA dependent DNA polymerase in cells of xeroderma pigmentosum

1971

Abstract Cells from X.P. ∗ skin contain an RNA dependent DNA polymerase, while in cells from normal skin this enzyme is lacking. This finding stimulates the thought that carcinogenesis in X.P. cells is due to an infection with an oncogenic RNA virus.

Xeroderma pigmentosumHepatitis B virus DNA polymeraseDNA polymeraseDNA polymerase IIDeoxyribonucleotidesPolynucleotidesBiophysicsRNA-dependent RNA polymeraseTritiummedicine.disease_causeRauscher VirusBiochemistryMicemedicineAnimalsChemical PrecipitationHumansMolecular BiologySkinchemistry.chemical_classificationXeroderma Pigmentosumintegumentary systembiologyRNA virusDNATemplates GeneticCell BiologyRibonucleotidesmedicine.diseasebiology.organism_classificationVirologyMolecular biologyStimulation ChemicalEnzymechemistryAmmonium SulfateDNA Nucleotidyltransferasesbiology.proteinRNAFemaleGuanosine TriphosphateCarcinogenesisBiochemical and Biophysical Research Communications
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Bleomycin, a selective inhibitor of DNA-dependent DNA polymerase from oncogenic RNA viruses.

1972

Abstract Bleomycin, an antibiotic, inhibits the DNA-dependent DNA polymerase from Rauscher murine leukemia virus. Higher concentrations of BLM ∗ are required to inhibit it's RNA-dependent DNA polymerase. These inhibition effects of the non-competitive type are not altered by preincubation of the DNA with BLM. Under comparable conditions neither the DNA-dependent DNA polymerase activity from E. coli and mouse liver nor the DNA-dependent RNA polymerase activity from mouse lymphoma cells are affected by BLM.

congenital hereditary and neonatal diseases and abnormalitiesTime FactorsLymphomaDNA polymeraseHepatitis B virus DNA polymeraseUracil NucleotidesDNA polymerase IIBiophysicsRNA-dependent RNA polymeraseCytosine NucleotidesTritiumBiochemistryRauscher VirusCell LineBleomycinMiceEscherichia coliAnimalsMolecular BiologyPolymeraseDNA clampAntibiotics Antineoplasticbiologyurogenital systemnutritional and metabolic diseasesCell BiologyDNAMolecular biologyReverse transcriptaseKineticsReal-time polymerase chain reactionLiverDNA Nucleotidyltransferasesbiology.proteinRNABiochemical and biophysical research communications
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Activity and kinetics of DNA dependent DNA and RNA polymerases n xeroderma pigmentosum and in normal human skin.

1971

1. DNA dependent DNA polymerase (E.C.2.7.7.7) was prepared from human normal and from Xeroderma pigmentosum skin. 2. DNA polymerase from normal skin has the same Michaelis constant with native and denatured DNA as templateKm= 120 ± 11 µg DNA/ml, with differing maximum reaction velocities. 3. The enzyme from Xeroderma pigmentosum has the same Michaelis constant for denatured DNA as the enzyme from normal skin, but with native DNA as template, theKmvalue is lower (97.2 ± 9.8). The maximum reaction velocities of the Xeroderma pigmentosum enzyme with native resp. denatured DNA as template are the same. 4. DNA dependent RNA polymerases (E.C.2.7.7.6) from normal and Xeroderma pigmentosum skin wer…

congenital hereditary and neonatal diseases and abnormalitiesXeroderma pigmentosumDNA polymeraseDNA polymerase IIDermatologyTritiumEndonucleasechemistry.chemical_compoundmedicineHumansskin and connective tissue diseasesPolymeraseSkinCarbon IsotopesXeroderma PigmentosumDNA clampintegumentary systembiologynutritional and metabolic diseasesRNA NucleotidyltransferasesGeneral MedicineDNAClinical Enzyme Testsmedicine.diseaseMolecular biologyEnzyme ActivationchemistryDNA Nucleotidyltransferasesbiology.proteinPrimer (molecular biology)DNAArchiv fur dermatologische Forschung
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Alterations of DNA-dependent DNA polymerase activities in the immature quail oviduct in response to estrogen stimulation.

1975

Administration of diethylstilbestrol, an estrogen analogue, to immature female quails causes an increase of extractable DNA-dependent DNA polymerase activities from the oviduct. At least two forms of polymerases have been determined, a high molecular weight polymerase (210,000 daltons) and a low molecular weight polymerase (34,000 daltons) calculated from column chromatography Sephadex G-200. During the primary hormone stimulation the amount of extractable enzyme reaches a maximum on the fifth day after daily injections of the hormone. In the period of withdrawal the activities decrease and reach values similar to those determined in the unstimulated oviducts. During secondary stimulation t…

medicine.medical_specialtyDNA polymerasemedicine.drug_classDiethylstilbestrolCoturnixDNA-Directed DNA PolymeraseOviductschemistry.chemical_compoundInternal medicineGeneticsmedicineAnimalsDiethylstilbestrolPolymeraseDNA synthesisbiologyProteinsDNA Polymerase IIDNA Polymerase IStimulation ChemicalEndocrinologychemistryEstrogenbiology.proteinOviductFemaleChromatography columnDNAmedicine.drug
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