Search results for "DNA"
showing 10 items of 6803 documents
TP53 mutations and S-phase fraction but not DNA-ploidy are independent prognostic indicators in laryngeal squamous cell carcinoma
2005
To prospectively evaluate the prognostic significance of TP53, H-, K-, and N-Ras mutations, DNA-ploidy and S-phase fraction (SPF) in patients affected by locally advanced laryngeal squamous cell carcinoma (LSCC). Eight-one patients (median follow-up was 71 months) who underwent resective surgery for primary operable locally advanced LSCC were analyzed. Tumor DNA was screened for mutational analysis by PCR/SSCP and sequencing. DNA-ploidy and SPF were performed by flow cytometric analyses. Thirty-six patients (44%) had, at least, a mutation in the TP53 gene. Of them, 22% (8/36) had double mutations and 3% (1/36) had triple mutations. In total, 46 TP53 mutations were observed. The majority (41…
Regulación del factor transcripcional Whi7 y la proteinquinasa C en el proceso de Start y el checkpoint de integridad del DNA
2019
Start es el principal punto de control del ciclo celular en el que las células se comprometen a una nueva ronda de división celular. Este punto de control implica la activación irreversible de un programa transcripcional por los complejos Cdc28-Cln a través de la inactivación del represor transcripcional de Start Whi5. En este trabajo se proporcionan nuevas claves sobre cómo Whi7, una proteína relacionada a nivel de secuencia con Whi5, reprime Start. Nuestros resultados demuestran que Whi7 es un parálogo genuino de Whi5. Ambas proteínas colaboran en la represión de Start, aunque presentan diferencias importantes en su regulación que convierten a Whi7 en el represor de Start por excelencia e…
Wewnątrznaczyniowe dwuetapowe leczenie tętniaków aorty brzusznej z użyciem Heli-FX EndoAnchor System
2019
Wstęp: Wyniki leczenia tętniaków aorty brzusznej mimo wprowadzenia nowych technik wewnątrznaczyniowych nadal nie zawsze są zadowalające. Szczególnie trudną grupą pacjentów są osoby z krótką oraz stożkową szyją. W tej grupie chorych często występuje w okresie pooperacyjnym przeciek typu I. Jedną z metod mających temu powikłaniu zapobiec jest zastosowanie wkrętów wewnątrznaczyniowych Heli-FX. Ma to zwiększyć przyleganie systemu stentgraftu do ściany aorty brzusznej oraz zapobiec jego ewentualnej migracji. Materiał i metody: Autorzy niniejszego artykułu proponują zastosowanie leczenia dwuetapowego. Pierwszy etap stanowiła implantacja przedłużki aortalnej tuż poniżej odejścia tętnic nerkowych i…
ATTIVITA’ ENZIMATICA NEI SUOLI DELLE SALINE DI TRAPANI E PACECO
2018
Su oltre 80 campioni di suolo costituenti i diversi orizzonti dei suoli prelevati nelle saline di Trapani e Paceco sono state determinate 18 attività enzimatiche idrolasiche e il contenuto di dsDNA.
Molecular analysis of lichen-associated bacterial communities.
2006
The bacterial communities associated with 11 different lichen samples (belonging to eight different species) from different habitats were investigated. The culturable aerobic-heterotrophic fraction of the bacterial communities was isolated from nine lichen samples on protein-rich and sugar-rich/N-free media. Thirty-four bacterial isolates were purified and pooled into groups (phylotypes) by analysis of the ribosomal internal transcribed spacer polymorphism. Twenty five phylotypes were identified, each comprising between one and three isolates. One isolate of each phylotype was partially sequenced and the resulting 16S rRNA gene sequences were compared in a phylogenetic analysis. Three gener…
Complete genome sequence of the methanogenic neotype strain Methanobacterium formicicum MF(T.).
2014
The neotype strain Methanobacterium formicicum MFT (DSM1535), a hydrogenotrophic methanogenic Archaeon, was isolated from a domestic sewage sludge digestor in Urbana (IL, USA). Here, the complete genome sequence of the methanogen is reported. The genome is 2,478,074 bp in size, featuring a GC content of 41.23%. M. formicicum MFT encodes several genes predicted to be involved in adaptation to abiotic stress such as high osmolarity. The strain MFT is of biotechnological importance since M. formicicum strains are often found in production-scale biogas plants and it is suggested as a starter culture for the anaerobic biomethanation process. (C) 2014 Elsevier B.V. All rights reserved.
The value of synthetic linear epitope analogues of La/SSB for the detection of autoantibodies to La/SSB; specificity, sensitivity and comparison of m…
1998
SUMMARY In a previous study it was shown that La/SSB contains four linear epitopes, p147–154, p291–302, p301–318 and p349–364. The aim of the present study was to investigate the value of the synthetic epitope analogues of the La/SSB autoantigen for the detection of antibodies to La/SSB, in comparison with recombinant La and fragments of this protein. A total of 122 sera with anti-La/SSB activity, from patients with primary Sjögren's syndrome (pSS) or systemic lupus erythematosus (SLE), were tested in various peptide-based assays. In addition, 62 sera from pSS or SLE patients with other autoantibody specificities and 95 sera from healthy individuals were used as controls. The autoantibody s…
Taksā d-h.usāyā. Sakramentalny obrzęd pokuty i pojednania w Asyryjskim Kościele Wschodu
2021
Celem niniejszego artykułu jest zaznajomienie czytelnika z teologią sakramentu pokuty i pojednania w Asyryjskim Kościele Wschodu oraz z wykształconą w łonie tej wspólnoty liturgią jego celebracji zwaną „obrzędem pojednania” (taksā d-h.usāyā). Zaprezentowane badania przeprowadzone zostały w oparciu o syryjską tradycję patrystyczną oraz o najważniejsze źródło liturgiczne, jakim w tym wypadku jest obrzęd, którego redakcję przypisuje się katolikosowi Ishā'Yahbowi III (†659). Analiza obejmuje kolejno teologiczny obraz „odzienia chwały”, który tworzy łączność między sakramentem pokuty a chrztem, sens chrześcijańskiego nawrócenia oraz prezentację obrzędu wraz ze zwróceniem uwagi na jego osobliwośc…
ICTV Virus Taxonomy Profile : Finnlakeviridae
2020
Finnlakeviridae is a family of icosahedral, internal membrane-containing bacterial viruses with circular, single-stranded DNA genomes. The family includes the genus, Finnlakevirus, with the species, Flavobacterium virus FLiP. Flavobacterium phage FLiP was isolated with its Gram-negative host bacterium from a boreal freshwater habitat in Central Finland in 2010. It is the first described single-stranded DNA virus with an internal membrane and shares minimal sequence similarity with other known viruses. The virion organization (pseudo T=21 dextro) and major capsid protein fold (double-β-barrel) resemble those of Pseudoalteromonas phage PM2 (family Corticoviridae), which has a double-stranded …
Prometeo genetista. Profitti delle tecniche e metafore della scienza
2016
Il saggio di Claude Calame affronta una tematica di grande rilievo scientifico, ossia il confronto tra il valore epistemologico e sociale dell'uso delle arti tecniche nell'antica Grecia e il valore epistemologico del linguaggio della scienza moderna in particolare applicato all'ambito della genetica e delle moderne biotecnologie e scienze di laboratorio. Lo spunto del confronto è offerto dall'analisi storico-culturale e antropologica del mito di Prometeo, come è stato diversamente declinato dai vari autori dell'antichità classica, a partire da Esiodo sino a Platone, passando per l'autore del dramma "Prometeo incatenato". In una prospettiva di indagine antropopoietica, le arti tecniche perme…