Search results for "DOMAIN"
showing 10 items of 2485 documents
Arsenic trioxide alters the differentiation of mouse embryonic stem cell into cardiomyocytes
2015
AbstractChronic arsenic exposure is associated with increased morbidity and mortality for cardiovascular diseases. Arsenic increases myocardial infarction mortality in young adulthood, suggesting that exposure during foetal life correlates with cardiac alterations emerging later. Here, we investigated the mechanisms of arsenic trioxide (ATO) cardiomyocytes disruption during their differentiation from mouse embryonic stem cells. Throughout 15 days of differentiation in the presence of ATO (0.1, 0.5, 1.0 μM) we analysed: the expression of i) marker genes of mesoderm (day 4), myofibrillogenic commitment (day 7) and post-natal-like cardiomyocytes (day 15); ii) sarcomeric proteins and their orga…
Modulational instability and domain wall solitons in optical fibers
2000
The first part of this thesis presents some theoretical and experimental results about modulational instability and domain wall solitons in bimodal fibers.In the second part is devoted to the interaction of counter-propagating waves in an isotropic optical fiber
A symmetric Galerkin boundary/domain element method for finite elastic deformations
2000
Abstract The Symmetric Galerkin Boundary Element Method (SGBEM) is reformulated for problems of finite elasticity with hyperelastic material and incompressibility, using fundamental solutions related to a (fictitious) homogeneous isotropic and compressible linear elastic material. The proposed formulation contains, besides the standard boundary integrals, domain integrals which account for the problem's nonlinearities through some (fictitious) initial strain and stress fields required to satisfy appropriate “consistency” equations. The boundary/domain integral equation problem so obtained is shown to admit a stationarity principle (a consequence of the Hu-Washizu one), which covers a number…
Dynamic force sensing of filamin revealed in single-molecule experiments
2012
Mechanical forces are important signals for cell response and development, but detailed molecular mechanisms of force sensing are largely unexplored. The cytoskeletal protein filamin is a key connecting element between the cytoskeleton and transmembrane complexes such as integrins or the von Willebrand receptor glycoprotein Ib. Here, we show using single-molecule mechanical measurements that the recently reported Ig domain pair 20–21 of human filamin A acts as an autoinhibited force-activatable mechanosensor. We developed a mechanical single-molecule competition assay that allows online observation of binding events of target peptides in solution to the strained domain pair. We find that fi…
Sorted deduplication: How to process thousands of backup streams
2016
The requirements of deduplication systems have changed in the last years. Early deduplication systems had to process dozens to hundreds of backup streams at the same time while today they are able to process hundreds to thousands of them. Traditional approaches rely on stream-locality, which supports parallelism, but which easily leads to many non-contiguous disk accesses, as each stream competes with all other streams for the available resources. This paper presents a new exact deduplication approach designed for processing thousands of backup streams at the same time on the same fingerprint index. The underlying approach destroys the traditionally exploited temporal chunk locality and cre…
Dynamic Finite Element analysis of fractionally damped structural systems in the time domain
2015
Visco-elastic material models with fractional characteristics have been used for several decades. This paper provides a simple methodology for Finite-Element-based dynamic analysis of structural systems with viscosity characterized by fractional derivatives of the strains. In particular, a re-formulation of the well-known Newmark method taking into account fractional derivatives discretized via the Grunwald–Letnikov summation allows the analysis of structural systems using standard Finite Element technology.
Poincaré inequalities and Steiner symmetrization
1996
A complete geometric characterization for a general Steiner symmetric domain Ω ⊂ Rn to satisfy the Poincare inequality with exponent p > n−1 is obtained and it is shown that this range of exponents is best possible. In the case where the Steiner symmetric domain is determined by revolving the graph of a Lipschitz continuous function, it is shown that the preceding characterization works for all p > 1 and furthermore for such domains a geometric characterization for a more general Sobolev–Poincare inequality to hold is given. Although the operation of Steiner symmetrization need not always preserve a Poincare inequality, a general class of domains is given for which Poincare inequalities are…
Nanog Regulates Primordial Germ Cell Migration Through Cxcr4b
2010
Abstract Gonadal development in vertebrates depends on the early determination of primordial germ cells (PGCs) and their correct migration to the sites where the gonads develop. Several genes have been implicated in PGC specification and migration in vertebrates. Additionally, some of the genes associated with pluripotency, such as Oct4 and Nanog, are expressed in PGCs and gonads, suggesting a role for these genes in maintaining pluripotency of the germ lineage, which may be considered the only cell type that perpetually maintains stemness properties. Here, we report that medaka Nanog (Ol-Nanog) is expressed in the developing PGCs. Depletion of Ol-Nanog protein causes aberrant migration of …
Nanog Regulates Proliferation During Early Fish Development
2009
Abstract Nanog is involved in controlling pluripotency and differentiation of stem cells in vitro. However, its function in vivo has been studied only in mouse embryos and various reports suggest that Nanog may not be required for the regulation of differentiation. To better understand endogenous Nanog function, more animal models should be introduced to complement the murine model. Here, we have identified the homolog of the mammalian Nanog gene in teleost fish and describe the endogenous expression of Ol-Nanog mRNA and protein during medaka (Oryzias latipes) embryonic development and in the adult gonads. Using medaka fish as a vertebrate model to study Nanog function, we demonstrate that …
Mn(II) complexes of scorpiand-like ligands. A model for the MnSOD active centre with high in vitro and in vivo activity
2015
Manganese complexes of polyamines consisting of an aza-pyridinophane macrocyclic core functionalised with side chains containing quinoline or pyridine units have been characterised by a variety of solution techniques and single crystal x-ray diffraction. Some of these compounds have proved to display interesting antioxidant capabilities in vitro and in vivo in prokaryotic (bacteria) and eukaryotic (yeast and fish embryo) organisms. In particular, the Mn complex of the ligand containing a 4-quinoline group in its side arm which, as it happens in the MnSOD enzymes, has a water molecule coordinated to the metal ion that shows the lowest toxicity and highest functional efficiency both in vitro …