Search results for "DOT"

Pašvaldības autonomās un valsts uzdotās kompetences nošķiršanas problemātika

2016

Bakalaura darbā tiek pētītas Latvijas pašvaldību autonomo un valsts uzdoto funkciju nošķiršanas metodes, to nozīme un šo funkciju nenošķiršanas sekas. Kā būtiskākās problēmas darbā apskatītas: 1) nepieciešamība iekļaut pašvaldību funkciju nošķiršanas kritērijus normatīvajā regulējumā, jo tam ir ne tikai teorētiska, bet liela praktiska nozīme, kas, to neizprotot, var izpausties dažādās pašvaldībai negatīvās sekās, 2) normatīvā regulējuma ietekme uz pašvaldību autonomiju dažādu funkciju veikšanā, kā arī 3) normatīvā regulējuma pretrunas ar funkciju klasifikāciju praksē, to veicot pēc nošķiršanas kritērijiem. Darba mērķis ir izstrādāt pašvaldību autonomo un valsts uzdoto funkciju nošķiršanas k…

Efficacy of the antioxidant ebselen in experimental uveitis.

1999

Inflammation results in the production of free radicals. In a model of experimental uveitis upon subcutaneous injection of endotoxin to Lewis rats, i.e., endotoxin-induced experimental uveitis (EIU), we have evaluated the status of the antioxidant capacity of ocular tissues. EIU results in a decrease of glutathione (GSH) content and glutathione peroxidase (GPx) activity in whole eye homogenates 24-h after endotoxin administration. Furthermore, an increase in malondialdehyde (MDA) content was observed in these same samples, thus confirming the involvement of oxidative stress in the pathophysiology of the process. In view of the ability of the antioxidant ebselen as GPx enzyme mimic, we teste…

Cinetica di assorbimento dell’acqua tramite NMR Imaging per la valutazione della efficacia di trattamenti idrorepellenti

2007

L'efficacia dei trattamenti normalmente impiegati per proteggere e/o consolidare materiali lapidei può essere valutata mediante la tecnica di imaging a risonanza magnetica nucleare (MRI), che consente di studiare in modo quantitativo, non invasivo, non distruttivo, la cinetica di assorbimento capillare dell'acqua. In campioni omogenei (Pietra di Lecce) si forma un fronte piatto (con una discontinuità netta alla scala di misura) e la tecnica MRI permette di seguire la cinetica in quanto consente di misurare, in funzione del tempo, l'altezza raggiunta da tale fronte in qualunque sezione interna del campione. Vengono confrontati i risultati ottenuti sullo stesso litotipo utilizzando due divers…

Communication strategies used by 'British Petroleum (BP)' to deal with legitimacy before and after the Deepwater Horizon Accident

2016

Oil spills pose serious threats to the legitimacy of the companies involved and the oil industry in general. The objective of this study is to conduct research on the communication strategies used by ‘British Petroleum’ (BP) to maintain its legitimacy before the Deepwater Horizon (DH) oil spill caused in April 2010 and to repair its legitimacy after the DH accident. There is existing literature on the legitimacy theory and the communication strategies as well as DH accident and its causes. A qualitative study based on the sustainability reports of BP from 2006 to 2015 and its press releases in relation to the DH accident from April 2010 to April 2016 is conducted to discover its preferred c…

Integrative Model for Binding of Bacillus thuringiensis Toxins in Susceptible and Resistant Larvae of the Diamondback Moth (Plutella xylostella)

1999

ABSTRACT Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth ( Plutella xylostella ) is the first insect to evolve resistance to B. thuringiensis in open-field populations. The only known mechanism of resistance to B. thuringiensis in the diamondback moth is reduced binding of toxin to midgut binding sites. In the present work we analyzed competitive binding of B. thuringiensis toxins Cry1Aa, Cry1Ab, Cry1Ac, and Cry1F to brush border membrane vesicles from larval midguts in a susceptib…

Production and characterization of Bacillus thuringiensis Cry1Ac-resistant cotton bollworm Helicoverpa zea (Boddie).

2007

ABSTRACT Laboratory-selected Bacillus thuringiensis -resistant colonies are important tools for elucidating B. thuringiensis resistance mechanisms. However, cotton bollworm, Helicoverpa zea , a target pest of transgenic corn and cotton expressing B. thuringiensis Cry1Ac (Bt corn and cotton), has proven difficult to select for stable resistance. Two populations of H. zea (AR and MR), resistant to the B. thuringiensis protein found in all commercial Bt cotton varieties (Cry1Ac), were established by selection with Cry1Ac activated toxin (AR) or MVP II (MR). Cry1Ac toxin reflects the form ingested by H. zea when feeding on Bt cotton, whereas MVP II is a Cry1Ac formulation used for resistance se…

Comparison of Different Methodologies for Binding Assays of Bacillus thuringiensis Toxins to Membrane Vesicles from Insect Midguts

2002

Occurrence of a common binding site in Mamestra brassicae, Phthorimaea operculella, and Spodoptera exigua for the insecticidal crystal proteins CryIA…

1997

Specific binding to midgut membrane proteins is required for the toxicity of insecticidal crystal proteins (ICP) from Bacillus thuringiensis. A direct relationship between toxicity and binding has been proposed. It has been hypothesized that sharing of a single receptor by more than one ICP could lead to the occurrence of multiple resistance in the event of an alteration in the common receptor. Binding of CryIA(a), CryIA(b) and CryIA(c), three structurally related ICPs, has been studied in Phthorimaea operculella, Mamestra brassicae and, Spodoptera exigua using brush border membrane vesicles (BBMV) from the midgut tissue. Using iodinated CryIA(b), the three insects showed similar results: o…

Genetic variability of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) populations from Latin America is associated with variations in susceptib…

2006

ABSTRACT Bacillus thuringiensis strains isolated from Latin American soil samples that showed toxicity against three Spodoptera frugiperda populations from different geographical areas (Mexico, Colombia, and Brazil) were characterized on the basis of their insecticidal activity, crystal morphology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of parasporal crystals, plasmid profiles, and cry gene content. We found that the different S. frugiperda populations display different susceptibilities to the selected B. thuringiensis strains and also to pure preparations of Cry1B, Cry1C, and Cry1D toxins. Binding assays performed with pure toxin demonstrated that the differences in the …

Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor.

1991

The biochemical mechanism for resistance to Bacillus thuringiensis crystal proteins was studied in a field population of diamondback moths (Plutella xylostella) with a reduced susceptibility to the bioinsecticidal spray. The toxicity and binding characteristics of three crystal proteins [CryIA(b), CryIB, and CryIC] were compared between the field population and a laboratory strain. The field population proved resistant (greater than 200-fold compared with the laboratory strain) to CryIA(b), one of the crystal proteins in the insecticidal formulation. Binding studies showed that the two strains differ in a membrane receptor that recognizes CryIA(b). This crystal protein did not bind to the b…