Search results for "DOWNSTREAM"
showing 9 items of 39 documents
Contingent reward leader behaviour: Where does it come from?
2010
As many academics have pointed out, while researchers have learned a great deal about the effects of contingent reward (CR) leader behaviour, relatively little is known about its genesis. CR transactional leadership is traditionally viewed as an independent variable which exerts influence downstream. However, if we are to understand how to influence, improve or modify these downstream effects, we need to shift our focus upstream towards the study of transactional leadership as a dependent variable. In line with this reasoning, we put forward the hypothesis that a contextual variable (organizational learning capability) and a subordinate's characteristic (employability) are antecedents of CR…
Interbasin Water Transfers in Spain: Interregional Conflicts and Governance Responses
2014
Semiarid and drought-prone Spain has managed to meet ever-increasing water demands for more than 50 years through the construction of publicly funded hydraulic infrastructure. Interbasin water transfers are the most expensive and complex supply-side tool used. They are also the most controversial, often associated with such unintended consequences as deteriorating freshwater ecosystems, disappearing recreational opportunities provided by aquatic ecosystems, and the loss of development opportunities for downstream communities. This situation has become increasingly unstable over the past decade due to the scarcity of new supply augmentation alternatives, political changes involving European …
Functional characterization of the enhancer blocking element of the sea urchin early histone gene cluster reveals insulator properties and three esse…
2000
Insulator elements can be functionally identified by their ability to shield promoters from regulators in a position-dependent manner or their ability to protect adjacent transgenes from position effects. We have previously reported the identification of a 265 bp sns DNA fragment at the 3' end of the sea urchin H2A early histone gene that blocked expression of a reporter gene in transgenic embryos when placed between the enhancer and the promoter. Here we show that sns interferes with enhancer-promoter interaction in a directional manner. When sns is placed between the H2A modulator and the inducible tet operator, the modulator is barred from interaction with the basal promoter. However, th…
Electrotechnologies, microwaves, and ultrasounds combined with binary mixtures of ethanol and water to extract steviol glycosides and antioxidant com…
2017
The aim of this work was to investigate the potential use of non-conventional technologies (microwaves, ultrasounds, pulsed electric fields, and high voltage electrical discharges) to enhance the extraction of high-added value compounds (mainly stevioside and rebaudioside A), and antioxidants (total phenolic compounds, flavonoids, chlorophylls, and carotenoids), from Stevia rebaudiana leaves. After treatment at equivalent energy, a supplementary diffusion was performed until 1 h. Results demonstrated that when water (100%) was used as solvent, the maximum recovery of stevioside (50.8 ± 0.1 mg/g) was obtained after ultrasound-assisted extraction, while microwave allowed the highest yields of…
Optimization of a Biotechnological Process for Production and Purification of Two Recombinant Proteins: Col G and Col H
2017
Different strategies can be used for increasing production of heterologous recombinant proteins in Escherichia coli. Protein size is often critical for obtaining the best quantity/quality ratio of recombinant protein expression. This study focuses on two recombinant proteins; Class I and class II Collagenases, namely Col G and Col H. Their size is about 150 kDa each. We have developed a method to obtain high levels of cell growth and intracellular expression of each Collagenases in recombinant E. coli BL21(DE3). Batch and Fed-batch fermentation procedures have been performed. Results show that Fed-batch technique was most effective in obtaining the highest cell density for each recombinant …
Contratti a valle rimedi civilistici e disciplina della concorrenza
2008
L'entrata in vigore del c.d. regolamento di modernizzazione dell'antitrust europeo (Reg. 1/2003) ha determinato il delinearsi di un sistema misto di enforcement del diritto europeo della concorrenza, ove al tradizionale meccanismo pubblicistico se ne affianca anche uno privatistico. Il volume mette in evidenza le criticità che siffatto impianto di enforcement presenta, tanto dal punto di vista della coerenza complessiva del modello europeo di diritto della concorrenza, quanto dal punto di vista della versatilità dei rimedi del diritto privato ad assolvere ad una qualche funzione di contrasto delle violazioni concorrenziali. Particolare attenzione, entro questa cornice, è dedicata al tema de…
Digital solutions transform the forest-based bioeconomy into a digital platform industry : a suggestion for a disruptive business model in the digita…
2018
With the notion that the transformation of the forest-based bioeconomy in recent years provides insightful suggestions not only on the bioeconomy, but on business innovation, this paper conducts an empirical analysis of the transformation and attempts to extract suggestions for a digital-solution-driven, disruptive business model in the digital economy. Notwithstanding the potential broad cross-sectoral benefits, the natural environment, locality constraints, and incessant challenge of distance have impeded the balanced development of the bioeconomy. However, driven by digital solutions, the bioeconomy has taken big steps forward in recent years. Digitalization has enabled real-time, end-to…
River-bed erosion due to changing boundary conditions: performance of a protective measure
2018
Abstract. Due to the introduction of man-made sediment barriers along a river, the amount of sediment load entering the downstream river reach is different to that leaving the reach, and erosion processes occur downstream of the barrier itself. Designers are often required to take into account the scouring process and to include adequate protective measures against the local scour. This paper addresses the performance of bio-engineering protective measures against the erosion process. In particular, a green carpet, realized with real flexible vegetation, has been used as the protective measure against erosion processes downstream of a rigid bed. Analyses are based on experimental work carri…
Isolation of nuclei and downstream processing of cell-type-specific nuclei from micro-dissected mouse brain regions – techniques and caveats
2020
AbstractThe mammalian brain consists of several structurally and functionally distinct regions equipped with an equally complex cell-type system. Due to its relevance in uncovering disease mechanisms, the study of cell-type-specific molecular signatures of different brain regions has increased. The rapid evolution of newer and cheaper sequencing techniques has also boosted the interest in cell-type-specific epigenetic studies. In fact, the nucleus holds most of the cell’s epigenetic information and is quite resistant to tissue dissociation processes as compared to cells. As such, nuclei are continually preferred over cells for epigenetic studies. However, the isolation of nuclei from cells …