Search results for "Decidua"
showing 10 items of 26 documents
Unpredicted ovulations and conceptions during early pregnancy: an explanatory mechanism of human superfetation
2012
In this bioessay, a literature review on human superfetation was performed in order to find epidemiological variables associated with this phenomenon. Thereafter, an explanatory mechanism of superfetation compatible with the endocrinological, histological and physiological changes undergone by women during early pregnancy is proposed. Superfetation can be defined as the ovulation, fertilisation and implantation of a second or additional embryo(s) during pregnancy. The literature review evidences a small discordance in gestational age between dizygotic twins in humans (range: 2–4 weeks; mean ± s.e.m.: 3.3 ± 0.3 weeks). This difference is compatible with a luteal out-of-phase (LOOP; i.e. atyp…
Expresión, localización y estudio funcional de PGRMC1 en el endometrio humano a lo largo del ciclo menstrual
2018
Las acciones de la progesterona (P4), en el sistema reproductivo femenino, están principalmente mediadas por los receptores clásicos de la progesterona (PR), que actúan como factores de transcripción. Sin embargo, no todas las acciones fisiológicas de la progesterona pueden explicarse por la activación de dichos receptores. De hecho, se conoce que la progesterona actúa también a través de vías de señalización no clásica más rápidas, mediadas por la activación de receptores de la membrana celular. Entre los receptores no clásicos de la progesterona encontramos PGRMC1 (Progesterone receptor membrane component 1), cuyo mecanismo de acción queda por ser definido. La interacción P4-PGRMC1 media …
Proprotein convertase 5/6 is critical for embryo implantation in women: regulating receptivity by cleaving ebp50, modulating ezrin binding, and membr…
2011
Establishment of endometrial receptivity is vital for successful embryo implantation; its failure causes infertility. Epithelial receptivity acquisition involves dramatic structural changes in the plasma membrane and cytoskeleton. Proprotein convertase 5/6 (PC6), a serine protease of the proprotein convertase (PC) family, is up-regulated in the human endometrium specifically at the time of epithelial receptivity and stromal cell decidualization. PC6 is the only PC member tightly regulated in this manner. The current study addressed the importance and mechanisms of PC6 action in regulating receptivity in women. PC6 was dysregulated in the endometrial epithelium during the window of implantat…
miRNA Signature and Dicer Requirement during Human Endometrial Stromal Decidualization In Vitro
2012
Decidualization is a morphological and biochemical transformation of endometrial stromal fibroblast into differentiated decidual cells, which is critical for embryo implantation and pregnancy establishment. The complex regulatory networks have been elucidated at both the transcriptome and the proteome levels, however very little is known about the post-transcriptional regulation of this process. miRNAs regulate multiple physiological pathways and their de-regulation is associated with human disorders including gynaecological conditions such as endometriosis and preeclampsia. In this study we profile the miRNAs expression throughout human endometrial stromal (hESCs) decidualization and analy…
Preeclampsia: a defect in decidualization is associated with deficiency of Annexin A2.
2020
Background Decidualization defects in the endometrium have been demonstrated at the time of delivery in women with severe preeclampsia and to linger for years, which suggests a maternal contribution to the pathogenesis of this condition. Global transcriptional profiling reveals alterations in gene expression, which includes down-regulation of Annexin A2 in severe preeclampsia patients with decidualization resistance. Objective We investigated the functional role of Annexin A2 deficiency during endometrial decidualization and its potential contribution to shallow trophoblast invasion during implantation and subsequent placentation using in vitro and in vivo modeling. Study Design Annexin A2 …
Papel de la familia notch en la angiogénesis ovarica y uterina en condiciones fisiológicas y patológicas
2014
La angiogénesis es el proceso por el cual se generan nuevos vasos sanguíneos a partir de vasos pre-existente y es esencial para el desarrollo y supervivencia de los seres vivos. En el embrión se produce una angiogénesis muy importante con el fin de formar el sistema vascular del nuevo individuo en su totalidad. Sin embargo, y aunque en adultos en endotelio conserva su potencial angiogénico durante toda la vida del individuo, en condiciones fisiológicas generalmente permanece quiescente. Solo se activa en casos concretos como en procesos de curación de heridas y en órganos con angiogénesis cíclica, es decir, los órganos reproductores: el ovario y el endometrio. También se activa en condicion…
A novel homologous model for noninvasive monitoring of endometriosis progression.
2017
To date, several groups have generated homologous models of endometriosis through the implantation of endometrial tissue fluorescently labeled by green fluorescent protein (GFP) or tissue from luciferase-expressing transgenic mice into recipient animals, enabling noninvasive monitoring of lesion signal. These models present an advantage over endpoint models, but some limitations persist; use of transgenic mice is laborious and expensive, and GFP presents poor tissue penetration due to the relatively short emission wavelength. For this reason, a homologous mouse model of endometriosis that allows in vivo monitoring of generated lesions over time and mimics human lesions in recipient mice wou…
Patrón de decidualización en mujeres con preeclampsia mediante la aplicación de métodos de transcriptómica y proteómica
2015
Los defectos en la decidualización de las células del estroma endometrial humanas (CEE) están relacionados con la alteración en la invasión trofoblástica y en las alteraciones fibrinolíticas, siendo una posible causa de la preeclampsia. El objetivo de esta tesis doctoral es investigar el proceso de decidualización en pacientes que han sufrido preeclampsia severa y la implicación de la anexina A2 (ANXA2) como regulador clave de la invasión defectuosa del trofoblasto y las alteraciones fibrinolíticas de esta enfermedad. Material y Métodos: CEE procedentes de mujeres que han sufrido preeclampsia grave (PE) (N = 13) y pacientes con antecedentes de embarazos sanos (Non-PE) (N = 13) fueron decidu…
Mecanismos epigenéticos en las células endometriales estromales durante el proceso de decidualización
2012
Durante el ciclo menstrual y bajo el control de las hormonas esteroideas el endometrio experimenta grandes cambios morfológicos y bioquímicos en preparación para la implantación embrionaria. Los principales eventos que tienen lugar son el establecimiento del estado receptivo seguido de un aumento en los niveles de progesterona que induce la decidualización de las células estromales endometriales. La decidualización es una transformación morfológica y funcional del compartimento endometrial estromal necesaria para el control del proceso de invasión embrionaria y la formación de la placenta y por lo tanto para el establecimiento del embarazo. Este proceso está muy bien caracterizado a nivel t…
Inhibition of Delta-like 4 mediated signaling induces abortion in mice due to deregulation of decidual angiogenesis.
2013
Objective: To explore whether the Dll4/Notch1 pathway plays a key role in regulating the vascular endothelial growth factor (VEGF)/VEGF receptor 2 (VEGFR2) driven decidual angiogenesis and related pregnancy through induction of a tip/stalk phenotype. Methods: Progesterone-replaced ovariectomized pregnant mice received a single injection of YW152F (Dll4 blocking antibody, BAb) or placebo at embryonic day (E) 4.5. Animals were sacrificed at different time points; blood and uterus were collected for further analysis. Number of embryos and implantation site, uteri weight, and serum progesterone levels were assessed. Alterations in the tip/stalk phenotype were determined by quantitative immunofl…