Search results for "Development"
showing 10 items of 26949 documents
Symbiosis between river and dry lands: phycobiont dynamics on river gravel bars
2020
AbstractRiver gravel bars are dynamic and heterogeneous habitats standing on transition between aquatic and terrestrial environment. Periodical flooding, low nutrient content, frost, missing safe sites, drought, and heat on the ground surface significantly influence life in these habitats. Mutualistic symbiosis may be a successful strategy for organisms to survive and to proliferate under harsh conditions. The lichen genus Stereocaulon was selected as a model symbiotic system among the organisms living on river gravel bars. The aim of our work was to determine effect of this dynamic environment on a phycobiont (i.e., green eukaryotic photobiont) community structure. We analysed 147 Stereoca…
Localization microscopy of DNA in situ using Vybrant(®) DyeCycle™ Violet fluorescent probe: A new approach to study nuclear nanostructure at single m…
2016
Higher order chromatin structure is not only required to compact and spatially arrange long chromatids within a nucleus, but have also important functional roles, including control of gene expression and DNA processing. However, studies of chromatin nanostructures cannot be performed using conventional widefield and confocal microscopy because of the limited optical resolution. Various methods of superresolution microscopy have been described to overcome this difficulty, like structured illumination and single molecule localization microscopy. We report here that the standard DNA dye Vybrant(®) DyeCycle™ Violet can be used to provide single molecule localization microscopy (SMLM) images of …
Novel, automated, semi-industrial modular photobioreactor system for cultivation of demanding microalgae that produce fine chemicals - The next story…
2021
Abstract Recently, there has been increased interest in the use of microorganisms in the production of pharmaceuticals, nutraceuticals and energy supply products, which is due to their rapid growth rate and ability to biosynthesize fine chemicals or biotransform specific xenobiotics. To achieve the desired scale of production and optimization of microbial cultures, it is necessary to design bioreactors that enable process automation, control of working parameters, reduction of microbial and chemical contaminations, and culture independence of climate conditions. In response to this need, an original, modular airlift-type photobioreactor system was designed and manufactured. This novel semit…
Testing of wireless sensor performance in Vessel-to-Vessel Motion Compensation
2016
Relative Vessel-to-Vessel Motion Compensation (VVMC) between two floating vessels can be considered an extension of existing Active Heave Compensation (AHC), which is current state-of-the-art in the offshore industry. In this paper, the experimental results from a small-scale experimental setup of a VVMC scenario are presented. The work focuses on the kinematics related to VVMC, sensor accuracy of Motion Reference Units (MRUs), and the communication delay related to wireless transmission of MRU data between two floating vessels. The accuracies of the MRU sensors are good and verified to be less than 5% of the heave amplitude. The wireless communication link had a typical time-delay of 8ms. …
A time-varying observer for linear systems with asynchronous discrete-time measurements
2017
International audience; In this paper we propose a time-varying observer for a linear continuous-time plant with asynchronous discrete-time measurements. The proposed observer is contextualized in the hybrid systems framework providing an elegant setting for the proposed solution. In particular some theoretical tools are provided, in terms of LMIs, certifying asymptotic stability of a certain compact set where the estimation error is zero. Moreover the case of asynchronous measurements is considered, i.e. when the measurements are not provided in well defined time instants, but they occur at an arbitrary time in a certain time interval. A design procedure based on the numerical solution of …
Der Zellkern - eine Stadt in der Zelle
2016
Gespeicherte genetische Information allein nutzt nichts, wenn sie nicht zur richtigen Zeit und am richtigen Ort abgerufen werden kann. Die Verpackung der DNA im Chromatin des Zellkerns und dessen dynamische, raum-zeitliche Anordnung haben einen entscheidenden Einfluss auf die Genexpression und weitere Funktionen des Zellkerns. In dieser Ubersichtsarbeit beschreiben die Verfasser die Entwicklung der experimentellen Zellkernarchitektur-Forschung und die damit einhergehenden Veranderungen der Vorstellungen zur funktionellen Organisation des Zellkerns.
La evolución de la anatomía patológica: hacia dónde nos dirigimos
2017
Una visión integral del cáncer (I). Microambiente tumoral: estudio, clasificación y reprogramación
2019
Resumen El grupo de enfermedades al que nos referimos como «cancer» comparte una estructura biologica conformada por un ecosistema complejo, donde se han alterado las relaciones intercelulares, los campos de informacion, el desarrollo y la funcion tisular. Mas alla de las alteraciones geneticas de la celula tumoral, la demostracion de un ecosistema alterado, con sus interconexiones a nivel sistemico, abre una nueva perspectiva de la biologia y del comportamiento del cancer. Diversas facetas del tumor, su morfologia, clasificacion, agresividad clinica, pronostico y respuesta al tratamiento aparecen ahora bajo una vision integral que ofrece un nuevo horizonte de estudio, investigacion y manej…
Sarcoma fibromixoide de bajo grado, un diagnóstico diferencial esencial en los tumores mixoides de apariencia benigna
2018
Resumen El sarcoma fibromixoide de bajo grado es una neoplasia poco frecuente que afecta preferentemente a adultos jovenes. Se trata de un tumor fibroblastico de apariencia enganosamente benigna que puede presentar recurrencias locales y metastasis a distancia tardias. Presentamos el caso de una nina de 11 anos que presentaba una lesion en el muslo, de la que se realizo exeresis completa. El estudio histologico mostro una neoplasia bien delimitada de estroma mixoide, con celularidad fusiforme sin atipia ni mitosis. La expresion inmunohistoquimica de MUC4, junto con el estudio de biologia molecular que detecto la expresion del gen de fusion FUS-CREB3L2, confirmaron el diagnostico de sarcoma …
Rabdomiosarcoma alveolar. Dos casos negativos para la fusión PAX3-FOXO1 y PAX7-FOXO1
2022
Resumen Los rabdomiosarcomas son los tumores de partes blandas mas frecuentes en la edad pediatrica y en adultos jovenes. Morfologicamente se distinguen dos subtipos principales: el rabdomiosarcoma alveolar y el rabdomiosarcoma embrionario. El subtipo alveolar se asocia generalmente con un peor pronostico y presenta una fusion genica caracteristica que clasicamente se ha utilizado para confirmar el diagnostico: PAX3/7-FOXO1. Presentamos dos casos que fueron remitidos a nuestro centro. Ambos mostraron morfologia alveolar clasica y diferenciacion muscular basada en la inmunohistoquimica; no obstante, carecian de la fusion caracteristica PAX3/7-FOXO1. El objetivo de este articulo es resaltar l…