Search results for "Diagnostic medicine"

showing 10 items of 213 documents

Anti-angiogenic drug loaded liposomes: Nanotherapy for early atherosclerotic lesions in mice.

2018

Este artículo se encuentra disponible en la página web de la revista en la siguiente URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0190540 También participan en la elaboración de este artículo científico: Aracely Calatayud-Pascual, Alicia López-Castellano, Elena P. Albelda, Enrique García-España, Luis Martí-Bonmatí, Juan C. Frias y M. Teresa Albelda. Fumagillin-loaded liposomes were injected into ApoE-KO mice. The animals were divided into several groups to test the efficacy of this anti-angiogenic drug for early treatment of atherosclerotic lesions. Statistical analysis of the lesions revealed a decrease in the lesion size after 5 weeks of treatment.

Fluorescence-lifetime imaging microscopyPathologylcsh:MedicineAngiogenesis Inhibitors02 engineering and technology030204 cardiovascular system & hematologyVascular MedicineBiochemistryArteriosclerosis - Chemotherapy.Diagnostic RadiologyAteroesclerosis - Farmacoterapia.MiceWhite Blood Cells0302 clinical medicineAnimal CellsArteriosclerosis - Farmacoterapia.Medicine and Health SciencesArteries - Diseases - Treatment.Nanotechnologylcsh:ScienceAortaPhospholipidsmedia_commonMice KnockoutLiposomeDrug CarriersMultidisciplinarymedicine.diagnostic_testRadiology and Imaging021001 nanoscience & nanotechnologyMagnetic Resonance ImagingLipidsFatty Acids UnsaturatedEngineering and Technologymedicine.symptomCellular Structures and OrganellesCellular TypesAnatomy0210 nano-technologySesquiterpenesResearch ArticleDrugmedicine.medical_specialtyImaging Techniquesmedia_common.quotation_subjectImmune CellsImmunologyLiposomes.Research and Analysis MethodsLiposomas.Lesion03 medical and health sciencesText miningApolipoproteins ECyclohexanesDiagnostic Medicinemedicine.arteryFluorescence ImagingmedicineAnimalsArterias - Enfermedades - Tratamiento.VesiclesAortaBlood Cellsbusiness.industryMacrophageslcsh:RAnti angiogenicBiology and Life SciencesMagnetic resonance imagingCell BiologyAtherosclerosisFumagillin - Therapeutic use.Atherosclerosis - Chemotherapy.Disease Models AnimalFumagilina - Uso terapéutico.LiposomesCardiovascular AnatomyNanoparticlesBlood Vesselslcsh:QbusinessPloS one
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Polish is quantitatively different on quartzite flakes used on different worked materials.

2020

Metrology has been successfully used in the last decade to quantify use-wear on stone tools. Such techniques have been mostly applied to fine-grained rocks (chert), while studies on coarse-grained raw materials have been relatively infrequent. In this study, confocal microscopy was employed to investigate polished surfaces on a coarse-grained lithology, quartzite. Wear originating from contact with five different worked materials were classified in a data-driven approach using machine learning. Two different classifiers, a decision tree and a support-vector machine, were used to assign the different textures to a worked material based on a selected number of parameters (Mean density of furr…

Future studiesConfocal MicroscopyDecision AnalysisLithologyRaw MaterialsAntlersBone imagingPlant Science01 natural sciencesDiagnostic RadiologyMedicine and Health Sciences0601 history and archaeologyElectron MicroscopyAnimal AnatomyMaterialsMicroscopyMultidisciplinary060102 archaeologyPlant AnatomyRadiology and ImagingQRLight Microscopy06 humanities and the artsQuartzWoodBone ImagingProcess EngineeringPhysical SciencesMedicineEngineering and TechnologyScanning Electron MicroscopyAnatomyManagement EngineeringGeologyResearch Article010506 paleontologyImaging TechniquesScienceMaterials ScienceMineralogyIndustrial ProcessesResearch and Analysis MethodsDiagnostic MedicineIndustrial Engineering0105 earth and related environmental sciencesSurface TreatmentsDecision TreesBiology and Life SciencesManufacturing ProcessesSample size determinationZoologyPloS one
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Direct quantification of cell-free, circulating DNA from unpurified plasma.

2014

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is known to be accompanied by a reduction of DNA yield. The primer sets were designed to amplify a 90 and 222 bp multi-locus L1PA2 sequence. In the first module, cfDNA concentrations in unpurified plasma were compared to cfDNA concentrations in the eluate and the flow-through of the QIAamp DNA Blood Mini Kit and in the eluate of a phenol-chloroform isoamyl (PCI) based DNA extraction, to elucidate the D…

Gene Identification and Analysislcsh:MedicineCoronary DiseaseReal-Time Polymerase Chain ReactionBiochemistrylaw.inventionMolecular Geneticschemistry.chemical_compoundDiagnostic MedicinelawNucleic AcidsMolecular Cell BiologyBlood plasmaGeneticsHumanslcsh:ScienceExerciseBiologyPolymerase chain reactionDNA PrimersPlasma ProteinsMultidisciplinaryBase SequenceCell-Free SystemChemistrylcsh:RProteinsDNAMolecular biologyDNA extractionCoronary heart diseaseReal-time polymerase chain reactionCase-Control StudiesRNAMedicineCirculating DNAlcsh:QGene expressionGene FunctionPrimer (molecular biology)DNA modificationDNAResearch ArticlePLoS ONE
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Mediterranean diet adherence and synergy with acute myocardial infarction and its determinants: A multicenter case-control study in Italy

2018

Background Cardiovascular diseases are the leading causes of mortality and morbidity in Western countries. The possible synergistic effect of poor adherence to a Mediterranean diet (MD) and other risk factors for acute myocardial infarction (AMI) such as hypertension, cholesterol, ever smoker, BMI> 25, diabetes, has not been deeply studied. Design Case-control study. Methods Patients with first AMI and controls from four tertiary referral Italian centers were screened for enrolment. Dietary information was collected through a questionnaire and a MD adherence score was calculated. Physical activity and smoking habits were also registered. The Synergy Index was calculated according to Rothman…

Genetics and Molecular Biology (all)MaleMultivariate analysisMediterranean dietTertiary Care CenterMyocardial Infarctionlcsh:MedicineBlood PressureMediterranean030204 cardiovascular system & hematologyPathology and Laboratory MedicineDiet MediterraneanVascular MedicineBiochemistryGeographical locationsTertiary Care CentersHabitschemistry.chemical_compoundEndocrinology0302 clinical medicineRisk FactorsSurveys and QuestionnairesMedicine and Health SciencesSmoking HabitsSurveys and Questionnaire030212 general & internal medicineMyocardial infarctionlcsh:ScienceMultidisciplinarySmokingMiddle AgedEuropeHyperlipidemiaItalyResearch DesignSettore MED/42HypertensionFemaleCase-Control StudieHumanResearch Articlemedicine.medical_specialtyAged; Case-Control Studies; Feeding Behavior; Female; Humans; Hypertension; Italy; Male; Middle Aged; Myocardial Infarction; Risk Factors; Smoking; Surveys and Questionnaires; Tertiary Care Centers; Diet Mediterranean; Patient ComplianceReferralEndocrine DisordersSmoking habitHypercholesterolemiaCardiologyResearch and Analysis Methods03 medical and health sciencesSigns and SymptomsDiagnostic MedicineDiabetes mellitusInternal medicineDiabetes MellitusAged; Case-Control Studies; Feeding Behavior; Female; Humans; Hypertension; Italy; Male; Middle Aged; Myocardial Infarction; Risk Factors; Smoking; Surveys and Questionnaires; Tertiary Care Centers; Diet Mediterranean; Patient Compliance; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)medicineHumansEuropean UnionNutritionAgedBehaviorCholesterolbusiness.industryRisk Factorlcsh:RCase-control studyBiology and Life SciencesFeeding Behaviormedicine.diseaseDietAgricultural and Biological Sciences (all)chemistryMetabolic DisordersCase-Control StudiesPatient Compliancelcsh:QPeople and placesbusinessPLOS ONE
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Factors influencing psychological well-being in patients with Parkinson's disease.

2017

Background Both motor and non-motor symptoms could contribute to significant deterioration of psychological well-being in patients with Parkinson's disease (PD). However, its assessment has been only indirectly evaluated using tools based on health-related quality of life (HRQoL), such as the PDQ-39 scale. Objectives To evaluate psychological well-being in PD using a specific tool of assessment, the Psychological Well-being Scale (PWS), and its clinical correlates. Methods This article reports data of patients' perception of health state, as measured by means of the PWS, from an epidemiological, cross-sectional study conducted in Italian PD patients (FORTE Study). We tested possible relatio…

Genetics and Molecular Biology (all)MaleParkinson's diseaseEmotionsPoison controlSocial Scienceslcsh:MedicinePathology and Laboratory MedicineBiochemistrySeverity of Illness IndexAged; Depression; Fatigue; Female; Humans; Italy; Male; Middle Aged; Parkinson Disease; Psychiatric Status Rating Scales; Quality of Life; Severity of Illness Index; Surveys and Questionnaires; Biochemistry Genetics and Molecular Biology (all); Agricultural and Biological Sciences (all)0302 clinical medicineMathematical and Statistical TechniquesQuality of lifeSurveys and QuestionnairesMedicine and Health SciencesMedicinePsychologySurveys and Questionnaire030212 general & internal medicinelcsh:ScienceDepression (differential diagnoses)FatigueMultidisciplinaryMovement DisordersDepressionNeurodegenerative DiseasesParkinson DiseaseMiddle AgedPsychiatric Status Rating ScalehumanitiesNeurologyItalyPhysical SciencesFemaleStatistics (Mathematics)Clinical psychologyResearch ArticleHumanResearch and Analysis Methods03 medical and health sciencesSigns and SymptomsDiagnostic MedicineSeverity of illnessInjury preventionMental Health and PsychiatryHumansStatistical MethodsAgedPsychiatric Status Rating Scalesparkinson's disease quality of lifeAnalysis of VarianceBiochemistry Genetics and Molecular Biology (all)business.industryMood Disorderslcsh:RBeck Depression InventoryBiology and Life Sciencesmedicine.diseaseHealth CareAgricultural and Biological Sciences (all)Psychological well-beingAged; Depression; Fatigue; Female; Humans; Italy; Male; Middle Aged; Parkinson Disease; Psychiatric Status Rating Scales; Quality of Life; Severity of Illness Index; Surveys and QuestionnairesQuality of Lifelcsh:QbusinessSleep Disorders030217 neurology & neurosurgeryMathematicsPLoS ONE
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Correction: Recovery from Toxic-Induced Demyelination Does Not Require the NG2 Proteoglycan.

2018

[This corrects the article DOI: 10.1371/journal.pone.0163841.].

ImmunologyGene ExpressionMouse ModelsCell MigrationResearch and Analysis MethodsPathology and Laboratory MedicineCorpus CallosumDirected Cell MigrationModel OrganismsNerve FibersSigns and SymptomsAnimal CellsDiagnostic MedicineMedicine and Health SciencesGeneticsImmune ResponseNeuronsInflammationChemotaxisBiology and Life SciencesBrainCell DifferentiationAnimal ModelsCell BiologyAxonsCell MotilityCellular NeuroscienceCellular TypesAnatomyResearch ArticleDevelopmental BiologyNeurosciencePloS one
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NT pro BNP plasma level and atrial volume are linked to the severity of liver cirrhosis.

2013

BACKGROUND AND AIMS: Plasma levels of NT-pro-BNP, a natriuretic peptide precursor, are raised in the presence of fluid retention of cardiac origin and can be used as markers of cardiac dysfunction. Recent studies showed high levels of NT pro BNP in patients with cirrhosis. We assessed NT pro-BNP and other parameters of cardiac dysfunction in patients with cirrhosis, with or without ascites, in order to determine whether the behaviour of NT pro BNP is linked to the stage of liver disease or to secondary cardiac dysfunction. METHODS: Fifty eight consecutive hospitalized patients mostly with viral or NAFLD-related cirrhosis were studied. All underwent abdominal ultrasound and upper GI endoscop…

Liver CirrhosisMaleCirrhosisAnatomy and Physiologylcsh:MedicineCardiovascularCardiovascular SystemLiver diseaseAscitesNatriuretic Peptide BrainNatriuretic peptidePathologylcsh:ScienceMultidisciplinaryLiver DiseasesMiddle AgedHypertensioncardiovascular systemCardiologyMedicineFemalemedicine.symptomhormones hormone substitutes and hormone antagonistscirculatory and respiratory physiologyResearch Articlemedicine.medical_specialtymedicine.drug_classGastroenterology and HepatologyCardiac dysfunctionDiagnostic MedicineInternal medicinemedicineHumanscardiovascular diseasesHeart Atriabusiness.industryAcute Cardiovascular Problemscirrhosislcsh:RCase-control studyPlasma levelsmedicine.diseasePeptide FragmentsEndocrinologyCase-Control Studieslcsh:QN terminal pro b type natriuretic peptidebusinesshuman activitiesBiomarkersGeneral Pathology
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Immune-inflammatory and metabolic effects of high dose furosemide plus hypertonic saline solution (HSS) treatment in cirrhotic subjects with refracto…

2016

Introduction Patients with chronic liver diseases are usually thin as a result of hypermetabolism and malnutrition expressed by reduced levels of leptin and impairment of other adyponectins such as visfatin. Aims We evaluated the metabolic and inflammatory effects of intravenous high-dose furosemide plus hypertonic saline solutions (HSS) compared with repeated paracentesis and a standard oral diuretic schedule, in patients with cirrhosis and refractory ascites. Methods 59 consecutive cirrhotic patients with refractory ascites unresponsive to outpatient treatment. Enrolled subjects were randomized to treatment with intravenous infusion of furosemide (125-250mg⁄bid) plus small volumes of HSS …

Liver CirrhosisMaleLeptinCirrhosisPhysiologyPeptide Hormonesmedicine.medical_treatmentdiureticlcsh:MedicineVisfatinPathology and Laboratory MedicineFurosemide; Hypertonic Saline Solution; TNF-alpha; IL-1beta; IL-6; ANP; BNP; Visfatin; Leptin; cirrhosis; refractory ascites; paracentesis; diureticBiochemistryGastroenterology0302 clinical medicineRecurrenceFurosemideImmune PhysiologyMedicine and Health SciencesParacentesisDiureticslcsh:ScienceImmune ResponseSalineHypertonicInnate Immune SystemMultidisciplinarymedicine.diagnostic_testLiver DiseasesPhysicsLeptinrefractory asciteAscitesClassical MechanicsFurosemideHematologyMiddle AgedBody FluidsBloodTreatment OutcomeCirrhosis030220 oncology & carcinogenesisPhysical SciencesHypermetabolismCytokinesAdministration IntravenousFemale030211 gastroenterology & hepatologyAnatomyInflammation MediatorsANPResearch ArticleTNF-alphamedicine.drugparacentesimedicine.medical_specialtyInflammatory DiseasesImmunologyGastroenterology and HepatologyBlood Plasma03 medical and health sciencesSigns and SymptomsDiagnostic MedicineOsmotic PressureInternal medicinePressuremedicineTonicityHumansAgedInflammationSaline Solution HypertonicIL-6business.industrylcsh:RBiology and Life SciencesMolecular DevelopmentIL-1betamedicine.diseaseHormonesHypertonic salineEndocrinologyImmune Systemlcsh:QHypertonic Saline SolutionDiureticbusinessBiomarkersDevelopmental BiologyBNPcirrhosi
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Serological Tests Do Not Predict Residual Fibrosis in Hepatitis C Cirrhotics with a Sustained Virological Response to Interferon

2016

BACKGROUND AND AIM: Liver biopsy (LB) has lost popularity to stage liver fibrosis in the era of highly effective anti-hepatitis C virus (HCV) therapy, yet diagnosis of persistent cirrhosis may have important implications following HCV eradication. As performance of serological non-invasive tests (NITs) to predict residual fibrosis in non-viremic HCV patients is unknown, we investigated accuracy of NITs to predict residual fibrosis in cirrhotics after a sustained virological response (SVR) to interferon (IFN). METHODS: Thirty-eight patients with a pre-treatment histological diagnosis of cirrhosis and a 48–104 months post-SVR LB were tested with APRI, CDS, FIB-4, FibroQ, Forns Score, GUCI Ind…

Liver CirrhosisMalePathologyCirrhosisBiopsylcsh:MedicineHepacivirusPathology and Laboratory MedicineGastroenterology0302 clinical medicineEsophageal varicesFibrosisOutcome Assessment Health CareMedicine and Health Scienceslcsh:ScienceSerodiagnosisMultidisciplinarymedicine.diagnostic_testLiver DiseasesHepatitis CMiddle AgedHepatitis C3. Good healthSerologyCirrhosisLiver030220 oncology & carcinogenesisLiver biopsyHepatocellular carcinomaHost-Pathogen InteractionsLiver FibrosisElasticity Imaging Techniques030211 gastroenterology & hepatologyFemaleAnatomyResearch Articlemedicine.medical_specialtyHistologySurgical and Invasive Medical ProceduresGastroenterology and HepatologyAntiviral Agents03 medical and health sciencesDiagnostic MedicineInternal medicineBiopsymedicineHumansSerologic TestsAspartate AminotransferasesAgedbusiness.industryPlatelet Countlcsh:RBiology and Life Sciencesmedicine.diseaseFibrosisROC Curvelcsh:QInterferonsTransient elastographybusinessBiomarkersDevelopmental BiologyPLoS ONE
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Performance of two HCV RNA assays during protease inhibitor-based triple therapy in patients with advanced liver fibrosis and cirrhosis.

2014

Introduction: On-treatment HCV RNA measurements are crucial for the prediction of a sustained virological response (SVR) and to determine treatment futility during protease inhibitor-based triple therapies. In patients with advanced liver disease an accurate risk/benefit calculation based on reliable HCV RNA results can reduce the number of adverse events. However, the different available HCV RNA assays vary in their diagnostic performance. Aim: To investigate the clinical relevance of concordant and discordant results of two HCV RNA assays during triple therapy with boceprevir and telaprevir in patients with advanced liver fibrosis/cirrhosis. Methods: We collected on-treatment samples of 1…

Liver CirrhosisViral DiseasesCirrhosisGastroenterology and hepatologyHepaciviruslcsh:MedicineHepacivirusmedicine.disease_causeGastroenterologyTelaprevirHepatitisLiver diseasechemistry.chemical_compoundMedicinelcsh:ScienceMultidisciplinarybiologyvirus diseasesHepatitis CHepatitis CClinical Laboratory SciencesEuropeClinical LaboratoriesInfectious hepatitisInfectious DiseasesTreatment OutcomeAnti-Retroviral AgentsHCVRNA ViralOligopeptidesmedicine.drugResearch Articlemedicine.medical_specialtyGenotypeProlineHepatitis C virusDiagnostic MedicinePredictive Value of TestsBoceprevirInternal medicineHumansProtease InhibitorsViremiaddc:610Liver diseasesMedicine and health sciencesbusiness.industryClinical Laboratory Techniqueslcsh:RRNAReproducibility of Resultsmedicine.diseasebiology.organism_classificationdigestive system diseaseschemistryImmunologylcsh:Qbusiness
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