Search results for "Digest"
showing 10 items of 3038 documents
Improvement of Analytical Conditions of Mineral Caco-2 Cell Uptake Assays
2004
Caco-2 cell mineral uptake assays are used to estimate the bioavailability of minerals from foods. The uptake of minerals by Caco-2 cells can be affected by several factors – particularly the conditions of the in vitro digestion process and the growth conditions of the cell culture. Therefore, a standardisation of the assays conditions is required to obtain reproducible results. This work determined the effect of enzyme demineralisation, the inactivation of the proteolytic activity of the digest and the replacement of distilled-deionised water by cell culture degree water. Treatment with Chelex-100 resin was applied to remove calcium, iron and zinc from pepsin and pancreatin-bile salts. Th…
Sustainable Valorisation of Biowaste for Soilless Cultivation of Salvia Officinalis in a Circular Bioeconomy
2020
The aim of this work is to assess the usefulness of biowaste deriving from CircularBioeconomy (CBE) processes (i.e., vermicompost, compost and digestate), as growing substratesfor the partial or total replacement of peat, by measuring the vegetation biometric parameters ofsage (Salvia officinalis L.)&mdash
Effect of peracetic acid on levels of geosmin, 2-methylisoborneol, and their potential producers in a recirculating aquaculture system for rearing ra…
2019
Abstract In recirculating aquaculture systems (RAS)s, off-flavors and odors, mainly caused by geosmin (GSM) and 2-methylisoborneol (MIB), can accumulate in the flesh of fish from RAS water, reducing the profitability of production. In this study, peracetic acid (PAA) was applied in three application intervals to pump sumps of rainbow trout (Oncorhynchus mykiss) reared in RAS. Using a real-time polymerase chain reaction (qPCR), the potential off-flavor producers were quantified using geoA and MIB synthase genes. Streptomyces was identified as the major GSM producer, and biofilters showed the highest number of potential off-flavor producers. Concentrations of GSM and MIB were analyzed in the …
Risk of Invasive Lupinus polyphyllus Seed Survival in Biomass Treatment Processes
2021
Invasive plant species threaten native species and habitats causing ecologic, economic and social burden. When creating climate friendly solutions by utilizing plant biomasses in biogas and fertilizer production, safety should be ensured concerning the use of residues. This study concentrates on the treatment of biomasses containing invasive plant material by tunnel and windrow composting, and by farm-scale and laboratory-scale anaerobic digestion (AD) in mesophilic conditions. Germination of the nationally settled and harmful invasive species Lupinus polyphyllus Lindl. was investigated after these processes. In addition, the role of the conditions found in the processes that destroyed seed…
Effect of torrefaction on properties of pellets produced from woody biomass
2020
Torrefaction has been recognized as a promising strategy to improve handling and storage properties of wood-based pellets, thus producing a uniform-quality commodity with high energy density and hydrophobicity. In this work, pellets produced from spruce stem wood, bark, and forest residues were torrefied in a bench-scale tubular reactor at 225 and 275 °C with two residence times (30 and 60 min). The effects of torrefaction on general properties, grindability, mechanical properties, hydrophobicity, and microstructure of the studied pellets were investigated. The increase of torrefaction severity reduced mass yields, but the heating values and the fixed carbon content of the torrefied pellets…
To incinerate or not? - Effects of incineration on the concentrations of heavy metals and leaching efficiency of post-precipitated sewage sludge (RAV…
2020
The major element and heavy metal concentrations of post-precipitated sewage sludge (PPS) and its ash residue (PPA) were determined using microwave digestion followed by inductively coupled plasma optical emission spectrometry (ICP-OES) and mass spectrometry (ICP-MS). To the best of our knowledge, this has not been previously done. In both PPS and PPA the heavy metal concentrations were clearly below the average concentrations than those encountered in sewage sludge in Europe. The leaching efficiency of the metal (Al/ Fe) used as a precipitation agent from post-precipitated sludge and its ash residue with phosphoric acid was also investigated with previously optimized leaching conditions. T…
Analysis of uncontrolled phosphorus precipitation in anaerobic digesters under thermophilic and mesophilic conditions.
2019
This study compares the operation of mesophilic and thermophilic anaerobic digestion of sewage sludge and their effects in uncontrolled phosphorus precipitation. The research has been carried out using a pilot plant consisting of two digesters of 1.6 m3 working volume, treating the mixed sludge of Alzira WWTP (Valencia, Spain). The digesters were operated in parallel, at different conditions: mesophilic (38 ± 2.0°C) and thermophilic (55 ± 2.5°C) temperatures and organic loading rates (OLR) ranging from 1.1 to 1.7 kg volatile solids (VS) m−3 d−1 and different hydraulic retention times (HRT) 20, 15 and 12 days. Uncontrolled precipitation was evaluated through P, Mg and Ca mass balances in bot…
Transformation of Construction Cement to a Self-Healing Hybrid Binder
2019
A new biomimetic strategy to im prove the self-healing properties of Portland cement is presented that is based on the application of the biogenic inorganic polymer polyphosphate (polyP), which is used as a cement admixture. The data show that synthetic linear polyp, with an average chain length of 40, as well as natural long-chain polyP isolated from soil bacteria, has the ability to support self-healing of this construction material. Furthermore, polyP, used as a water-soluble Na-salt, is subject to Na+/Ca2+ exchange by the Ca2+ from the cement, resulting in the formation of a water-rich coacervate when added to the cement surface, especially to the surface of bacteria-containing cement/c…
A method for reclaiming nutrients from aquacultural waste for use in soilless growth systems
2020
Abstract The aim of this work was to develop a method that allows the recovery of nutrients from aquaculture sludge, not only to alleviate the disposal problem, but also to address the future scarcity of non-renewable fertilizers. This method includes two steps: Nutrient mobilization using aerobic digestion followed by solids precipitation using chitosan as the flocculant. The aerobic digestion experiments were conducted in aerated batch reactors, while a jar test apparatus was used to assess the capacity of chitosan to remove total suspended solids (TSS) and turbidity. During aerobic digestion, the concentration of soluble N (sum of NH4-N, NO2-N, NO3-N) increased from 181 mg/L at the start…
Registro y seguimiento clínico de pacientes con síndrome de Peutz Jeghers en Valencia
2020
Resumen: Introducción y objetivos: El síndrome de Peutz Jeghers (SPJ) es una enfermedad rara con herencia autosómica dominante, causada por una mutación germinal del gen STK11/LKB1, localizado en el cromosoma 19p13.3, que consiste en hiperpigmentación mucocutánea, poliposis hamartomatosa y predisposición al cáncer. Se pretende identificar y registrar a los pacientes con SPJ, hacer una descripción de la enfermedad y estimar la prevalencia en Valencia (España). Materiales y métodos: Se obtuvo un listado de historias clínicas en 10 hospitales, utilizando el conjunto mínimo de base de datos de ingresos hospitalarios del Ministerio de Sanidad y Consumo de España utilizando el código CIE-9 759.6.…