Search results for "Digital holographic microscopy"
showing 10 items of 55 documents
Superresolved imaging in digital holography by superposition of tilted wavefronts
2006
A technique based on superresolution by digital holographic microscopic imaging is presented. We used a two dimensional (2-D) vertical-cavity self-emitting laser (VCSEL) array as spherical-wave illumination sources. The method is defined in terms of an incoherent superposition of tilted wavefronts. The tilted spherical wave originating from the 2-D VCSEL elements illuminates the target in transmission mode to obtain a hologram in a Mach-Zehnder interferometer configuration. Superresolved images of the input object above the common lens diffraction limit are generated by sequential recording of the individual holograms and numerical reconstruction of the image with the extended spatial frequ…
Optically-undistorted digital holographic microscopy for quantitative phase-contrast imaging
2011
We propose a telecentric architecture for circumventing, by a pure-optical method, the residual phase distortion inherent to standard configuration of digital holographic microscopy (DHM). With this proposal there is no need for computer compensation of the parabolic phase during the phase map recovering procedure. Futhermore, in off-axis configuration, the spatial frequency useful domain is enlarged. The validity of the method is demonstrated by performing quantitative measurements of depth differences
Resolution improvement by single-exposure superresolved interferometric microscopy with a monochrome sensor
2011
Single-exposure superresolved interferometric microscopy (SESRIM) by RGB multiplexing has recently been proposed as a way to achieve one-dimensional superresolved imaging in digital holographic microscopy by a single-color CCD snapshot [Opt. Lett. 36, 885 (2011)]. Here we provide the mathematical basis for the operating principle of SESRIM, while we also present a different experimental configuration where the color CCD camera is replaced by a monochrome (B&W) CCD camera. To maintain the single-exposure working principle, the object field of view (FOV) is restricted and the holographic recording is based on image-plane wavelength-dispersion spatial multiplexing to separately record the thre…
Enhancing spatial resolution in digital holographic microscopy by biprism structured illumination.
2014
A novel and efficient architecture of a structured-illumination digital holographic microscope (DHM) is presented. As the DHM operates at the diffraction limit, its spatial resolution on label-free imaging of transparent samples is improved by illuminating the sample with a structured illumination produced by a Fresnel's biprism. The theoretical analysis of the method forecasts a twofold improvement of the spatial resolution. The proposed method requires only two images to improve the spatial resolution, which eases the process of unmixing the high-resolution components by means of an unknown phase-shift procedure. Numerical modeling and experimental results validate the theoretical finding…
Compact and Field Portable Biophotonic Sensors for Automated Cell Identification (Plenary Address)
2021
In this Plenary address paper, we overview recently published work for automated cell identification using 3D optical imaging in compact and field portable biophotonic sensors. Digital holographic microscopy systems and lensless pseudorandom phase encoding systems capture 3D information of biological cells and make highly accurate automated cell identification possible. Overviewed systems include sickle cell disease diagnosis based on spatio-temporal cell dynamics in a field-portable 3D-printed shearing digital holography as well as lensless cell identification of both single and multicell samples using pseudorandom phase encoding.
Resolution Enhancement in Phase Microscopy: a Review
2018
Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3-D topography in reflective samples as well as the inner structure or refractive index distribution of transparent and translucent samples. However, as in conventional optical microscopy, QPM provides either a large field of view (FOV) or a high resolution but not both. Many approaches such as oblique illumination, structured illumination and speckle illumination have been proposed to improve the spatial resolution of phase microscopy by restricting other degrees of freedom (mostly time). Therefore, the space bandwidth product (SBP) of QPM becomes enlarged. This paper aims …
Versatile optimization-based speed-up method for autofocusing in digital holographic microscopy
2021
We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations to decide the in-focus position. The classical autofocusing algorithms apply a uniform search strategy, i.e., they probe multiple, uniformly distributed axial locations, which leads to heavy computational overhead. Our method substantially reduces the computational load, without sacrificing the accuracy, by skillfully selecting the next location to investigate, which results in a decreased total number of probed propagation distances. This is achieved by…
Characterization of the Microshell Surface Using Holography
2011
AbstractTo characterize the shape, the quality, and the roughness of microshells, digital holographic microscopy technology is used because it offers an appropriate ability to these studies. It captures holograms to reconstruct a double image, one for the intensity and another one for the phase. Using rotation axis, bump counting for the complete microshell surface is possible with a very high speed. Using image stitching and three-dimensional surface rebuilding software, mapping can be done in a few minutes. Each bump can then be characterized on the map by its position, diameter, and height.
Axial superresolution by synthetic aperture generation
2008
The use of tilted illumination onto the input object in combination with time multiplexing is a useful technique to overcome the Abbe diffraction limit in imaging systems. It is based on the generation of an expanded synthetic aperture that improves the cutoff frequency (and thus the resolution limit) of the imaging system. In this paper we present an experimental validation of the fact that the generation of a synthetic aperture improves not only the lateral resolution but also the axial one. Thus, it is possible to achieve higher optical sectioning of three-dimensional (3D) objects than that defined by the theoretical resolution limit imposed by diffraction. Experimental results are provi…
Digital Holographic Microscopy: A New Imaging Technique to Quantitatively Explore Cell Dynamics with Nanometer Sensitivity
2014
In the first part of this chapter, we describe how the new concept of digital optics applied to the field of holographic microscopy has made it possible to quantitatively and accurately measure the phase retardation induced on the transmitted wavefront by the observed transparent specimen, allowing thus to develop a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM). In the second part the most relevant DH-QPM applications in the field of cell biology are presented. Particularly, applications taking directly advantage of benefits provided by digital optics particularly off-line autofocusing and extended depth of focus, are outlined. Otherwise, special emphasis …