Search results for "Digital"
showing 10 items of 3267 documents
Wavelength-compensated time-sequential multiplexed color joint transform correlator
2010
We report a wavelength-compensated three-channel (RGB) joint transform correlator (JTC) for color pattern recognition using a ferroelectric liquid-crystal spatial light modulator (SLM) operating in binary pure phase modulation. We apply a previously reported time-multiplexing technique useful in creating wavelength-compensated diffraction patterns, based on the synchronization of properly scaled diffraction masks with the input wavelength selection obtained by applying a rotating RGB color-filter wheel to an Ar-Kr laser. The application of this technique to a JTC architecture permits real-time color object detection. In order to achieve a high light efficiency for the correlation process, w…
Coherent Microscopy for 3-D Movement Monitoring and Super-Resolved Imaging
2010
In this chapter we present three types of microscopy-related configurations while the first one is used for 3-D movement monitoring of the inspected samples, the second one is used for super-resolved 3-D imaging, and the last one presents an overview digital holographic microscopy applications. The first configuration is based on temporal tracking of secondary reflected speckles when imaged by properly defocused optics. We validate the proposed scheme by using it to monitor 3-D spontaneous contraction of rat’s cardiac muscle cells while allowing nanometric tracking accuracy without interferometric recording. The second configuration includes projection of temporally varying speckle patterns…
Study of spatial lateral resolution in off-axis digital holographic microscopy
2015
The lateral resolution in digital holographic microscopy (DHM) has been widely studied in terms of both recording and reconstruction parameters. Although it is understood that once the digital hologram is recorded the physical resolution is fixed according to the diffraction theory and the pixel density, still some researches link the resolution of the reconstructed wavefield with the recording distance as well as with the zero-padding technique. Aiming to help avoiding these misconceptions, in this paper we analyze the lateral resolution of DHM through the variation of those two parameters. To support our outcomes, we have designed numerical simulations and experimental verifications. Both…
Fast and robust phase-shift estimation in two-dimensional structured illumination microscopy.
2019
A method of determining unknown phase-shifts between elementary images in two-dimensional Structured Illumination Microscopy (2D-SIM) is presented. The proposed method is based on the comparison of the peak intensity of spectral components. These components correspond to the inherent structured illumination spectral content and the residual compo- nent that appears from wrongly estimated phase-shifts. The estimation of the phase-shifts is carried out by finding the absolute maximum of a function defined as the normalized peak intensity difference in the Fourier domain. This task is performed by an optimization method providing a fast estimation of the phase-shift. The algorithm stability an…
Superresolved Holographic Microscopy
2008
Superresolution methods in digital holographic microscopy provide a useful tool to overcome the Abbe's diffraction limit when using modest microscope lenses. The process improves the cutoff frequency of the microscope lens by means of the generation of a synthetic aperture based on time multiplexing and using 3 main stages: optical coding, optical decoding, and digital postprocessing. After the whole process, a superresolved image is obtained by Fourier transformation of the synthetic aperture.
Superesolution in digital holographic microscopy
2011
In this contribution, we address with the possibility to overcome the limited resolving power of imaging systems beyond the limit imposed by Abbe's diffraction theory. We first review the mathematical foundations underlying superresolution (SR) from an information theory point of view and then we focus on two multiplexing approaches in digital holographic microscopy (DHM) for achieving SR by synthetic aperture (SA) generation.
Synthetic Aperture Lensless Digital Holographic Microscopy (SALDHM) for Superresolved Biological Imaging
2013
Lensless digital holographic microscopy (LDHM) relates with the capability to achieve microscopic imaging working without lenses in the regime of holography. LDHM uses an extremely simplified setup and provides micron-range lateral and depth resolutions in three-dimensional (3D) imaging. Typically, LDHM uses a pinhole to provide spherical divergent illumination over the sample. Then, two different basic schemes are usually adopted for providing holographic recording. The first one assumes the interference between two in-line waves since diffraction by the sample can be considered as a perturbation of the reference wave. The second scheme uses an external reference beam in an off-line config…
Superresolved phase-shifting Gabor holography by CCD shift
2009
Holography in the Gabor regime is restricted to weak diffraction assumptions. Otherwise, diffraction prevents an accurate recovery of the object's complex wavefront. We have recently proposed a modified Gabor-like setup to extend Gabor's concept to any sample provided that it be non-diffusive. However, the resolution of the final image becomes limited as a consequence of the additional elements considered in the proposed setup. In this paper we present an experimental approach to overcome such a limitation in which the former configuration is used while the CCD camera is shifted to different off-axis positions in order to generate a synthetic aperture. Thus, once the whole image set is reco…
Transverse resolution improvement using rotating-grating time-multiplexing approach
2008
The ability to improve the limited resolving power of optical imaging systems while approaching the theoretical diffraction limit has been an attractive discipline with growing interest over the last years due to its benefits in many applied optics systems. This paper presents a new approach to achieve transverse superresolution in far-field imaging systems, with direct application in both digital microscopy and digital holographic microscopy. Theoretical analysis and computer simulations show the validity of the presented approach.
Il dialogo tra Capability Approach e la form-azione delle competenze digitali
2020
Il presente contributo si focalizza su una sfida educativa impellente della contemporaneità: la form-azione della cittadinanza digitale. A partire dal Quadro di riferimento Europeo per le competenze digitali dei cittadini, viene svolta una riflessione basata sull’interconnessione tra il Capability approach e le competenze digitali. Il Capability Approach, sviluppando un orizzonte di senso eticoeconomico-politico basato su: “functioning”, capability” e “agency”, completa e orienta il framework teorico-metodologico dettato dai riferimenti normativi europei sullo sviluppo delle digital skills. L’indagine sulle digital skills può essere un’opportunità per orientare l’azione formativa, in riferi…