Search results for "Dinitrophenol"

Calcium transport in rat small intestine in vitro and in vivo

1972

Intestinal calcium (Ca) transport was studied in the rat jejunum by the in vitro perfusion technique of Fisher and Parsons and in the tied loop in vivo. Mucosal uptake and absorption of Ca was examined under the following conditions: rising intraluminal Ca-concentrations (0.5–128 meq/l); inhibition of energy dependent metabolism (2,4-dinitrophenol, N2, low temperature); net water flow, out of or into the intestinal lumen; addition of strontium (Sr); pretreatment with low Ca-diet and with 6-methyl-prednisolone. The concentration-dependent Ca absorption curve rose steeply at low Ca-concentrations but changed to a slowly rising straight line above 16 meq/l Ca++. In contrast, Ca uptake into the…

In activated mast cells, IL-1 up-regulates the production of several Th2-related cytokines including IL-9.

2000

Abstract Mast cells can play detrimental roles in the pathophysiology and mortality observed in anaphylaxis and other Th2-dominated allergic diseases. In contrast, these cells contribute to protective host defense mechanisms against parasitic worm infections. After IgE/Ag activation, mast cells can produce multiple cytokines that may enhance allergic inflammations, while a similar panel of Th2-related cytokines may support immunological strategies against parasites. Here we report that in primary mouse bone marrow-derived mast cells activated by ionomycin or IgE/Ag, the proinflammatory mediator IL-1 (α or β) up-regulated production of IL-3, IL-5, IL-6, and IL-9 as well as TNF, i.e., cytokin…

Metabolic aspects of the rhythmogenesis inAplysia pacemaker neurons

1973

Completely isolatedAplysia pacemaker neurons were used to investigate mechanisms of endogenous electrical rhythmicity. This preparation allows the study of pure pacemaker activity free from synaptic, ephaptic and/or humoral influences from the surrounding cells. The effect of some substances quite different in their mode of biochemical action were tested: sodium iodoacetate, phloridzin, dinitrophenol, heavy water, and ouabain. Each of these substances suppressed the spontaneous spike activity without any marked depolarizing or hyperpolarizing effect. Spontaneous spike activity of the neurons silenced after addition of one of these substances reappeared after addition of glucose. The restori…

Die positiv inotrope Wirkung von Theophyllin, Adrenalin und Strophanthin am Papillarmuskel bei Sch�digung durch Dinitrophenol, Azid, Chinin und Barbi…

1960

Inhibition of FcεRI-mediated Activation of Rat Basophilic Leukemia Cells by Clostridium difficile Toxin B (Monoglucosyltransferase)

1996

Abstract Treatment of rat basophilic leukemia (RBL) 2H3-hm1 cells with Clostridium difficile toxin B (2 ng/ml), which reportedly depolymerizes the actin cytoskeleton, blocked [3H]serotonin release induced by 2,4-dinitrophenyl-bovine serum albumin, carbachol, mastoparan, and reduced ionophore A23187-stimulated degranulation by about 55-60%. In lysates of RBL cells, toxin B 14C-glucosylated two major and one minor protein. By using two-dimensional gel electrophoresis and immunoblotting, RhoA and Cdc42 were identified as protein substrates of toxin B. In contrast to toxin B, Clostridium botulinum transferase C3 that selectively inactivates RhoA by ADP-ribosylation did not inhibit degranulation…