Search results for "Domu"
showing 10 items of 167 documents
Differentiation capacity of epithelial cells in the sponge Suberites domuncula.
2004
Sponges (phylum Porifera) represent the oldest metazoans. Their characteristic metazoan adhesion molecules and transcription factors enable them to establish a complex "Bauplan" ; three major differentiated cell types (epithelial cells, skeletal cells/sclerocytes, and contractile cells) can be distinguished. Since no molecular markers are as yet available to distinguish these somatic cells or the corresponding embryonic cells from which they originate, we have selected the following three genes for their characterization: noggin (a signaling molecule in development), a caspase that encodes an apoptotic molecule, and silicatein. Silicatein is an enzyme that is involved in the synthesis of si…
Histochemical and electron microscopic analysis of spiculogenesis in the demosponge Suberites domuncula.
2006
The skeleton of demosponges is built of spicules consisting of biosilica. Using the primmorph system from Suberites domuncula, we demonstrate that silicatein, the biosilica-synthesizing enzyme, and silicase, the catabolic enzyme, are colocalized at the surface of growing spicules as well as in the axial filament located in the axial canal. It is assumed that these two enzymes are responsible for the deposition of biosilica. In search of additional potential structural molecules that might guide the mineralization process during spiculogenesis to species-specific spicules, electron microscopic studies with antibodies against galectin and silicatein were performed. These studies showed that …
Genome size and chromosomes in marine sponges [Suberites domuncula, Geodia cydonium]
1995
The genome size of the marine sponges Suberites domuncula and Geodia cydonium has been determined by flow cytofluorometric analysis using diamidino-phenylindole [DAPI]. Using human lymphocytes as reference the amount of DNA in cells from S. domuncula has been determined to be 3.7 pg and that of G. cydonium 3.3 pg. While no chromosomes could be identified in G. cydonium, the karyotype of the Suberites domuncula is 32 chromosomes in the diploid state. The size of the chromosomes was between 0.25 and 1.0 micron. No pronounced banding pattern was visible.
Nota a 1) Cass. 23 gennaio 2012, n.11621; 2) Cass. 16 dicembre 2011, n. 9907; 3)Cass. 5 dicembre 2011, n. 15026.
2013
Nota a tre sentenze della Cassazione in materia di ingiuria e minacce. Più in particolare, sub 1) rilevanza penale dell'espressione "lei non sa chi sono io"; sub 2) presupposti e limiti dell'esimente della provocazione; sub 3) rilevanza dell'ingiuria da parte del soggetto sordomuto.
Innate Immune Defense of the Sponge Suberites domuncula against Bacteria Involves a MyD88-dependent Signaling Pathway
2005
Sponges (phylum Porifera) are the phylogenetically oldest metazoa; as filter feeders, they are abundantly exposed to marine microorganisms. Here we present data indicating that the demosponge Suberites domuncula is provided with a recognition system for Gram-negative bacteria. The lipopolysaccharide (LPS)-interacting protein was identified as a receptor on the sponge cell surface, which recognizes the bacterial endotoxin LPS. The cDNA was isolated, and the protein (Mr 49,937) was expressed. During binding to LPS, the protein dimerizes and interacts with MyD88, which was also identified and cloned. The sponge MyD88 (Mr 28,441) is composed of two protein interaction domains, a Toll/interleuki…
On the origin of Metazoan adhesion receptors: cloning of integrin alpha subunit from the sponge Geodia cydonium
1997
Integrins are prominent receptors known from vertebrates and the higher phyla of invertebrates. Until now, no evidence has been provided for the existence of integrins in the lowest Metazoa, the sponges (Porifera). We have isolated and characterized a cDNA clone encoding the alpha subunit of integrin from the marine sponge Geodia cydonium (GCINTEG). The open reading frame encodes a polypeptide of 1,086 residues (118 kDa). The intracellular domain features the sequence Tyr-Phe-x-Gly-Phe-Phe-x-Arg, which is different in one residue from the characteristic consensus pattern for integrin alpha subunits. We conclude that sponges, the oldest multicellular animal phylum, already utilize the struct…
Increased gene expression of a cytokine-related molecule and profilin after activation of Suberites domuncula cells with xenogeneic sponge molecule(s)
2000
Porifera (sponges) constitute the lowest metazoan phylum, Experiments examined whether sponges can recognize self/nonself molecules. Cells from the marine sponge Suberites domuncula were incubated with membranes from either S. domuncula or another marine sponge, Geodia cydonium, as well as with recombinant alpha-integrin from G. cydonium. The cells responded immediately with a rise of intracellular Ca2+ ([Ca-i(2+)]) if they were treated with membranes from G. cydonium but not after treatment by those from S. domuncula. This change of [Ca-i(2+)] was also recorded with G. cydonium alpha-integrin. In parallel, the expression of two genes was strongly upregulated; one codes for a cytokine-relat…
Cultivation of primmorphs from the marine sponge Suberites domuncula: morphogenetic potential of silicon and iron.
2002
Abstract Marine demosponges (phylum Porifera) are rich sources for potent bioactive compounds. With the establishment of the primmorph system from sponges, especially from Suberites domuncula , the technology to cultivate sponge cells in vitro improved considerably. This progress was possible after the elucidation that sponges are provided with characteristic metazoan cell adhesion receptors and extracellular matrix molecules which allow their cells a positioning in a complex organization pattern. This review summarizes recent data on the cultivation of sponges in aquaria and—with main emphasis—of primmorphs in vitro. It is outlined that silicon and Fe(+++) contribute substantially to the f…
Rīta aplis kā mācību organizācijas veids 4 gadīgiem bērniem
2022
Kursa darba tēma: Rīta aplis, kā mācību darba organizācijas veids 4 gadīgiem bērniem. Darba autors: Māra Pērkone Darba vadītāja: Dr. paed Ieva Margēviča-Grinberga Darba apjoms: 49. lpp., 26 literatūras un avotu vienības, 4 pielikumi Atslēgas vārdi: Izglītība, bērna attīstība, rīta aplis, domu kartes, runājošā siena. Kursa darbā veikts teorētisks pētījums un literatūras analīze par akcentu un vērtību maiņu izglītības sistēmā, gan Latvijas, gan pasaules mērogā. Tika pētīti literatūras avoti, kas apstiprināja “rīta apli”, kā svarīgu dienas darba sastāvdaļu. Literatūrā tika meklēti apstiprinājumi darba metodēm, kuras izmantot rīta aplī, metodēm, kuras viss labāk motivē bērnus radošai darbībai u…
Zvaigžņotā Debess: 2000, Pavasaris (167)
2000
Contents: P.Müürsepp. B.Schmidt, Prominent Optician of 20th Century ; J.Miezis. Buildings on the Moon ; Z.Alksne, A.Alksnis. Stars Enshrouded in Envelopes ; A.Alksnis. Goals and Progress of the DENIS Program ; Z.Alksne. Shapley Supercluster – Playground of Clusters of Galaxies ; A.Alksnis. Wide Field Imager Discovers Distant Galaxies ; J.Jaunbergs. Roton SSTO: First Flight Tests ; M.Gills. Space Research 40 Years Ago – Chronicle ; I.Vilks. The Secret of Low-Probability Complexity in the Universe ; K.Bērziņš. On Friendly Terms with Cosmology: Development of Cosmological Views (concluded) ; A.Balklavs. Astronomy in the Internet (WWW) ; V.Flerov, A.Cēbers. Latvia 23rd Open Olympiad of Physics:…