Search results for "Dono"
showing 10 items of 572 documents
Evidencias para la reducción del abandono educativo
2022
Este texto expone la determinación del abandono educativo, aporta las series de datos europeos y relaciona el fenómeno con otras variables educativas y sociales. Concluye que la correlación estadística más importante se establece con la desigualdad social, por lo que la reducción del abandono no puede reducirse a actuaciones pedagógicas o didácticas. This text exposes the determination of educational dropout, provides the European data series and relates the phenomenon with other educational and social variables. It concludes that the most important statistical correlation is established with social inequality, so the dropout reduction cannot be reduced to pedagogical or didactic actions.
Improving mRNA-Based Therapeutic Gene Delivery by Expression-Augmenting 3' UTRs Identified by Cellular Library Screening.
2019
Synthetic mRNA has emerged as a powerful tool for the transfer of genetic information, and it is being explored for a variety of therapeutic applications. Many of these applications require prolonged intracellular persistence of mRNA to improve bioavailability of the encoded protein. mRNA molecules are intrinsically unstable and their intracellular kinetics depend on the UTRs embracing the coding sequence, in particular the 3′ UTR elements. We describe here a novel and generally applicable cell-based selection process for the identification of 3′ UTRs that augment the expression of proteins encoded by synthetic mRNA. Moreover, we show, for two applications of mRNA therapeutics, namely, (1) …
Nitric oxide increases the decay of matrix metalloproteinase 9 mRNA by inhibiting the expression of mRNA-stabilizing factor HuR.
2003
Dysregulation of extracellular matrix turnover is an important feature of many inflammatory processes. Rat renal mesangial cells express high levels of matrix metalloproteinase 9 (MMP-9) in response to inflammatory cytokines such as interleukin-1 beta. We demonstrate that NO does strongly destabilize MMP-9 mRNA, since different luciferase reporter gene constructs containing the MMP-9 3' untranslated region (UTR) displayed significant reduced luciferase activity in response to the presence of NO. Moreover, by use of an in vitro degradation assay we found that the cytoplasmic fractions of NO-treated cells contained a higher capacity to degrade MMP-9 transcripts than those obtained from contro…
Valsts asinsdonoru centra personāla skatījums uz ziedotajām asinīm
2017
Bakalaura darba tēma ir „Valsts asinsdonoru centra personāla skatījums uz ziedotajām asinīm”. Konkrētais pētījums piedāvā informāciju par Valsts asindonoru centra pieejamību publiskajā telpā, kā arī, balstoties uz empīriskajiem datiem, norāda par medicīnas personāla praksēm, kas saistītas ar ziedošanas procesu. Pētījums sastāv no teorētiskā apraksta, mediju analīzes, līdzdalīgā novērojuma un autora refleksijas par darba attīstības gaitu. Iepriekšminētās metodes tika izmantotas kā galvenais rīks, lai atbildētu uz pētījumā izvirzīto mērķi, uzdevumiem un jautājumiem. Pētījuma rezultāti norāda, ka medicīnas personāla loma ir nozīmīga, tas attiecas arī uz ar to saistītajiem noteikumiem (asiņu ka…
Zvaguļu (Rhinanthus) izmantošana zālāju ekoloģiskajā atjaunošanā auglīgās augsnēs: sēklu kvalitātes un konkurences nozīme
2022
Ir zināms, ka mūsdienās viena no globālām problēmām ir klimata pārmaiņas, kura skar arī dažādas ekosistēmas un to stāvokli. Zālāju ekosistēmas ir diezgan jūtīgas pret apkārtējās vides izmaiņām, līdz ar to Latvijā ir novērojama dabisko zālāju sarukšanas tendence. Tādēļ, dabiskos zālājus ir nepieciešams saglābāt, apsaimniekot un atjaunot Latvijas mērogā. Ekoloģiskās atjaunošanas metodes mēdz būt visai dažādas, taču viena no jaunākajām, līdz šim Latvijā neaprobētām metodēm, ir zvaguļu pusparazītaugu ieviešanas metode, kura paredzēta zālājā esošo sugu konkurences samazināšanai, un maģistra darba mērķis ir noskaidrot sēklu kvalitātes un veģetācijas konkurences nozīmi zvaguļu izmantošanā zālāju e…
Association of vitamin D serum levels and its common genetic determinants, with severity of liver fibrosis in genotype 1 chronic hepatitis C patients.
2013
Background and aims: Lower 25-Hydroxyvitamin D (25[OH]D) serum lev- els have been associated with the severity of liver fibrosis in genotype 1 chronic hepatitis C patients (G1CHC). In addition, a recent genome-wide study identified genetic variants (rs12785878, near dehydrocholesterol reduc- tase, DHCR7; rs10741657, near CYP2R1; and rs7041, near vitamin D binding protein, GC) affecting 25(OH)D serum levels in healthy populations. We aimed to assess the association between vitamin D serum levels and its genetic determinants, with the severity of liver fibrosis. Material and methods: Two hundred sixty patients with biopsy-proven G1CHC were consecutively evaluated. The 25(OH)D serum levels wer…
Diagnostic follow-up of blood donors with indeterminate results to Western Blot for HIV infection in Palermo, Sicily
2002
27,343 blood donors, were tested for HIV infection at the Poli- clinico of Palermo in the period 1999-2001 by using a com- mercial ELISA as screening test and a commercial Western Blot (WB) as confirmatory test. HIV infection was detected in two subjects, whereas five individuals reacted faintly at the screening and tested indeterminate at the confirmatory test showing the presence of only one band for gp41 (four cases) or p24 (one case). A one year follow-up was performed in these patients. All donors but one, spontaneously seroreverted during the follow-up; the only one who persisted as WB inde- terminate, tested also negative for HIV RNA by PCR assay at the end of follow-up. In conclusio…
Selective Stepwise Suzuki Cross-Coupling Reaction for the Modelling of Photosynthetic Donor−Acceptor Systems
2009
A Suzuki reaction performed as a selective stepwise substitution of two boryl groups on a diarylporphyrin precursor is reported for straightforward construction of a porphyrin trimer, modeling photosynthetic donor-acceptor systems.
Alien flora of Europe: Species diversity, temporal trends, geographical patterns and research needs
2008
International audience; The paper provides the first estimate of the composition and structure of alien plants occurring in the wild in the European continent, based on the results of the DAISIE project (2004–2008), funded by the 6th Framework Programme of the European Union and aimed at “creating an inventory of invasive species that threaten European terrestrial, freshwater and marine environments”. The plant section of the DAISIE database is based on national checklists from 48 European countries/regions and Israel; for many of them the data were compiled during the project and for some countries DAISIE collected the first comprehensive checklists of alien species, based on primary data …
Increased electron donor and electron acceptor characters enhance the adhesion between oil droplets and cells of Yarrowia lipolytica as evaluated by …
2003
International audience; The adhesion of methyl ricinoleate droplets to cells of the yeast Yarrowia lipolytica was investigated. A new cytometric method, relying on the double staining of fatty globules with Nile Red and of cells with Calcofluor, enabled us to quantify methyl ricinoleate droplet adhesion to cells precultured on a hydrophilic or on a hydrophobic carbon source. In this last case, droplet adsorption was enhanced and a MATS (microbial adhesion to solvents) test revealed that this increase was due to Lewis acid-base interactions and not to an increase in the hydrophobic properties of the cell surface. These preliminary results demonstrate that the developed cytometric method is p…