Search results for "Dpp"
showing 10 items of 168 documents
Potential in vitro antioxidant, anti-inflammatory, antidiabetic, and anticancer effect of arachidonic acid-elicited basil leaves
2017
Abstract The study presents the impact of elicitation of basil with 0.01 µM (AA1), 1 µM (AA2) and 100 µM (AA3) arachidonic acid on the content and bioactivity of potentially bioavailable polyphenols. Elevated levels of phenolic compounds correlated with increased biological activity were observed in the extracts from basil (control and elicited) subjected to simulated gastrointestinal digestion in comparison to chemical and standard buffer extracts. Elicitation with AA1 and AA2 resulted in an 3-fold and more than 4-fold increase in the content of potentially bioavailable rosmarinic acid, respectively. In some cases, elicitation with arachidonic acid improved the pro-health properties of the…
Comparative analysis of radical scavenging and antioxidant activity of phenolic compounds present in everyday use spice plants by means of spectropho…
2010
Comparative analysis of radical scavenging and antioxidant activities of phenolic compounds present in everyday use spice plants was carried out by means of spectrophotometric and chromatographic methods. Six spice plant samples, namely onion (Allium cepa), parsley (Petroselinum crispum) roots and leaves, celery (Apium graveolens) roots and leaves and leaves of dill (Anethum graveolens) were analyzed. Total amount of phenolic compounds and radical scavenging activity (RSA) was the highest in celery leaves and dill extracts and was the lowest in celery roots. Comparing commonly used spectrophotometric analysis of 2,2-diphenyl-1-picrylhydrazyl (DPPH) RSA of extracts with the results obtained …
Metabolite profile and in vitro activities of Phagnalon saxatile (L.) Cass. relevant to treatment of Alzheimer’s disease
2009
The present study describes for the first time the in vitro properties (inhibition of NO production and anticholinesterase) of Phagnalon saxatile (L.) Cass. (Asteraceae). The methanolic extract showed antioxidant activity that was measured by DPPH assay and beta-carotene bleaching test. The same extract inhibited NO production in the murine monocytic macrophage cell line RAW 264.7. Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibition was assessed by modifications of Ellman's method. Purification of the MeOH extract of P. saxatile allowed the isolation of phenolic compounds. Among them, the compounds that most effectively inhibited lipopolysaccharide-induced NO production …
Phytochemical profile and antioxidative properties of Plinia trunciflora fruits: A new source of nutraceuticals.
2020
Abstract This study evaluated the polyphenol profile and the antioxidative properties of Plinia trunciflora (O. Berg) Kausel fruits. Folin-Ciocalteau and pH-jumping methods indicated that these berries are a major source of antioxidant polyphenols (1201.05 mg GAE/100 g FW), particularly anthocyanins. HPLC-DAD-ESI-MS/MS analysis identified cyanidine glycosides as the main components. Flavon-3-ols and hydrolysable-tannins were also found. CAA assay showed that extracts of P. trunciflora fruits prevent lipid peroxidation in HepG2 cells with higher efficacy than other colourful fruits (CAA50 935.25 mg FW/mL cell medium). Moreover, our results suggested that the observed antioxidant protection i…
The Antioxidant Potential of White Wines Relies on the Chemistry of Sulfur-Containing Compounds: An Optimized DPPH Assay
2019
The DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay is an easy and efficient method commonly used to determine the antioxidant capacity of many food matrices and beverages. In contrast with red wines, white wines are poorer in antioxidant polyphenolics, and the more hydrophilic sulfur-containing compounds in them may contribute significantly to their antioxidant capacity. The modification of the classical DPPH method, with a methanol-buffer and the measure of EC20 (quantity of sample needed to decrease the initial DPPH concentration by 20%) has shown that sulfur-containing compounds such as cysteine (0.037 ±
Antioxidant and neuroprotective effects of synthesized sintenin derivatives
2009
Three series of sintenin derivatives (compounds 1-14) were designed and prepared and their antioxidative and neuroprotective effects were evaluated. The in vitro models of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, chelating ferrous ions, inhibiting the rat brain homogenates lipid peroxidation, and protecting neurons damaged by hydrogen peroxide were employed for bioassays. It was found that sintenin derivatives 4 and 13 showed remarkable antioxidative and neuroprotective activities.
In Vitro Phytotoxicity and Antioxidant Activity of Selected Flavonoids
2012
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Influence of enzyme-aided extraction and ultrasonication on the phenolics content and antioxidant activity of Paeonia officinalis L. petals
2020
Peony is a less utilized herbaceous plant of ornamental and medicinal relevance. The petals are rich in bioactive phenolics and hence, the development of efficient extraction is required for maximum recovery and bioactivity. In this study, the optimal conditions for the ultrasound-assisted extraction of peony phenolics were first investigated by comparing to conventional extraction and testing the data fit by mathematical models (linear, two factor interactions, quadratic) followed by investigation of cellulase pre-treatment combined with ultrasonication. High amounts of flavonoids, phenolics and tannins were extracted in 30 min under ultrasound conditions of 150 W, 40 kHz, 50 % amplitude, …
Role of paramagnetic polyconjugated clusters in lignin antioxidant activity(in vitro)
2012
Using physico-chemical methods (EPR, SEC, Py-GC/MS and UV/VIS spectroscopy) and wet chemical analysis, the characteristics of 6 hardwood lignins in terms of functionality, molecular weight and composition of lignin substructures were determined and considered together with the results of DPPH•, ABTS•+ and O2•− antioxidant assays with the aim to understand the relationships governing antioxidant properties of lignin. The strong positive linear correlation between lignin antioxidant capacity in the three assays used and the extent of conjugation of paramagnetic polyconjugated clusters in lignin macromolecules was found. The biological activity of the most active alkaline lignins was assessed …
Polyphenol Characterization, Antioxidant and Skin Whitening Properties of Alnus cordata Stem Bark
2019
In this study, we investigated the phenolic composition of the crude extract (MeOH 80 %) of Alnus cordata (Loisel.) Duby stem bark (ACE) and its antioxidant and skin whitening properties. RP-LC-DAD analysis showed a high content of hydroxycinnamic acids (47.64 %), flavanones (26.74 %) and diarylheptanoids (17.69 %). Furthermore, ACE exhibited a dose-dependent antioxidant and free-radical scavenging activity, expressed as half-maximal inhibitory concentration (IC50 ): Oxygen radical absorbance capacity (ORAC, IC50 1.78 μg mL-1 )>Trolox equivalent antioxidant capacity (TEAC, IC50 3.47 μg mL-1 )>2,2-Diphenyl-1-picrylhydrazyl (DPPH, IC50 5.83 μg mL-1 )>β-carotene bleaching (IC50 11.58 μg mL-1 )…