Search results for "EMC"
showing 10 items of 369 documents
Gemcitabine sensitizes lung cancer cells to Fas/FasL system-mediated killing
2014
Gemcitabine is a chemotherapy agent commonly used in the treatment of non-small cell lung cancer (NSCLC) that has been demonstrated to induce apoptosis in NSCLC cells by increasing functionally active Fas expression. The aim of this study was to evaluate the Fas/Fas ligand (FasL) system involvement in gemcitabine-induced lung cancer cell killing. NSCLC H292 cells were cultured in the presence or absence of gemcitabine. FasL mRNA and protein were evaluated by real-time PCR, and by Western blot and flow cytometry, respectively. Apoptosis of FasL-expressing cells was evaluated by flow cytometry, and caspase-8 and caspase-3 activation by Western blot and a colorimetric assay. Cytotoxicity of ly…
Sphingomonas fennica sp. nov. and Sphingomonas haloaromaticamans sp. nov., outliers of the genus Sphingomonas.
2007
Bacterial isolates obtained from polychlorophenol-contaminated sites in Finland (strain K101T) and from a Dutch drinking water well (strain A175T) were characterized taxonomically. 16S rRNA gene sequence analysis, determination of DNA G+C content, physiological characterization, estimation of the ubiquinone and polar lipid patterns and fatty acid content revealed that strains K101T and A175T were similar to Sphingomonas wittichii RW1T but also showed pronounced differences. The DNA G+C contents of the two novel strains were 63.6 and 66.1 mol%, respectively. On the basis of these results, two novel species of the genus Sphingomonas are described, for which the names Sphingomonas haloaromatic…
An Efficient Algorithm for the Generation of Z-Convex Polyominoes
2014
We present a characterization of Z-convex polyominoes in terms of pairs of suitable integer vectors. This lets us design an algorithm which generates all Z-convex polyominoes of size n in constant amortized time.
Approximate convex hull of affine iterated function system attractors
2012
International audience; In this paper, we present an algorithm to construct an approximate convex hull of the attractors of an affine iterated function system (IFS). We construct a sequence of convex hull approximations for any required precision using the self-similarity property of the attractor in order to optimize calculations. Due to the affine properties of IFS transformations, the number of points considered in the construction is reduced. The time complexity of our algorithm is a linear function of the number of iterations and the number of points in the output convex hull. The number of iterations and the execution time increases logarithmically with increasing accuracy. In additio…
The computational complexity of the relative robust shortest path problem with interval data
2004
Abstract The paper deals with the relative robust shortest path problem in a directed arc weighted graph, where arc lengths are specified as intervals containing possible realizations of arc lengths. The complexity status of this problem has been unknown in the literature. We show that the problem is NP -hard.
Balls into non-uniform bins
2014
Balls-into-bins games for uniform bins are widely used to model randomized load balancing strategies. Recently, balls-into-bins games have been analysed under the assumption that the selection probabilities for bins are not uniformly distributed. These new models are motivated by properties of many peer-to-peer (P2P) networks, which are not able to perfectly balance the load over the bins. While previous evaluations try to find strategies for uniform bins under non-uniform bin selection probabilities, this paper investigates heterogeneous bins, where the "capacities" of the bins might differ significantly. We show that heterogeneous environments can even help to distribute the load more eve…
Deciding reachability for planar multi-polynomial systems
1996
In this paper we investigate the decidability of the reachability problem for planar non-linear hybrid systems. A planar hybrid system has the property that its state space corresponds to the standard Euclidean plane, which is partitioned into a finite number of (polyhedral) regions. To each of these regions is assigned some vector field which governs the dynamical behaviour of the system within this region. We prove the decidability of point to point and region to region reachability problems for planar hybrid systems for the case when trajectories within the regions can be described by polynomials of arbitrary degree.
Cationic Supramolecular Vesicular Aggregates for Pulmonary Tissue Selective Delivery in Anticancer Therapy
2016
The biopharmaceutical properties of supramolecular vesicular aggregates (SVAs) were characterized with regard to their physicochemical features and compared with cationic liposomes (CLs). Neutral and cationic SVAs were synthesized using two different copolymers of poly(aspartyl hydrazide) by thin-layer evaporation and extrusion techniques. Both copolymers were self-assembled in pre-formulated liposomes and formed neutral and cationic SVAs. Gemcitabine hydrochloride (GEM) was used as an anticancer drug and loaded by a pH gradient remote loading procedure, which significantly increased drug loading inside the SVAs. The resulting average size of the SVAs was 100 nm. The anticancer activity of …
SF3B1 modulators affect key genes in metastasis and drug influx: a new approach to fight pancreatic cancer chemoresistance.
2021
Aim: Because mutations of splicing factor 3B subunit-1 (SF3B1) have been identified in 4% of pancreatic ductal adenocarcinoma (PDAC) patients, we investigated the activity of new potential inhibitors of SF3B1 in combination with gemcitabine, one of the standard drugs, in PDAC cell lines. Methods: One imidazo[2,1-b][1,3,4]thiadiazole derivative (IS1) and three indole derivatives (IS2, IS3 and IS4), selected by virtual screening from an in-house library, were evaluated by the sulforhodamine-B and wound healing assay for their cytotoxic and antimigratory activity in the PDAC cells SUIT-2, Hs766t and Panc05.04, the latter harbouring the SF3B1 mutations. The effects on the splicing pattern of pr…