Search results for "ER STRESS"

Growth and biomass partitioning of Arbutus unedo, Laurus nobilis, Phillyrea angustifolia and Rhamnus alaternus seedlings under water deficit

2013

Water scarcity in the Mediterranean areas is very common and the selection of drought tolerant species is a priority for landscaping purposes in the Mediterranean areas. The objective of this study was to evaluate the effect of water deficit on growth and biomass partitioning of Arbutus unedo, Laurus nobilis, Phillyrea angustifolia and Rhamnus alaternus. Seventy-two seedlings per species were grown in the greenhouse in 4-liter pots and three water regimes were imposed starting on 6 April and until 9 June, 2010. One-third of the plants was well irrigated to maintain soil water content in the 25-35% range (CTR); a second group of plants was deficit-irrigated to maintain soil water content in …

The cytoprotective protein MANF promotes neuronal survival independently from its role as a GRP78 cofactor

2021

Mesencephalic astrocyte-derived neurotrophic factor (MANF) is an endoplasmic reticulum (ER)-stress-regulated protein exhibiting cytoprotective properties through a poorly understood mechanism in various in vitro and in vivo models of neuronal and non-neuronal damage. Although initially characterized as a secreted neurotrophic factor for midbrain dopamine neurons, MANF has recently gained more interest for its intracellular role in regulating the ER homeostasis, including serving as a cofactor of the chaperone glucose-regulated protein 78 (GRP78). We aimed for a better understanding of the neuroprotective mechanisms of MANF. Here we show for the first time that MANF promotes the survival of …

Effect of water stress on dry matter accumulation and partitioning in pot-grown olive trees (cv Leccino and Racioppella)

2013

Abstract Three different water regimes were applied on young pot-grown olive trees of the cultivars Leccino and Racioppella, amounting to 100% (treatment T100), 50% (treatment T50) and 25% (treatment T25) of water transpiration as determined by pot weight. During the two-year trial the following parameters were measured: midday stem water potential, shoots growth, total leaf area per tree, dry matter accumulation and partitioning in different parts of the plant (root, wood, leaves and fruits). Dry matter was affected by the water regime and cultivar. The cv Leccino, for T100, displayed a greater accumulation of total dry matter and fruit dry matter, while these two parameters were greatly r…

Novel combination of celecoxib and proteasome inhibitor MG132 provides synergistic antiproliferative and proapoptotic effects in human liver tumor ce…

2010

Molecular targeted therapy has shown promise as a treatment for advanced hepatocellular carcinoma (HCC). Celecoxib (Celebrex®) exhibits antitumor effects in human HCC cells, and its mechanism of action is mediated either by its ability to inhibit cyclooxygenase 2 (COX-2) or by a number of various other COX-2 independent effects. Proteasome inhibitors (PIs) can exert cell growth inhibitory and apoptotic effects in different tumor cell types, including HCC cells. The present study examined the interaction between celecoxib and the PI MG132 in two human liver tumor cell lines HepG2 and HA22T/VGH. Our data showed that each inhibitor reduced proliferation and induced apoptosis in a dose-dependen…

Regional Soil Moisture Retrieval Using Remotely Sensed Crop Water Stress Indicators

2011

Relationship Between Oxidative Stress, ER Stress, and Inflammation in Type 2 Diabetes: The Battle Continues

2019

Type 2 diabetes (T2D) is a metabolic disorder characterized by hyperglycemia and insulin resistance in which oxidative stress is thought to be a primary cause. Considering that mitochondria are the main source of ROS, we have set out to provide a general overview on how oxidative stress is generated and related to T2D. Enhanced generation of reactive oxygen species (ROS) and oxidative stress occurs in mitochondria as a consequence of an overload of glucose and oxidative phosphorylation. Endoplasmic reticulum (ER) stress plays an important role in oxidative stress, as it is also a source of ROS. The tight interconnection between both organelles through mitochondrial-associated membranes (MAM…

Physiological and Structural Responses to Prolonged Water Deficit in Young Trees of Two Olive Cultivars

2022

This study aimed to characterize the physiological and structural responses of potted one-year-old olive trees belonging to two olive cultivars—‘Nocellara del Belice’ and ‘Cerasuola’—exposed to prolonged drought under greenhouse conditions. Two irrigation treatments based on evapotranspiration (ET) were imposed for 69 days, i.e., well-watered (WW, 100% ET) and drought-stressed (DS, 10–30% ET). Leaf stomatal conductance (gs), stem water potential (Ψstem), transpiration (E), photosynthetic capacity (Amax), water use efficiency (WUE), stem (Kstem) and root (Kroot) hydraulic conductance, trunk diameter variations (TDV), and leaf patch attenuated pre…

Protein Biomarkers of Bovine Defective Meats at a Glance: Gel-Free Hybrid Quadrupole-Orbitrap Analysis for Rapid Screening

2021

An understanding of biological mechanisms that could be involved in the stress response of animal cattle prior to slaughter is critical to create effective strategies aiming at the production of high-quality meat. The sarcoplasmic proteome of directly extracted samples from normal and high ultimate pH (pHu) meat groups was studied through a straightforward gel-free strategy supported by liquid chromatography hybrid quadrupole-Orbitrap high-resolution mass spectrometry (LC-HRMS) analysis. A stepped proteomic pipeline combining rapid biomarker hunting supported by qualitative protein Mascot scores followed by targeted label-free peptide quantification revealed 26 descriptors that characterize…

Olive yield as a function of soil moisture dynamics

2011

This study introduces a water-driven crop model aiming to quantitatively link olive yield to climate and soil moisture dynamics using an ecohydrological approach. A mathematical model describing soil moisture, evapotranspiration and assimilation dynamics of olive orchards is developed here. The model is able to explicitly reproduce two different hydroclimatic phases in Mediterranean areas: the well-watered conditions in which evapotranspiration and assimilation assume their maximum values and the real conditions where the limitations induced by soil moisture availability are taken into account. Annual olive yield is obtained by integrating the carbon assimilation during the growing season, …

Detection of Water Stress in an Olive Orchard with Thermal Remote Sensing Imagery

2006

An investigation of the detection of water stress in non-homogeneous crop canopies such as orchards using high-spatial resolution remote sensing thermal imagery is presented. An airborne campaign was conducted with the Airborne Hyperspectral Scanner (AHS) acquiring imagery in 38 spectral bands in the 0.43–12.5 mm spectral range at 2.5 m spatial resolution. The AHS sensor was flown at 7:30, 9:30 and 12:30 GMT in 25 July 2004 over an olive orchard with three different water-deficit irrigation treatments to study the spatial and diurnal variability of temperature as a function of water stress. A total of 10 AHS bands located within the thermal-infrared region were assessed for the retrieval of…