Search results for "ERAS"
showing 10 items of 4431 documents
Acetylcholinesterase activity in juvenileCiona intestinalis(Ascidiacea, Urochordata) after exposure to tributyltin
2012
The acetylcholinesterase (AChE) activity has been studied in 4-day post-fertilization juveniles of Ciona intestinalis exposed to tributyltin (TBT) at 10-5 M using the histochemical method of Karnovsky and Roots. Relative to vertebrate tissues and organs, the development of adult form of ascidians is interesting, because the analysis of many nuclear genes indicated that the ascidians are the closest living relatives of the vertebrates. Therefore, toxicity research using different approaches could provide data for comparative studies with vertebrates. AChE is over-expressed under chemical stress and in some diseases of vertebrates. Therefore, AChE is considered a biomarker of environmental co…
Casein kinase 2 inhibits HomolD-directed transcription by Rrn7 in Schizosaccharomyces pombe.
2014
In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA analogue element called the HomolD box. The HomolD-binding protein Rrn7 forms a complex with the RNA polymerase II machinery. Despite the importance of ribosome biogenesis to cell survival, the mechanisms involved in the regulation of transcription of eukaryotic RPGs are unknown. In this study, we identified Rrn7 as a new substrate of the pleiotropic casein kinase 2 (CK2), which is a regulator of basal transcription. Recombinant Rrn7 from S. pombe, which is often used as a model organism for studying eukaryotic transcription, interacted with CK2 in vitro and in vivo. Furthermore, CK2-mediated phosphorylation…
Retinoid dynamics in chicken eye during pre- and postnatal development.
1994
Changes in the steady state level of retinols, retinaldehydes and retinyl esters in the trans and 11-cis forms and trans retinoic acid were measured in whole chicken eye during development from day 6 in ovo to day 3 post-hatch. These retinoids, quantified by different HPLC systems, were detected in this time sequence: trans-retinol and trans-retinyl esters in the first week in ovo, 11-cis-retinol in the second week. The highest level of 11-cis-retinaldehyde and 11-cis-retinyl esters was reached at the end of development in ovo; however, their levels increased further after hatching. The retinoic acid level decreased at the end of the first week, rising again at the end of the second week. T…
Chemical composition, antioxidant and anticholinesterase activity of the essential oil of algerian cachrys sicula L
2021
In this work, in order to explore a new Algerian medicinal plant used in traditional medicine, the essential oil of the leaves of Cachrys sicula L. (Apiaceae) collected from Algeria, obtained by hydrodistillation, was analyzed by GC/MS. Thirty-two compounds were identified accounting for 98.6% of the total oil, which is characterized by a high content of monoterpene hydrocarbons (74.8%). The main constituents of the essential oil were β-pinene (17.9%), sabinene (17.8%), myrcene (12%), and α-pinene (11.4%). In vitro antioxidant activity of the essential oil was assayed by three methods, namely ABTS•+, metal chelating, and DPPH• assays. The antioxidant activity of the oil was higher in the AB…
La producción radiofónica en clase de ELE, un recurso motivador para el trabajo de destrezas comunicativas e interculturales
2019
Título, resumen y palabras clave en español e inglés Resumen basado en el de la publicación El estudio está basado en una experiencia en el aula con alumnos adultos de ELE en India. El objetivo es analizar el desarrollo de competencias lingüísticas e interculturales a partir de la producción radiofónica en un entorno social y académico caracterizado por la interacción oral multilingüe y por un aprendizaje en el aula que descansa sobre los pilares de la creatividad y la reflexión. A lo largo de la investigación, se observa cómo el desarrollo de la competencia comunicativa, inherente a la producción radiofónica, integra tanto el componente lingüístico-semántico como el pragmático intercultura…
La tipografía en el cine: : análisis de cabeceras y créditos de películas como estrategia educativa desde la cultura visual
2013
En el presente artículo se recogen un conjunto de relexiones sobre papel que adquieren los textos escritos utilizados como imágenes en el discurso cinematográico. Las letras del alfabeto han estado íntimamente unidas a la tradición del cine desde sus inicios. Esta atracción mutua que ha existido siempre entre el alfabeto y el cine ha generado un extraordinario legado que podemos analizar mediante los postulados de la cultura visual. Sugerimos la oportunidad de utilizar este elemento visual para solventar algunos errores, empobrecimientos, insuiciencias y déicits que pueden observarse en buena parte de los rituales educativos convencionales, ya que las letras como signos son artefactos visua…
The genotypes and virulence attributes of C. albicans isolates from oral leukoplakia
2021
Background There is a debate as to whether some types of oral leucoplakias (OL) are caused by Candida species, and whether they contribute to the malignant transformation, associated with a minority of such lesions. As no detailed population analysis of yeast isolates from OL is available, we evaluated the virulence attributes, and genotypes of 35 C. albicans from OL, and compared their genotypes with 18 oral isolates from healthy individuals. Material and Methods The virulence traits evaluated were esterase, phospholipase, proteinase, haemolysin and coagulase production, and phenotypic switching activity, and yeast adherence and biofilm formation. DNA from OL and control yeasts were evalua…
5-Azacytidine Inhibits the Activation of Senescence Program and Promotes Cytotoxic Autophagy during Trdmt1-Mediated Oxidative Stress Response in Insu…
2022
5-Azacytidine (5-azaC), a methyltransferase inhibitor and anticancer drug, can promote several cellular stress responses such as apoptosis, autophagy, and senescence. The action of 5-azaC is complex and can be modulated by dose, time of treatment, and co-administration with oxidants. Insulinoma is a rare pancreatic neuroendocrine tumor with limited chemotherapeutic options. In the present study, two cellular models of insulinoma were considered, namely NIT-1 and β-TC-6 mouse cells, to evaluate the effects of 5-azaC post-treatment during hydrogen peroxide-induced oxidative stress. 5-azaC attenuated the development of oxidant-induced senescent phenotype in both cell lines. No pro-apoptotic ac…
Expression and purification of polyhistidine-tagged firefly luciferase in insect cells
2001
The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and purified by immobilized metal-ion affinity chromatography in combination with an expanded bed adsorption system. This approach enabled an efficient, one-step purification protocol of a genetically modified luciferase with properties similar to those of the authentic counterpart. According to light emission measurements, the final yield of highly purified protein was 23 mg l−1 of cell culture. In addition, no specific interaction of interfering …
Zaļais publiskais pārtikas iepirkums Latvijas skolās: gadījumu izpēte
2015
Zaļais publiskais iepirkums kļūst par arvien būtiskāku vides pārvaldības instrumentu pasaulē un arī Latvijā. Maģistra darbā tiek pētīts zaļais publiskais iepirkuma pārtikas sektorā Latvijā, īpaši apskatot vides kritēriju izmantošanu skolu pārtikas iepirkumos. Darbā novērtēts esošais zaļā iepirkuma tiesiskais regulējums Eiropas Savienībā un Latvijā, apskatīta zinātniskā literatūra un līdz šim veiktie pētījumi attiecībā uz galvenajām ietekmēm pārtikas produktu dzīves ciklā, to saistība ar vides kritēriju piemērošanu pārtikas iepirkumā. Empīriskajā daļā veikta četru Latvijas skolu, kuras 2015. gada I ceturksnī, veicot pārtikas produktu iepirkumu, izmantojošas saimnieciski izdevīgāko piedāvājum…