Search results for "ERAS"

showing 10 items of 4431 documents

RNA uridylation and decay in plants

2018

RNA uridylation consists of the untemplated addition of uridines at the 3′ extremity of an RNA molecule. RNA uridylation is catalysed by terminal uridylyltransferases (TUTases), which form a subgroup of the terminal nucleotidyltransferase family, to which poly(A) polymerases also belong. The key role of RNA uridylation is to regulate RNA degradation in a variety of eukaryotes, including fission yeast, plants and animals. In plants, RNA uridylation has been mostly studied in two model species, the green algae Chlamydomonas reinhardtii and the flowering plant Arabidopsis thaliana . Plant TUTases target a variety of RNA substrates, differing in size and function. These RNA substrates include …

0106 biological sciences0301 basic medicineSmall interfering RNATerminal nucleotidyltransferaseRNA StabilitymRNAArabidopsisChlamydomonas reinhardtiiUridylationBiology01 natural sciencesRNA decayGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesRNA degradationSettore AGR/07 - Genetica AgrariamicroRNAGene silencing[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyUridineComputingMilieux_MISCELLANEOUSPolymerase2. Zero hungerMessenger RNABiochemistry Genetics and Molecular Biology (all)fungiRNAfood and beverages[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biologyArticlesPlantsRibosomal RNAbiology.organism_classificationCell biology030104 developmental biologyAgricultural and Biological Sciences (all)biology.proteinRNARNA InterferenceGeneral Agricultural and Biological SciencesChlamydomonas reinhardtii010606 plant biology & botany
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Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

2021

The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, named Žilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight % of the samples were positive for at least one virus, and all five viruses were detected, thought with different incidence, i.e. GLRaV-3 (84%), GFLV (43%), GLRaV-1 (14%), GFk…

0106 biological sciences0301 basic medicineVeterinary medicineArtificial Gene Amplification and ExtensionDisease VectorsPathology and Laboratory MedicinePolymerase Chain Reaction01 natural sciencesPlant VirusesMedical ConditionsXiphinemaMedicine and Health SciencesVitisCultivarNematode InfectionsFlowering PlantsPhylogenyData Managementeducation.field_of_studyMultidisciplinarybiologyPhylogenetic treeQREukaryotaPhylogenetic AnalysisGrapevine fanleaf virusPlantsPhylogeneticsInfectious DiseasesMedical MicrobiologyViral PathogensVirusesMedicineRNA ViralGrapevineViral VectorsPathogensPlant ViruseResearch ArticleComputer and Information SciencesSciencePopulationPlant DiseaseResearch and Analysis Methodsno key wordsMicrobiologyVirusArabis mosaic virus03 medical and health sciencesVirologyParasitic DiseasesEvolutionary SystematicsMolecular Biology TechniqueseducationMolecular BiologyMicrobial PathogensTaxonomyPlant DiseasesBosnia and HerzegovinaEvolutionary BiologyGenetic diversityOrganismsBiology and Life SciencesSettore AGR/12 - Patologia VegetaleReverse Transcriptase-Polymerase Chain Reactionbiology.organism_classificationVector-Borne DiseasesSpecies Interactions030104 developmental biologyViral Transmission and Infection010606 plant biology & botany
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Begomoviruses Infecting Tomato Crops in Panama

2015

The key regions in Panama involved in open field- and greenhouse-grown commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santos, Coclé and Panama Oeste provinces, were surveyed for the incidence and distribution of begomoviruses in the growing seasons of 2011 and 2012. The surveys took place in 14 of the 51 districts of the above-mentioned provinces and comprised all relevant tomato production areas of the provinces. A total of 28 tomato plots were surveyed. The exact location of each plot was geo-referenced using a hand-held Global Positioning System unit. In total, 319 individual tomato plants (181 in 2011 and 138 in 2012) were sampled. Plants displayed diverse …

0106 biological sciences0301 basic medicineVeterinary medicinePhysiologyGrowing seasonTomato leaf curl Sinaloa viruPlant ScienceBiology01 natural scienceslaw.invention03 medical and health sciencesSolanum lycopersicumlawGenusBotanyTomato yellow mottle viruGeneticsLeaf sizePolymerase chain reactionPanamafungiBegomovirusSettore AGR/12 - Patologia Vegetalefood and beveragesbiology.organism_classificationPolymerase chain reactionTomato yellow mottle virus030104 developmental biologyKey (lock)Potato yellow mosaic Panama viruAgronomy and Crop Science010606 plant biology & botanyJournal of Phytopathology
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Plant Responses to Abiotic Stress Regulated by Histone Deacetylases

2017

In eukaryotic cells, histone acetylation and deacetylation play an important role in the regulation of gene expression. Histone acetylation levels are modulated by histone acetyltransferases (HATs) and histone deacetylases (HDACs). Recent studies indicate that HDACs play essential roles in the regulation of gene expression in plant response to environmental stress. In this review, we discussed the recent advance regarding the plant HDACs and their functions in the regulation of abiotic stress responses. The role of HDACs in autophagy was also discussed.

0106 biological sciences0301 basic medicineautophagyabiotic stressHistone acetylation and deacetylationMini ReviewPlant Sciencelcsh:Plant culture01 natural sciencesEnvironmental stress03 medical and health scienceschemistry.chemical_compoundhistone deacetylationlcsh:SB1-1110Histone AcetyltransferasesRegulation of gene expressionprotein complexesbiologyAbiotic stressAutophagyHDACsCell biology030104 developmental biologyHistonechemistryAcetylationbiology.protein010606 plant biology & botanyFrontiers in Plant Science
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The In Vitro Interaction of 12-Oxophytodienoic Acid and Related Conjugated Carbonyl Compounds with Thiol Antioxidants

2021

α,β-unsaturated carbonyls interfere with numerous plant physiological processes. One mechanism of action is their reactivity toward thiols of metabolites like cysteine and glutathione (GSH). This work aimed at better understanding these interactions. Both 12-oxophytodienoic acid (12-OPDA) and abscisic acid (ABA) conjugated with cysteine. It was found that the reactivity of α,β-unsaturated carbonyls with GSH followed the sequence trans-2-hexenal &lt

0106 biological sciences0301 basic medicinecysteine covalent modification570Isomerase activityArabidopsis thalianaArabidopsislcsh:QR1-50201 natural sciencesBiochemistryArticleAntioxidantslcsh:Microbiology03 medical and health scienceschemistry.chemical_compoundThioredoxinsPlant Growth RegulatorsmedicineCysteineSulfhydryl CompoundsMolecular BiologyCyclophilinchemistry.chemical_classificationChemistry<i>Arabidopsis thaliana</i>peroxiredoxinGlutathionethioredoxinphytohormones030104 developmental biologyMechanism of actionBiochemistryprotein–ligand interactioncyclophilinThiolFatty Acids Unsaturatedmedicine.symptomThioredoxinPeroxiredoxinthiol antioxidants010606 plant biology & botanyCysteineBiomolecules
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Boric acid toxicity to the German cockroach, Blattella germanica: Alterations in midgut structure, and acetylcholinesterase and glutathione S-transfe…

2006

0048-3575 doi: DOI: 10.1016/j.pestbp.2005.05.002; Oral toxicity of boric acid, an inorganic insecticide, was evaluated on German cockroach, Blattella germanica L. (Dictyoptera, Blattellidae). Newly emerged adults were exposed to various concentrations of boric acid incorporated into the diet. Results showed that treated insects exhibited toxic symptoms with a dose-dependent mortality. Histological study of midgut revealed alterations in the epithelial cells and a signiWcant increase in the epithelium thickness. In a second series of experiments, the compound was investigated on the activities of acetylcholinesterase (AChE) and glutathione S-transferases (GSTs). Data showed that the compound…

0106 biological sciencesAchéHealth Toxicology and MutagenesisBiology01 natural sciences03 medical and health scienceschemistry.chemical_compoundMidgutPesticides030304 developmental biology2. Zero hunger0303 health sciencesGerman cockroachDictyopteraMidgutGeneral MedicineGlutathionebiology.organism_classificationBoric acidAcetylcholinesteraselanguage.human_language3. Good healthGlutathione S-transferase010602 entomologyGlutathione S-transferaseBlattella germanicachemistryBiochemistryToxicitylanguagebiology.proteinAcetylcholinesteraseAgronomy and Crop Science
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Exploration of the Oxynoticeratidae ornamental morphospace using the discrete cosine transform (DCT) to analyze rib patterns.

2006

The discrete cosine transform (DCT) is a Fourier-related transform widely used in signal processing and well suited to analyzing open outlines such as ammonite ribs. The method is applied here to depict and decipher the ribbing morphospace of a large group of Lower Jurassic ammonites composed of the Oxynoticeratidae and their close ancestors. Because they are clearly associated with buoyancy and/or swimming ability, the usually clearly involute, comparatively smooth and compressed shells of these ammonites may well be misleading taxonomic markers. In this context, quantitative analysis of the ribbing pattern using the DCT may significantly improve our perception of the ornamental patterns e…

0106 biological sciencesAmmonite010506 paleontologyOxynoticerasEcologybiologyFeature (archaeology)Range (biology)PaleontologyContext (language use)biology.organism_classification010603 evolutionary biology01 natural scienceslanguage.human_languagePaleontologyGenusGleviceraslanguageDiscrete cosine transform[SDU.STU.PG] Sciences of the Universe [physics]/Earth Sciences/PaleontologyGeneral Agricultural and Biological SciencesEcology Evolution Behavior and Systematics0105 earth and related environmental sciences[ SDU.STU.PG ] Sciences of the Universe [physics]/Earth Sciences/Paleontology
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Responses of a native plant species from invaded and uninvaded areas to allelopathic effects of an invader

2019

Invaders exert new selection pressures on the resident species, for example, through competition for resources or by using novel weapons. It has been shown that novel weapons aid invasion but it is unclear whether native species co‐occurring with in‐ vaders have adapted to tolerate these novel weapons. Those resident species which are able to adapt to new selective agents can co‐occur with an invader while others face a risk of local extinction. We ran a factorial common garden experiment to study whether a native plant species, Anthriscus sylvestris, has been able to evolve a greater tolerance to the allelochemicals exerted by the invader, Lupinus polyphyllus. Lupinus polyphyllus produces …

0106 biological sciencesAnthriscus sylvestrisLupinus polyphyllusmedia_common.quotation_subjectLupinus polyphyllusIntroduced speciesBiology010603 evolutionary biology01 natural sciencesCompetition (biology)03 medical and health scienceslcsh:QH540-549.5Botanyvieraslajitlupiinitevolutionary responseEcology Evolution Behavior and SystematicsAllelopathyOriginal Research030304 developmental biologyNature and Landscape Conservationmedia_commonLocal adaptationsopeutuminen0303 health sciencesEcology15. Life on landNative plantnative plantbiology.organism_classificationinvasionalkuperäiset kasvilajitLocal extinctionlcsh:EcologyAnthriscus sylvestrissarjakukkaiskasvitlocal adaptation
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The antioxidant 2,3‐dichloro,5,8‐dihydroxy,1,4‐naphthoquinone inhibits acetyl‐cholinesterase activity and amyloid β 42 aggregation: A dual target the…

2020

Alzheimer's disease is characterized by amyloid β aggregation and cholinergic neurodegeneration. In the present study, pure DDN (2,3-dichloro-5,8-dihydroxy-1,4-naphthoquinone) was examined, for the first time, for its dual potential as inhibitor of acetylcholinesterase (AChE) and Aβ42 aggregation. Such investigation was encouraged by the in vitro high antioxidant potential of DDN. Indeed, it revealed interesting antioxidant activity with IC50 values of 9.8 and 4.3 µM for ABTS and reducing power, respectively. The ability of DDN to counteract Aβ42 aggregation was evaluated by thioflavine-T assay. Strong inhibition of Aβ42 aggregation of more than 90% at 25 µM was measured. Moreover, results …

0106 biological sciencesAntioxidantAchémedicine.medical_treatmentBiomedical EngineeringBioengineering14-Naphthoquinone01 natural sciencesApplied Microbiology and Biotechnology03 medical and health scienceschemistry.chemical_compound010608 biotechnologyDrug DiscoverymedicineIC50030304 developmental biology0303 health sciencesABTSChemistryProcess Chemistry and TechnologyNeurodegenerationGeneral Medicinemedicine.diseaseAcetylcholinesteraselanguage.human_languageIn vitroBiochemistrylanguageMolecular MedicineBiotechnologyBiotechnology and Applied Biochemistry
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