Search results for "ERT"

The Extracellular δ-Domain is Essential for the Formation of CD81 Tetraspanin Webs

2014

AbstractCD81 is a ubiquitously expressed member of the tetraspanin family. It forms large molecular platforms, so-called tetraspanin webs that play physiological roles in a variety of cellular functions and are involved in viral and parasite infections. We have investigated which part of the CD81 molecule is required for the formation of domains in the cell membranes of T-cells and hepatocytes. Surprisingly, we find that large CD81 platforms assemble via the short extracellular δ-domain, independent from a strong primary partner binding and from weak interactions mediated by palmitoylation. The δ-domain is also essential for the platforms to function during viral entry. We propose that, ins…

SARS-CoV-2 envelope protein topology in eukaryotic membranes

2020

Coronavirus E protein is a small membrane protein found in the virus envelope. Different coronavirus E proteins share striking biochemical and functional similarities, but sequence conservation is limited. In this report, we studied the E protein topology from the new SARS-CoV-2 virus both in microsomal membranes and in mammalian cells. Experimental data reveal that E protein is a single-spanning membrane protein with the N-terminus being translocated across the membrane, while the C-terminus is exposed to the cytoplasmic side (Nt lum /Ct cyt ). The defined membrane protein topology of SARS-CoV-2 E protein may provide a useful framework to understand its interaction with other viral and ho…

Visual Contrast Modulates Operant Learning Responses in Larval Zebrafish.

2018

The larval zebrafish is a promising vertebrate model organism to study neural mechanisms underlying learning and memory due to its small brain and rich behavioral repertoire. Here, we report on a high-throughput operant conditioning system for zebrafish larvae, which can simultaneously train 12 fish to associate a visual conditioned pattern with electroshocks. We find that the learning responses can be enhanced by the visual contrast, not the spatial features of the conditioned patterns, highlighted by several behavioral metrics. By further characterizing the learning curves as well as memory extinction, we demonstrate that the percentage of learners and the memory length increase as the co…

Tiešsaistes apdrošināšanas platformas optimizācija

2018

Vispārīgā datu aizsardzības regula (VDAR) ir paredzēta datu aizsardzībai un privātuma nodrošināšanai visiem pilsoņiem, kas dzīvo Eiropas Savienības valstīs. Viena no šīs regulas prasībām ir datu subjektu pieprasījums (DSP), kas paredz kādas organizācijas pakalpojumu lietotājam pieprasīt par sevi vāktos datus, kas glabājas organizācijā. Arī tiešsaistes apdrošināšanas platformai Bamboo, kas tiek izstrādāta uzņēmumā, ir jānodrošina tās klientiem iegūt par sevi uzglabātos datus. Pašlaik tas tiek nodrošināts manuāli, ejot cauri visiem dokumentiem un lejupielādējot tos pa vienam. Tādēļ šī darba mērķis ir optimizēt platformu un atvieglot darbu zvanu centra darbiniekiem, kas pārvalda klientu datus …

Kohti elävää kuvaa : visuaalinen ajattelu kertovan elokuvan näyttämöllepanon hahmotus- ja suunnitteluprosessissa

2011

Tutkimuksessa tarkastellaan visuaalista ajattelua sekä sen merkitystä kertovan elokuvan näyttämöllepanon suunnittelu- ja hahmotusprosessissa. Visuaalisella ajattelulla käsitetään mielikuvat, visuaalisen havaitsemisen Rudolf Arnheimin teorian mukaan sekä visuaaliseen kerrontaan liittyvän luovan, produktiivisen ajattelun. Tutkimus on osa kognitiivista elokuvatutkimusta ja pohjaa vahvasti hahmopsykologiaan sekä Rudolf Arnheimin visuaalisen havaitsemisen teoriaan. Tutkimuksen alussa perehdytään hahmoteoriaan, jonka perusajatuksena on, että mieli hahmottaa kokonaisuuksia pikemminkin kuin osia, joista kokonaisuudet rakentuvat. Tutkimuksessa käydään läpi Rudolf Arnheimin havaitsemisen teoriaa sekä…

Building a political image on Instagram: A study of the personal profile of Santiago Abascal (Vox) in 2018

2020

Due to Instagram’s growing popularity in Spain, politicians have also begun to turn to this social network increasingly more. Accordingly, this paper analyses the visual and textual discourse of 259 posts published throughout 2018 on the personal Instagram profile of Santiago Abascal, the leader of the party Vox. Insofar as he is the Spanish politician with the highest number of followers on Instagram, the aim here is to analyse how he uses this social network in order to identify possible strategies that justify his growing number of followers. In the analysis, special attention was paid to aspects that might have contributed to the (self)presentation of Abascal and the promotion of his pa…

Endothelial dysfunction and vitamin d status in essential hypertension, prelimary results

2011

Vitamina D, ipertensione arteriosa e sistema renina-angiotensina

2011

I polimorfismi del gene VDR FokI e BsmI non sembrano essere associati all’ipertensione arteriosa

2014

RAZIONALE. Il nostro obiettivo è stato quello di analizzare la relazione tra livelli plasmatici di VitaminaD (VitD) e valori di pressione arteriosa clinica ed ambulatoria in ipertesi essenziali e di identificare un’eventuale associazione fra l’ipertensione e i polimorfismi del gene VDR, FokI e BsmI. CASISTICA E METODI. Abbiamo arruolato 71 ipertesi essenziali e 72 controlli di età compresa fra 18 e 75 anni. Nei pazienti sono stati valutati: pressione arteriosa clinica, monitoraggio ambulatorio della pressione arteriosa delle 24 ore, parametri antropometrici, indagini bioumorali di routine e livelli plasmatici di VitD con metodica HPLC. Entrambi i gruppi sono stati sottoposti a tipizzazione …

Vizuālā naratīva un intertekstuālo atsauču salīdzinājums Džima Džārmuša filmās "Patersons" un "Izdzīvo tikai mīlētāji"

2018

Bakalaura darbā Vizuālā naratīva un intertekstuālo atsauču salīdzinājums Džima Džārmuša filmās “Patersons” (2016) un “Izdzīvo Tikai Mīlētāji” (2013) pētītas un salīdzinātas intertekstuālās atsauces Džima Džārmuša filmās “Izdzīvo Tikai Mīlētāji” (2013) un “Patersons” (2016), kā arī pētīts kā šīs atsauces paskaidro filmu vēstījumu. Darba mērķis ir atrast un salīdzināt intertekstuālās atsauces Dž. Džārmuša filmās “Patersons” un “Izdzīvo Tikai Mīlētāji”, izprast kā tās skaidro naratīvu, kā arī analizēt un salīdzināt filmu vizuālo naratīvu. Pētījuma problēma skaidro kā intertekstuālās atsauces, kuras atrodamas filmās atklāj, paskaidro naratīva nozīmi. Uzdevumi ir meklēt un atlasīt nepieciešamos …