Search results for "EVs"
showing 10 items of 129 documents
Alma Mater: 2014/ Vasara
2014
Andra Čudare. Studentu taisnības bruņinieks Kirils Solovjovs -- Katrīna Žukova. Zinību lauks – atmiņu laiks -- LU māju noslēpumi atklājās Muzeju naktī -- LU Preses centrs. Zinātne – tas ir forši un radoši -- Sarmīte Rutkovska. Futbols kā tikšanās vieta -- LU Zinātnes un tehnikas vēstures muzejs, LU Preses centrs. Fotostāsts. Studentu mode cauri laikiem -- Laine Dobulāne. Atjauno modernākās ērģeles Latvijā -- LU Preses centrs. Praktiķi dalās pieredzē ar topošajiem kolēģiem -- LU Preses centrs. Reportāža. Psihiskas performances un «Ātrā palīdzība» jauno grupu konkursā «Hadrons» -- LU Bibliotēka. Ciparojot šodienai un nākotnei: LU Bibliotēkas pieredze digitalizācijā -- Kārlis Dārznieks. Jau 85…
Valdemāra Murevska 60. gadu jubilejas pasākums ražošanas apvienībā "Radiotehnika": 14 fotogrāfijas
1971
Valdemārs Murevskis (1911-1989) no 1946.g. strādāja rūpnīcā „Radiotehnika” (pazīstama arī kā RRR, A.S. Popova Rīgas radiorūpnīca) – sākumā par vecāko meistaru virpotavas-frēzēšanas cehā, vēlāk – par vecāko inženieri un par Akustikas sektora priekšnieku rūpnīcas Centrālajā laboratorijā. Atrisinājis daudzus sarežģītus tehnoloģiskus uzdevumus, saņēmis vairākas patentapliecības. V. Murevskim piešķirts Goda radista (1955) un Latvijas PSR Nopelniem bagātā zinātnes un tehnikas darbinieka goda nosaukums (1964).
Il figliol prodigo. Percorsi della musica russa nei labirinti dello stalinismo
2022
The text takes its cue from the first French-language version of St. Prokofiev's opera "The Player" (1929) to trace a biography of the composer after his return to the Soviet Union
G26/24 extracellular microvesicles contain both H1° protein and RNA
2015
Extracellular vesicles (EVs) are released into the extracellular space from both tumor and normal brain cells. By releasing EVs which contain FGF2 and VEGF1-2, astrocytes and neurons, co-cultured with brain capillary endothelial cells, are for example able to induce them to form a blood-brain barrier-like monolayer. On the other hand, membrane microvesicles (MVs) shed from G26/24 oligodendroglioma cells, when added to primary cultures of rat cortical neurons, induce neuronal damage; the damaging effects include a strong reduction of neurite outgrowth, and apoptosis in about 75% of the cells3. The same amount of shed MVs induce apoptosis in about 40% of astrocytes4. These effects are probab…
Melanoma cells release extracellular vesicle which contain H1° linker histone as well as RNA-binding proteins which bind to the H1° mRNA
2015
We previously demonstrated that G26/24 oligodendroglioma cells release EVs that contain proteins, such as FasL and TRAIL, which induce apoptosis in rat cortical neurons [1] and astrocytes [2]. We also reported that cancer cells use EVs for transferring, into the environment [3], proteins such as extracellular matrix remodelling proteases [4], and H1°, a differentiation-specific histone [5]. In particular, by releasing H1°, cells could escape differentiation cues [5]. To verify the role of EVs in releasing specific proteins and mRNAs, in this study we used as a model A375 melanoma cells. METHODS EVs were purified from cell culture media as previously reported [1, 2]. T1 RNase-protection assa…
Cancer cells can affect behaviour of neighbouring cells by transferring molecules through extracellular vesicles
2017
Most cells release into the extracellular space membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells are much more active than normal cells in producing EVs. Because of this property, they are able to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in these latter at least some transformed behavior. We previously showed that EVs produced by G26/24 oligodendroglioma cells can horizontally transfer to their neighbours radioactive proteins [2]. In addition, EVs released by these cells contain pro-apoptotic proteins, such as TRAIL and Fas-Ligand, able to induce apoptosis in…
Melanoma cells release extracellular vesicles which contain RNA-binding proteins able to bind the mRNA encoding histone H1°
2015
Extracellular vesicles (EVs) are produced by most prokaryotic and eukaryotic cells; tumour cells, however, release much higher amounts of EVs, which contain cancer-specific proteins and RNAs. Molecules carried by EVs are captured by surrounding cells, which then undergo profound phenotypic modifications. G26/24 oligodendroglioma cells release, for example, EVs containing FasL and TRAIL, which induce apoptosis in rat cortical neurons and astrocytes in culture. By metabolic labelling of cells, EV-mediated horizontal transfer of radioactive proteins was clearly demonstrated. Among the proteins present in EVs produced by oligodendroglioma cells, extracellular matrix remodelling proteases, and t…
RNA as a carrier of epigenetic information
2017
Both prokaryotic and eukaryotic cells release into the extracellular matrix membrane-bound structures of different sizes, origin and composition, collectively called extracellular vesicles (EVs) [1]. Tumor cells, in particular, use EVs to transfer both nucleic acids and proteins to the surrounding normal cells, thus inducing in them transformed behaviours or killing them. G26/24 oligodendroglioma cells, for example, transfer by EVs pro-apoptotic proteins, such as TRAIL and Fas-Ligand [2], extracellular matrix remodelling proteases (such as ADAMTS) [3], and even the H1.0 histone protein [4]. Another tumour cell line, with a different tissue origin (A375 melanoma cells) releases into the medi…
Extracellular vesicles released from melanoma cells contain H1° mRNA-binding proteins, one of which is (probably) MYEF2.
2015
Release of extracellular vesicles (EVs) is a process conserved from prokaryotes to eucaryotes. Although EVs are produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which contain tumour-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL and are able to inhibit neurite outgrowth, and induce apoptosis in about 75% of rat cortical neurons [1] and 40% of astrocytes [2] in culture. By labelling proteins synthesized in one cell type, we also demonstrated EV-mediated horizontal transfer of proteins among brain cells. Interestingly, G2624 release, via EVs, extr…
An In Vitro Model of Glioma Development
2023
Gliomas are the prevalent forms of brain cancer and derive from glial cells. Among them, astrocytomas are the most frequent. Astrocytes are fundamental for most brain functions, as they contribute to neuronal metabolism and neurotransmission. When they acquire cancer properties, their functions are altered, and, in addition, they start invading the brain parenchyma. Thus, a better knowledge of transformed astrocyte molecular properties is essential. With this aim, we previously developed rat astrocyte clones with increasing cancer properties. In this study, we used proteomic analysis to compare the most transformed clone (A-FC6) with normal primary astrocytes. We found that 154 proteins are…