Search results for "EXPRESSION"

Forced Flowering in Mandarin Trees Under Phytotron Conditions

2019

[EN] Phytotron has been widely used to assess the effect of numerous parameters on the development of many species. However, less information is available on how to achieve fast profuse flowering in young fruit trees with this plant growth chamber. This study aimed to outline the design and performance of a fast clear methodology to force flowering in young mandarin trees (cv. Nova and cv. Clemenules) and to analyze the influence of induction intensity on inflorescence type. The combination of a short water stress period with simulated spring conditions (day 13 h, 22 °C, night 11 h, 12 °C) in the phytotron allowed flowers to be obtained only after 68-72 days from the time the experiment beg…

The reduction of selenium(IV) by boreal Pseudomonas sp. strain T5-6-I – Effects on selenium(IV) uptake in Brassica oleracea

2019

Selenium (Se) is an essential micronutrient but toxic when taken in excessive amounts. Therefore, understanding the metabolic processes related to selenium uptake and bacteria-plant interactions coupled with selenium metabolism are of high importance. We cultivated Brassica oleracea with the previously isolated heterotrophic aerobic Se(IV)-reducing Pseudomonas sp. T5-6-I strain to better understand the phenomena of bacteria-mediated Se(IV) reduction on selenium availability to the plants. B. oleracea grown on Murashige and Skoog medium (MS-salt agar) with and without of Pseudomonas sp. were amended with Se(IV)/75Se(IV), and selenium transfer into plants was studied using autoradiography and…

Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions

2021

13 Pág. Instituto de Ciencias Forestales (ICIFOR)

Insect Cells for Heterologous Production of Recombinant Proteins

2010

Heterologous gene expression has become an indispensable and powerful tool for the production and subsequent functional analysis of proteins that are difficult to purify from their natural sources. Furthermore, it is the method of choice for the production of variants by introducing site-specific mutations into the DNA encoding the protein of interest. However, many systems are biased by disadvantages. The inability of bacteria to confer important post-translational modifications often results in functional failure of the recombinant protein. In addition, disulfide bonds are not formed properly in bacterial systems. Mammalian cells on the other hand modify properly, but they generally provi…

A critical role of plastidial glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase in the control of plant metabolism and development

2009

3 páginas.

Subliminal fear priming potentiates negative facial reactions to food pictures in women with anorexia nervosa.

2010

BackgroundTo investigate hedonic reactivity and the influence of unconscious emotional processes on the low sensitivity to positive reinforcement of food in anorexia nervosa (AN).MethodAN and healthy women were exposed to palatable food pictures just after a subliminal exposure to facial expressions (happy, disgust, fear and neutral faces), either while fasting or after a standardized meal (hungerversussatiety). Both implicit [facial electromyographic (EMG) activity from zygomatic and corrugator muscles, skin conductance, heart rate, and videotaped facial behavior] and explicit (self-reported pleasure and desire) measures of affective processes were recorded.ResultsIn contrast to healthy wo…

Polycomb-like 2 Associates with PRC2 and Regulates Transcriptional Networks during Mouse Embryonic Stem Cell Self-Renewal and Differentiation

2010

SummaryPolycomb group (PcG) proteins are conserved epigenetic transcriptional repressors that control numerous developmental gene expression programs and have recently been implicated in modulating embryonic stem cell (ESC) fate. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC-fate determination. Using multiple biochemical strategies, we provide evidence that PCL2 is a Polycomb Repressive Complex 2 (PRC2)-associated protein in mouse ESCs. Knockdown of Pcl2 in ESCs resulted in heightened self-renewal characteristics, defects in differentiation, and alte…

Colorectal Cancer Stem Cells: From the Crypt to the Clinic

2014

Since their first discovery, investigations of colorectal cancer stem cells (CSCs) have revealed some unexpected properties, including a high degree of heterogeneity and plasticity. By exploiting a combination of genetic, epigenetic, and microenvironmental factors, colorectal CSCs metastasize, resist chemotherapy, and continually adapt to a changing microenvironment, representing a formidable challenge to cancer eradication. Here, we review the current understanding of colorectal CSCs, including their origin, relationship to stem cells of the intestine, phenotypic characterization, and underlying regulatory mechanisms. We also discuss limitations to current preclinical models of colorectal …

Regulatory Network Of Angiogenesis Gene Expression During Post-Pneumonectomy Compensatory Growth

2011

Atdzīvinot "Morgu": vācu ekspresionista Gotfrīda Benna agrīnās dzejas latviskojumu teorija un prakse

2015

Bakalaura darbā pētīti vācu ekspresionista Gotfrīda Benna agrīnās dzejas atdzejojumi latviešu valodā. Darba mērķis ir atklāt, pēc kādiem principiem vadījušies latviešu atdzejotāji, latviskojot Benna dzejoļu krājuma “Morgs un citi dzejoļi” dzejoļus. Darba pirmajā daļā noskaidroti vadošu ārzemju un latviešu tulkošanas un atdzejošanas pētnieku aprakstītie tulkošanas un atdzejošanas principi un to daudzveidība. Otrajā darba daļā iezīmēts Gotfrīda Benna dzīves gājums un dzejas īpašības, raksturota vācu ekspresionisma poētika, kā arī veikta Gotfrīda Benna dzejoļu atdzejojumu analīze un salīdzināšana. Darbā secināts, ka latviešu atdzejas tradīcijā atzīstami tādi atdzejojumi, kuri tiecas atveidot o…