Search results for "Echinostoma"
showing 10 items of 55 documents
Análisis de la interacciones parásito-hospedador en el modelo Echinostoma caproni-roedor, con énfasis en los factores que determinan el curso de la i…
2015
En la presente Tesis Doctoral se analizan diferentes parámetros implicados en las relaciones parásito-hospedador en helmintiasis intestinales mediante la utilización del modelo experimental Echinostoma caproni-roedor. Con esta finalidad se estudian diversos aspectos inmunológicos, además de las alteraciones intestinales inducidas por la infección en dos sistemas parásito-hospedador: E. caproni-ratón y E. caproni-rata. Los resultados obtenidos ponen de manifiesto que el IFN-γ y el óxido nítrico son mediadores fundamentales en el desarrollo de infecciones crónicas en ratón, induciendo disfunción mitocondrial en las células del epitelio intestinal e hiperplasia tisular. No obstante, se sugiere…
Immunology and pathology of echinostome infections in the definitive host.
2008
This chapter examines the salient literature on the immunology and pathology of members of the family Echinostomatidae in their definitive hosts, with emphasis on experimental studies that may provide useful information on factors that determine resistance to the parasites. For this purpose, several topics such as manifestations and mechanisms of resistance to infection, experimental strategies, and antigenic characterization of echinostomes are covered. Moreover, other topics such as immunodiagnosis are also analyzed. The analysis is focused on members of the genus Echinostoma. Although some of the nomenclature for echinostome species is disputed, the names used are those currently accepte…
Immunology and Pathology of Intestinal Trematodes in Their Definitive Hosts
2006
This review examines the significant literature on the immunology and pathology of intestinal trematodes in their definitive hosts. We emphasize information on selected species in six families for which the literature on these topics is extensive. The families are Brachylaimidae, Diplostomidae, Echinostomatidae, Gymnophallidae, Heterophyidae, and Paramphistomidae. For most of these families, coverage is considered under the following headings: (i) background; (ii) pathology of the infection; (iii) immunology of the infection; (iv) immunodiagnosis; and (v) human infection. Some of these heading have been subdivided further, based on the literature available on a particular topic. Following t…
Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
2016
Background Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments. Methods To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was per…
Extracellular vesicles from parasitic helminths contain specific excretory/secretory proteins and are internalized in intestinal host cells.
2012
The study of host-parasite interactions has increased considerably in the last decades, with many studies focusing on the identification of parasite molecules (i.e. surface or excretory/secretory proteins (ESP)) as potential targets for new specific treatments and/or diagnostic tools. In parallel, in the last few years there have been significant advances in the field of extracellular vesicles research. Among these vesicles, exosomes of endocytic origin, with a characteristic size ranging from 30-100 nm, carry several atypical secreted proteins in different organisms, including parasitic protozoa. Here, we present experimental evidence for the existence of exosome-like vesicles in parasitic…
Identification of proteins in excretory/secretory extracts of Echinostoma friedi (Trematoda) from chronic and acute infections.
2006
In the present study, we describe the investigation of Echinostoma friedi excretory/secretory products using a proteomic approach combined with the use of heterologous antibodies. We have identified 18 protein spots corresponding to ten proteins, including cytoskeletal proteins like actin, tropomyosin, and paramyosin; glycolytic enzymes like enolase, glyceraldehyde 3P dehydrogenase, and aldolase; detoxifying enzymes like GSTs; and stress proteins like heat shock protein (Hsp) 70. Among these proteins, both actin and, to a lesser extent, Hsp70, exhibited differential expression patterns between chronic and acute infections in the Echinostoma-rodent model, suggesting that these proteins may p…
Interactions related to non-host snails in the host-finding process of Euparyphium albuferensis and Echinostoma friedi (Trematoda: Echinostomatidae) …
2003
In order to determine whether the miracidia of Euparyphium albuferensis and Echinostoma friedi are sensitive to their host snail (HS) and capable of discriminating between HS and non-host snails (NHS), or whether these NHS can interfere and thus reduce the infection rates (decoy effect), a total of three experiments were conducted with HS, NHS and snail-conditioned water (SCW). Gyraulus chinensis is the HS for E. albuferensis miracidia, while Physa acuta, Radix peregra and Lymnaea fuscus are considered NHS. For E. friedi miracidia R. peregra, G. chinensis and L. fuscus are the HS, while P. acuta is the NHS. The NHS R. peregra produces the greatest decoy effect on Euparyphium albuferensis mi…
Miracidial infectivity of Hypoderaeum conoideum (Trematoda: Echinostomatidae): differential susceptibility of two lymnaeid species.
1999
A study was made of the infectivity of Hypoderaeum conoideum miracidia to a range of laboratory-reared specimens of freshwater snail species (Lymnaea peregra, L. corvus, Physella acuta, and Gyraulus chinensis) that coexist with the parasite in the same natural habitat. L. peregra and L. corvus were found to be equally susceptible to the parasite when specimens of each snail species were singly exposed to miracidia. However, when miracidia could choose either lymnaeid species, they showed a high degree of specificity toward L. peregra. The results obtained suggest that H. conoideum miracidia are capable of distinguishing among these lymnaeids in their orientation to the host. This indicates …
Sympatric and allopatric experimental infections of the planorbid snail Gyraulus chinensis with miracidia of Euparyphium albuferensis (Trematoda: Ech…
2010
AbstractAn experimental infection with echinostomatid miracidia in sympatric or ‘local’ vs. allopatric or ‘away’ snail combinations, as a model to examine parasite compatibility, was carried out. We employedEuparyphium albuferensismiracidia to infectGyraulus chinensissnails, from three different natural parks: Albufera (Valencia, Spain); the Ebro Delta (Tarragona, Spain) and Coto de Doñana (Huelva, Spain). Insignificant differences between the three snail strains were noted for the infection rate and the rhythm of daily cercarial production. However, a significantly higher total cercarial production per snail, patent period and life span were observed in local snails. The different infectio…
Chapter 3 Recent Advances in the Biology of Echinostomes
2009
This chapter examines the significant literature on the biology of echinostomes. The members of the family Echinostomatidae are medically and veterinary-important parasitic flatworms that invade humans, domestic animals and wildlife and also parasitize in their larval stages numerous invertebrate and cold-blooded vertebrate hosts. All echinostomes possess a complicated lifecycle expressed by: (i) alternation of seven generations known as the adult, egg, miracidium, sporocyst, redia, cercaria and metacercaria, and (ii) inclusion of three host categories known as the definitive host and first and second intermediate hosts. Moreover, echinostomes have served as experimental models in parasitol…