Search results for "Electrochemiluminescence"

showing 6 items of 6 documents

Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

2021

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly due to the inhibitor specificity, toxicity, and drug interactions. Here, we reported that three polyoxypregnanes (POPs) as the most abundant constituents of Marsdenia tenacissima (M. tenacissima) were novel ABCB1-modulatory pro-drugs, which underwent intestinal microbiota-mediated biotransformation in vivo to generate active metabolites. The metabolites at non-toxic concentrations restored chemosensitivity in ABCB1-overexpressing cancer cells v…

ABCC1 ATP binding cassette subfamily C member 1IC50 half maximal inhibitory concentrationMultidrug resistancePharmacologyNADPH reduced nicotinamide adenine dinucleotide phosphateF bioavailabilitychemistry.chemical_compoundPCR polymerase chain reaction0302 clinical medicineMDR multidrug resistanceECL electrochemiluminescencet1/2 elimination half-lifeLC–MS liquid chromatography coupled with mass spectrometryN.D. not detectedGeneral Pharmacology Toxicology and PharmaceuticsBBB blood–brain barriermedia_commonATF3 activating transcription factor 30303 health sciencesChemistryABC ATP-binding cassetteNMPA National Medical Products AdministrationPXR pregnane X receptorSDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresisHBSS Hankʹs balanced salt solutionABCB1Combination chemotherapyProdrugMarsdenia tenacissimaCmax peak concentrationPaclitaxelGAPDH glyceraldehyde-3-phosphate dehydrogenase030220 oncology & carcinogenesisBHI brain heart infusionOriginal ArticleAUC0–∞ area under plasma concentration vs. time curveMRT mean residence timeDrugmedia_common.quotation_subjectRM1-950Vd volume of distributionABCB1 ATP binding cassette subfamily B member 1UIC-2 mouse monoclonal ABCB1 antibodyABCG2 ATP binding cassette subfamily G member 2Combination chemotherapyCYP cytochrome P450 isozymePI propidium iodideTEER transepithelial electrical resistance03 medical and health sciencesPBS phosphate buffer salineFBS fetal bovine serumDox doxorubicinIn vivoPOP polyoxypregnanemedicine030304 developmental biologyEVOM epithelial tissue voltohmmeterTmax time for peak concentrationCancerLBE lowest binding energyPE phycoerythrinmedicine.diseaseMultiple drug resistancePolyoxypregnanePapp apparent permeabilityN.A. not applicableCancer cellH&E hematoxylin and eosinMDR1a multidrug resistance protein 1aTherapeutics. PharmacologyqPCR quantitative PCRM. tenacissima Marsdenia tenacissimaCL clearanceSD standard derivationActa Pharmaceutica Sinica B
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Hot electron injection into aqueous electrolyte solution from thin insulating film-coated electrodes

1998

Hot electron injection into aqueous electrolyte solution was studied with electrochemiluminescence and electron paramagnetic resonance (EPR) methods. Both methods provide further indirect support for the previously proposed hot electron emission mechanisms from thin insulating film-coated electrodes to aqueous electrolyte solution. The results do not rule out the possibility of hydrated electron being as a cathodic intermediate in the reduction reactions at cathodically pulse-polarized thin insulating film-coated electrodes. However, no direct evidence for electrochemical generation of hydrated electrons could be obtained with EPR, only spin-trapping experiments could give information about…

ChemistryHealth Toxicology and MutagenesisInorganic chemistryPublic Health Environmental and Occupational HealthAnalytical chemistryElectronSolvated electronElectrochemistryPollutionRedoxAnalytical ChemistryCathodic protectionlaw.inventionNuclear Energy and EngineeringlawElectrodeElectrochemiluminescenceRadiology Nuclear Medicine and imagingElectron paramagnetic resonanceSpectroscopyJournal of Radioanalytical and Nuclear Chemistry
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An aptamer-gated silica mesoporous material for thrombin detection.

2013

An aptamer-capped mesoporous material for the selective and sensitive detection of &-thrombin in human plasma and serum has been prepared and characterised.

INGENIERIA DE LA CONSTRUCCIONSilicon dioxideAptamerInorganic chemistryNanoparticleAptamersCatalysisFluorescencechemistry.chemical_compoundThrombinQUIMICA ORGANICAQUIMICA ANALITICAMaterials ChemistrymedicineElectrochemiluminescenceHumansHuman Alpha-ThrombinElectrochemiluminescenceMagnetic beadssilicon dioxidePropylaminesChemistryRhodaminesQUIMICA INORGANICAMetals and AlloysThrombinGeneral ChemistryAptamers NucleotideSilanesSilicon DioxideFluorescenceSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsSpectrometry FluorescenceHuman plasmaCeramics and CompositesNanoparticlesMesoporous materialNucleotidePorosityColorimetric detectionmedicine.drugNuclear chemistryChemical communications (Cambridge, England)
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Peptide based biosensors

2018

Abstract This review provides an overview of the various types of (bio)sensors based on peptides for their analytical use, along with significant advances over the last several years in related technologies. So, it will be described: i) principles in biosensing using peptides ii) aspects of fabrication in the perspective of (bio)sensing applications iii) potential of electrochemical, electrochemiluminescence, photoelectrochemical, and optical (bio)sensors for the determination of target analytes within sub-nanomolar range also discussing the main problems in (bio)sensing iv) multiplex electrochemical and optical (bio)sensors, both with and without labels. v) Latest developments in the appli…

Materials scienceSensing applications010401 analytical chemistryMicrofluidicsNanotechnology02 engineering and technology021001 nanoscience & nanotechnology01 natural sciences0104 chemical sciencesAnalytical ChemistryTumor BiomarkersElectrochemiluminescenceMultiplex0210 nano-technologyBiosensorSpectroscopyTrAC Trends in Analytical Chemistry
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Wide-Bite-Angle Diphosphine Ligands in Thermally Activated Delayed Fluorescent Copper(I) Complexes: Impact on the Performance of Electroluminescence …

2021

We report a series of seven cationic heteroleptic copper(I) complexes of the form [Cu(P^P)(dmphen)]BF4, where dmphen is 2,9-dimethyl-1,10-phenanthroline and P^P is a diphosphine chelate, in which the effect of the bite angle of the diphosphine ligand on the photophysical properties of the complexes was studied. Several of the complexes exhibit moderately high photoluminescence quantum yields in the solid state, with ΦPL of up to 35%, and in solution, with ΦPL of up to 98%. We were able to correlate the powder photoluminescence quantum yields with the % Vbur of the P^P ligand. The most emissive complexes were used to fabricate both organic light-emitting diodes and light-emitting electrochem…

PhotoluminescenceLigandCationic polymerizationchemistry.chemical_element02 engineering and technologyBite angle010402 general chemistry021001 nanoscience & nanotechnologyElectrochemistry01 natural sciencesCopper0104 chemical sciencesInorganic ChemistryCrystallographychemistryElectrochemiluminescenceChelationPhysical and Theoretical Chemistry0210 nano-technologyInorganic chemistry
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Current advancement in immunosensing of p53 tumor suppressor protein based on nanomaterials: Analytical approach

2017

Abstract The utility of detection of p53 in tomorrow's personalized health care field will mean early and accurate diagnosis of many types of cancers. This review is meant to provide an overview of the various types of immunosensors and immunoassay have been developed for determination of p53, along with significant advances over the last several years in related technologies. In addition, this review described: • Most frequently applied principles in immunoassay/immunosensing of p53. • The aspects of fabrication in the perspective of immunoassay/immunosensing applications. • The potential of various electrochemical, electrochemiluminescence immunosensors for the determination of p53 within…

medicine.diagnostic_testComputer science010401 analytical chemistryNanotechnologyPersonalized health02 engineering and technology021001 nanoscience & nanotechnology01 natural sciences0104 chemical sciencesAnalytical ChemistryTumor BiomarkersImmunoassayP53 Tumor Suppressor ProteinmedicineElectrochemiluminescence0210 nano-technologySpectroscopyTrAC Trends in Analytical Chemistry
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