Search results for "Electroretinogram"

Rev-Erb modulates retinal visual processing and behavioral responses to light

2016

International audience; The circadian clock is thought to adjust retinal sensitivity to ambient light levels, yet the involvement of specific clock genes is poorly understood. We explored the potential role of the nuclear receptor subfamily 1, group D, member 1 (REV-ERB; or NR1D1) in this respect. In light-evoked behavioral tests, compared with wild-type littermates, Rev-Erb(-/-) mice showed enhanced negative masking at low light levels (0.1 lx). Rev-Erb(-/-) mouse retinas displayed significantly higher numbers of intrinsically photosensitive retinal ganglion cells (ipRGCs; 62% more compared with wild-type) and more intense melanopsin immunostaining of individual ipRGCs. In agreement with a…

Tyro3 Contributes to Retinal Ganglion Cell Function, Survival and Dendritic Density in the Mouse Retina

2020

Retinal ganglion cells (RGCs) are the only output neurons of the vertebrate retina, integrating signals from other retinal neurons and transmitting information to the visual centers of the brain. The death of RGCs is a common outcome in many optic neuropathies, such as glaucoma, demyelinating optic neuritis and ischemic optic neuropathy, resulting in visual defects and blindness. There are currently no therapies in clinical use which can prevent RGC death in optic neuropathies; therefore, the identification of new targets for supporting RGC survival is crucial in the development of novel treatments for eye diseases. In this study we identify that the receptor tyrosine kinase, Tyro3, is crit…

Retinal function assessment in alcohol use disorder patients

2019

Objective. Retinal injury induced by ethanol consumption has been previously reported in animal models, including biochemical, histological and functional alterations. These results need to be clinically tested in alcoholic patients which do not report several systemic or ophthalmic diseases. Methods. Six patients with alcohol use disorder were recruited from an ‘Alcoholism Treatment Unit’. All of them with active alcohol consumption when the study was conducted or that had stopped drinking six months prior to the study, with no ocular disease or visual acuity alterations. All patients underwent fundus photography, optical coherence tomography (OCT) as well as visual field test. Electrophys…

Decreasing dietary linoleic acid promotes long chain omega-3 fatty acid incorporation into rat retina and modifies gene expression

2011

International audience; Age-related macular degeneration (AMD) may be partially prevented by dietary habits privileging the consumption of ω3 long chain polyunsaturated fatty acids (ω3s) while lowering linoleic acid (LA) intake. The present study aimed to document whether following these epidemiological guidelines would enrich the neurosensory retina and RPE with ω3s and modulate gene expression in the neurosensory retina. Rat progenitors and pups were fed with diets containing low or high LA, and low or high ω3s. After scotopic single flash and 8-Hz-Flicker electroretinography, rat pups were euthanized at adulthood. The fatty acid profile of the neurosensory retina, RPE, liver, adipose tis…

Active learning in a real-world bioengineering problem: A pilot-study on ophthalmologic data processing

2019

Active learning is a format alternative to the conventional lecture/recitation/laboratory; research results have reported that it is suitable to encourage student inquiry and foster peer mentoring. Although the availability of computer-based learning materials in biomedical sciences is increasing, there are relatively few studies aimed to integrate traditional methods of teaching with inquiry-based approaches utilizing these Information and Communication Technologies (ICT) tools. This paper describes a pilot-study on a comprehensive active laboratory course about digital ophthalmologic signal classification, experienced by a group of undergraduates in Bio-Electronic Engineering. During the …

An approach based on wavelet analysis for feature extraction in the electroretinogram

2011

Most biomedical signals are non-stationary. The knowledge of their frequency content and temporal distribution is then useful in a clinical context. The wavelet analysis is appropriate to achieve this task. The present paper uses this method to reveal hidden characteristics and anomalies of the human a-wave, an important component of the electroretinogram since it is a measure of the functional integrity of the photoreceptors. We here analyse the time–frequency features of the a-wave both in normal subjects and in patients affected by Achromatopsia, a pathology disturbing the functionality of the cones. The results indicate the presence of two or three stable frequencies that, in the pathol…

Time-frequency analysis of the human photoreceptoral response

2009

Empirical mode decomposition and neural network for the classification of electroretinographic data

2013

The processing of biosignals is increasingly being utilized in ambulatory situations in order to extract significant signals' features that can help in clinical diagnosis. However, this task is hampered by the fact that biomedical signals exhibit a complex behaviour characterized by strong non-linear and non-stationary properties that cannot always be perceived by simple visual examination. New processing methods need be considered. In this context, we propose to apply a signal processing method, based on empirical mode decomposition and artificial neural networks, to analyse electroretinograms, i.e. the retinal response to a light flash, with the aim to detect and classify retinal diseases…

Transient reduction of the ocular perfusion pressure and the oscillatory potentials of the ERG

2005

Abstract Purpose. To evaluate the changes of the Oscillatory Potentials (OPs) of Electroretinogram (ERG) caused by short-term hypertension in human subjects, and their relationship with ocular perfusion pressure (OPP). Methods. Suction cup technique in 12 normal volunteers with OPs simultaneously recording. Results. Scotopic and photopic OPs were altered during OPP drop. Scotopic OPs showed more sensitiveness, with higher reduction (from 21% to 47%), when compared to the basal value, than in photopic recordings (from 14% to 34%). In both conditions, the relationship between OPP and OPs presented a steady amplitude before the trough after the +30 step, and rapid recovery after OPP normalisat…

A study of the human rod and cone electroretinogram a-wave component

2009

The study of the electrical response of the retina to a luminous stimulus is one of the main fields of research in ocular electrophysiology. The features of the first component (a-wave) of the retinal response reflect the functional integrity of the two populations of photoreceptors: rods and cones. We fit the a-wave for pathological subjects with functions that account for possible mechanisms governing the kinetics of the photoreceptors. The paper extends a previous analysis, carried out for normal subjects, in which both populations are active, to patients affected by two particular diseases that reduce the working populations to only one. The pathologies investigated are Achromatopsia, a…