Search results for "Embryo"
showing 10 items of 1872 documents
Gametic embryogenesis through isolated microspore culture in Corylus avellana L.
2015
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report…
Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives
2019
Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advan…
Autotetraploid Emergence via Somatic Embryogenesis in Vitis vinifera Induces Marked Morphological Changes in Shoots, Mature Leaves, and Stomata
2021
Polyploidy plays an important role in plant adaptation to biotic and abiotic stresses. Alterations of the ploidy in grapevine plants regenerated via somatic embryogenesis (SE) may provide a source of genetic variability useful for the improvement of agronomic characteristics of crops. In the grapevine, the SE induction process may cause ploidy changes without alterations in DNA profile. In the present research, tetraploid plants were observed for 9.3% of ‘Frappato’ grapevine somatic embryos regenerated in medium supplemented with the growth regulators β-naphthoxyacetic acid (10 µM) and N6-benzylaminopurine (4.4 µM). Autotetraploid plants regenerated via SE without detectable changes in the …
Maritime Pine Pinus Pinaster Aiton
2018
Maritime pine (Pinus pinaster Aiton) is the most abundant conifer in the Mediterranean basin. Currently, maritime pine is considered to be a model conifer species for study of the adaption responses to drought stress from a genomics approach. In this context, the availability of protocols that allow not only mass vegetative propagation of selected families or genotypes, but also facilitate the functional analyses needed to verify and further to study the effects of candidate genes are necessary. Here we describe an improved protocol to generate maritime pine plants through somatic embryogenesis from immature megagametophytes. Accurate procedures for explant preparation, somatic embryo induc…
Induction of radiata pine somatic embryogenesis at high temperatures provokes a long-term decrease in dna methylation/hydroxymethylation and differen…
2020
Based on the hypothesis that embryo development is a crucial stage for the formation of stable epigenetic marks that could modulate the behaviour of the resulting plants, in this study, radiata pine somatic embryogenesis was induced at high temperatures (23 °
New Approaches to Optimize Somatic Embryogenesis in Maritime Pine
2019
Maritime pine (Pinus pinaster Aiton) is a coniferous native of the Mediterranean basin. Because of its adaptability to a wide range of environmental conditions, the species have become a model for studies in coniferous forest management and functional genomics. Somatic embryogenesis (SE) has been so far, the preferred biotechnological strategy for maritime pine breeding programs initiated at the middle-end of the 20th century. To overcome the limitations of the induction and maturation phases in maritime pine SE, we analyzed the possible maternal influence on the embryogenic capability of megagametophytes from controlled crosses, as well as the effect of the temperature and water availabili…
Maternal effects on offspring Igs and egg size in relation to natural and experimentally improved food supply
2008
1. Maternal effects have been suggested to function as a mechanism for transgenerational plasticity, in which the environment experienced by the mother is translated into the phenotype of the offspring. In birds and other oviparous vertebrates where early development is within the egg, mothers may be able to improve the viability prospects of their offspring at hatching by priming eggs with immunological and nutritional components. 2. We studied how resource availability affects maternal investment in offspring by feeding Ural owl (Strix uralensis, Pall.) females prior to egg-laying in 3 years of dramatically different natural food conditions. 3. Supplementary feeding prior to laying increa…
Direct and transgenerational effects of an experimental heat wave on early life stages in a freshwater snail
2018
AbstractGlobal climate change imposes a serious threat to natural populations of many species. Estimates of the effects of climate change-mediated environmental stresses are, however, often based only on their direct effects on organisms, and neglect the potential transgenerational effects. We investigated whether high temperature (i.e. an experimental heat wave) that is known to reduce performance of adultLymnaea stagnalissnails affects their offspring through maternal effects. Specifically, we tested whether eggs and hatched juveniles are affected by maternal thermal environment, and how strong these effects are compared with direct effects of temperature on offspring. We examined the eff…
Multiple-stressor effects of warming and acidification on the embryonic development of an estuarine fiddler crab
2021
Made available in DSpace on 2021-06-25T10:56:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-06-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Predicted effects of anthropogenic climate change on estuarine and coastal organisms are complex, and early life history stages of calcified ectotherms are amongst the most sensitive groups. Despite the importance of understanding their vulnerability, we lack information on the effects of multiple stressors on the embryonic development of estuarine and burrowing organisms, mainly mangrove-associated species. Here, we determined the combined effects of elevated temperature and decreased pH on the embryonic development of th…
Early exposure to predation risk carries over metamorphosis in two distantly related freshwater insects
2017
1. Predation and competition play a central role in ecological communities, and it is increasingly recognised that animals use early warning cues to reduce the impact of these antagonistic interactions. 2. Strategies to avoid risk can occur during embryo development through plasticity in egg hatching time. This strategy, and its associated costs and carryover effects on adults are little understood in insects. In this study, these are explored in two distantly related freshwater insects: the damselfly Ischnura elegans and the mosquito Aedes albopictus. 3. As predicted, damselfly eggs hatched earlier in response to larval predators cues, a treatment that also affected adult size. Risk cues d…