Search results for "Embryonic Structures"
showing 10 items of 623 documents
Somatic embryogenesis from hypocotyl callus cultures of Digitalis obscura L.
1986
Hypocotyl-derived calli obtained in agar solidified medium with several growth regulator combinations gave rise to proembryonal masses and globular embryos when transferred to liquid media with lower growth regulator and higher NH4HO3 levels. By transferring cultures from liquid media to different solidified media, new embryo formation took place, but further development of these embryos or those previously induced depended on the characteristics of these media. Normal development was only achieved on 8 g/l agar solidified medium without growth regulators. Typical cotyledonary embryos developed into whole plants when transferred to this same medium.
Expression of engrailed in embryos of a beetle and five dipteran species with special reference to the terminal regions
1994
The engrailed expression in embryos of a beetle, four midges and a fly has been analysed with special reference to the terminal regions. In all six species the segmental expression pattern is very similar but variability occurs in the clypeolabrum, foregut and hindgut. In some cases, segmental engrailed expression seems to be extended into the hind- and/or foregut. The engrailed expression of these species is compared with published data from other insects.
Visualization 02.avi
2020
Rotating video of 3D cluster of zebrafish
Visualization 02.avi
2020
Rotating video of 3D cluster of zebrafish
Die endokrinen dr�sen im embryo von Oncopeltus fasciatus Dallas (Insecta, Heteroptera)
1972
The differentiation of the endocrine glands in the embryo of Oncopeltus fasciatus is described. The function of these glands can be correlated with the embryonic moults. The nuclei of some tissues already become polyploid in the embryo. It is discussed whether the endomitotic growth is dependent upon the function of the endocrine glands.
Retinoid dynamics in chicken eye during pre- and postnatal development.
1994
Changes in the steady state level of retinols, retinaldehydes and retinyl esters in the trans and 11-cis forms and trans retinoic acid were measured in whole chicken eye during development from day 6 in ovo to day 3 post-hatch. These retinoids, quantified by different HPLC systems, were detected in this time sequence: trans-retinol and trans-retinyl esters in the first week in ovo, 11-cis-retinol in the second week. The highest level of 11-cis-retinaldehyde and 11-cis-retinyl esters was reached at the end of development in ovo; however, their levels increased further after hatching. The retinoic acid level decreased at the end of the first week, rising again at the end of the second week. T…
Beneficial Effect of Two Culture Systems with Small Groups of Embryos on the Development and Quality of In Vitro-Produced Bovine Embryos
2014
Summary Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro ferti…
Deep learning enables robust assessment and selection of human blastocysts after in vitro fertilization
2019
AbstractVisual morphology assessment is routinely used for evaluating of embryo quality and selecting human blastocysts for transfer after in vitro fertilization (IVF). However, the assessment produces different results between embryologists and as a result, the success rate of IVF remains low. To overcome uncertainties in embryo quality, multiple embryos are often implanted resulting in undesired multiple pregnancies and complications. Unlike in other imaging fields, human embryology and IVF have not yet leveraged artificial intelligence (AI) for unbiased, automated embryo assessment. We postulated that an AI approach trained on thousands of embryos can reliably predict embryo quality with…
Luun morfogeneettisen proteiini 4:n kohdegeenien SKIL ja ID2 vaikutukset rintasyöpäsoluihin
2017
Rintasyöpä on johtava naisten syöpäkuolemien aiheuttaja maailmanlaajuisesti. Luun morfogeneettiset proteiinit (BMP) ovat tranformoiva kasvutekijä beta- superperheeseen kuuluvia proteiineja. BMP:den tiedetään olevan osallisina monissa syöpätyypeissä. Rintasyövässä BMP4:llä on merkittäviä vaikutuksia solukasvuun, liikkuvuuteen ja levinnäisyyteen. Aiempien tutkimusten mukaan BMP4:n on havaittu laskevan solukasvua merkittävästi rintasyöpäsoluilla. Tämän tutkimuksen kohteena oli kaksi BMP4:n kohdegeeniä, ID2 (Inhibitor of DNA binding 2) ja SKIL eli SKI-like proto-onkogeeni. Hypoteesina oli selvittää johtuuko BMP4:n vaikutukset solukasvuun ID2- tai SKIL-geenistä. BT-474 ja T-47D rintasyöpäsoluja …
The Advances and Challenges of Liposome-Assisted Drug Release in the Presence of Serum Albumin Molecules: The Influence of Surrounding pH
2022
The aim of this study is to prepare a liposomal delivery system for 5-methyl-12 (H)-quino[3,4-b]-1,4-benzothiazine chloride (5-MBT) and study the in vitro release characteristics. The release of 5-MBT from a liposomal complex with human serum albumin (HSA) [LDPPC/5-MBT]:HSA was examined using the spectrophotometric method and differential scanning calorimetry (DSC). Electronic paramagnetic resonance was used to assess the influence of the pH of the environment on the conformation of phospholipids, the latter determining the degree of release of the encapsulated compound. The applied mathematical models made it possible to determine the necessary analytical parameters to facilitate the proce…