Search results for "Embryos"

Los contratos en materia de reproducción humana asistida: especial tratamiento de la autonomía de la voluntad en las donaciones de gametos y en el de…

2022

RESUMEN: A pesar de que en nuestro país la autonomía privada preside la regulación principal en materia de contratos, ésta se encuentra ampliamente condicionada a la hora de suscribir acuerdos en materia de reproducción humana asistida. En consecuencia, es la Ley 14/2006, de 26 de mayo, la que establece ineludiblemente las pautas y requisitos que deben cumplir las partes intervinientes. De esta forma, las donaciones de gametos llevadas a cabo en nuestro país, o la toma de decisión sobre el destino que habrá de darse a los embriones crioconservados, están absolutamente marcados por la ley, dejando un escaso margen de actuación a las clínicas y usuarias que participan en estas prácticas. Por …

Toxic effects induced by vanadium on sea urchin embryos

2020

Vanadium, a naturally occurring element widely distributed in soil, water and air, has received considerable interest because its compounds are often used in different applications, from industry to medicine. While the possible medical use of vanadium compounds is promising, its potential harmful effects on living organisms are still unclear. Here, for the first time, we provide a toxicological profile induced by vanadium on Paracentrotus lividus sea urchin embryos, reporting an integrated and comparative analysis of the detected effects reflecting vanadium-toxicity. At the morphological level we found a dose-dependent induction of altered phenotypes and of skeletal malformations. At the mo…

CiliaCarta: An integrated and validated compendium of ciliary genes

2019

The cilium is an essential organelle at the surface of mammalian cells whose dysfunction causes a wide range of genetic diseases collectively called ciliopathies. The current rate at which new ciliopathy genes are identified suggests that many ciliary components remain undiscovered. We generated and rigorously analyzed genomic, proteomic, transcriptomic and evolutionary data and systematically integrated these using Bayesian statistics into a predictive score for ciliary function. This resulted in 285 candidate ciliary genes. We generated independent experimental evidence of ciliary associations for 24 out of 36 analyzed candidate proteins using multiple cell and animal model systems (mouse…

Partial purification and some properties of a nucleoside phosphotransferase of chick embryos.

1978

A nucleoside phosphotransferase purified about 40fold from chick embryos utilizes efficiently as phosphate donors deoxyribonucleoside and pyrimidine ribonucleoside monophosphates, whereas the pyrimidine deoxyribonucleoside appear to be the preferred acceptors of phosphate. The enzyme is very unstable to heat, dilution and dialysis. A marked enhancement in the stability is caused by nucleotides and it seems associated with the formation of an aggregated state of the protein.

Evaluación de las células de la blastema de aleta caudal como donantes en quimeras intraespecíficas de pez cebra

2008

The blastema is a regenerative tissue with remarkable pluripotency. The aim of this work done on zebrafish (Danio rerio) was to define technical procedures required for obtaining and integrating blastema cells into embryos at the mid blastula transition stage (MBT) and the effect on survival, as well as the capacity to produce pigmented chimaeras. Wild type blastema cells were injected into gold type MBT embryos (E). Wild MBT blastomere cells were also injected into gold type MBT embryos as a control (C1). A second control group, C2, was not subjected to any manipulation. Survival was evaluated at 24, 48 and 72 h after performing the chimaerism, and the rate of adult chimaeras evaluated. Th…

Strategie di difesa attivate in risposta a stress, in embrioni di Paracentrotus lividus

2012

Sea urchin embryos are able to activate different defense strategies against stress. This model system allows to investigate numerous phenomena in multipotent cells, which interact among themselves, in their natural position, bypassing the disadvantages of isolated cells, deprived of their normal network. Cadmium (Cd) treatment triggers the accumulation of metal in embryonic cells and the activation of defense systems depending on concentration and exposure time, through the synthesis of heat shock proteins and/or the initiation of apoptosis. Here we show that Paracentrotus lividus embryos exposed to subacute/sublethal concentrations of Cd adopt autophagy as an additional stratagem to safeg…

Gametic embryogenesis through isolated microspore culture in mandarin (Citrus reticulata Blanco), Mandarino Tardivo Di Ciaculli: effect of meta-Topol…

2016

Haploid technology, allowing the single-step development of complete homozygous genotypes from heterozygous plants through gametic embryogenesis, has already an enormous impact on breeding programs of many important crops. Microspore embryogenesis can be carried out through in vitro culture of anther or isolated microspore. Experiments have been carried out via isolated microspore culture in Citrus reticulata (Blanco), Mandarino Tardivo di Ciaculli, investigating the influence of two culture media, of two plant growth regulator types and of two temperature treatments applied before the culture or just after the culture. After ten months of culture, for all the media tested, different struct…

Stress induced and physiological apoptosis during early development of sea urchin Paracentrotus lividus.

2008

Differences beetween physiological and induced apoptosis in sea urchin embryos.

2008

Perspectives of the encapsulation technology in the nursery activity of Citrus

2007

The genetic and phytosanitary certification has to guarantee the quality of the propagation material also in Citrus. Traditional methods can be assisted by the techniques of in vitro culture, which are fundamental for the mass production of high quality plants. The encapsulation technology represents an innovative tool to combine the advantages of micropropagation with handling ease, storability, reduced size of the propagules, mechanization potentiality, transportability of the gamic seeds. In this work somatic embryos obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (Citrus reticulata Blanco) were encapsulated in a sodium alginate matrix and synthetic se…