Search results for "Enos"
showing 10 items of 1576 documents
2014
Nitric oxide (NO) derived from the endothelial NO synthase (eNOS) has antihypertensive, antithrombotic, anti-atherosclerotic and antiobesogenic properties. Resveratrol is a polyphenol phytoalexin with multiple cardiovascular and metabolic effects. Part of the beneficial effects of resveratrol are mediated by eNOS. Resveratrol stimulates NO production from eNOS by a number of mechanisms, including upregulation of eNOS expression, stimulation of eNOS enzymatic activity and reversal of eNOS uncoupling. In addition, by reducing oxidative stress, resveratrol prevents oxidative NO inactivation by superoxide thereby enhancing NO bioavailability. Molecular pathways underlying these effects of resve…
Higher plants possess two different types of ATX1-like copper chaperones.
2007
Abstract Copper (Cu) chaperones constitute a family of small Cu+-binding proteins required for Cu homeostasis in eukaryotes. The ATX1 family of Cu chaperones specifically delivers Cu to heavy metal P-type ATPases. The plant Arabidopsis thaliana expresses the ATX1-like Cu chaperone CCH, which exhibits a plant-specific carboxy-terminal domain (CTD) with unique structural properties. We show that CCH homologues from other higher plants contain CTDs with structural properties similar to Arabidopsis CCH. Furthermore, we identify a new ATX1-like Cu chaperone in Arabidopsis, AtATX1, which functionally complements yeast atx1Δ and sod1Δ associated phenotypes, and localizes to the cytosol of Arabidop…
The role of mitochondria in sterigmatocystin-induced apoptosis on SH-SY5Y cells
2020
Mitochondria are cellular organelles involved in many crucial functions, such as generation of energy (ATP) and initiation of apoptosis. The aim of the present study was to evaluate the role of mitochondria in the toxicity induced by sterigmatocystin (STE), a mycotoxin produced by fungi of the genus Aspergillus, on SH-SY5Y cells. Our results showed that STE exposure decreased cell viability in a time- and concentration-dependent manner by MTT assay and caused mitochondrial dysfunction, as highlighted by the increase of STE cytotoxicity in cells forced to rely on mitochondrial oxidative phosphorylation. Furthermore, intracellular ATP depletion and increased mitochondrial reactive oxygen spec…
Prolactin supplementation to culture medium improves beta-cell survival
2010
OBJECTIVES.: Recent studies demonstrated that prolactin (PRL) has beneficial effects on β cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. MATERIALS AND METHODS.: Each human islet isolated from 14 deceased multiorgan donors was cultured in Miami modified media-1 supplemented with or without rhPRL (500 μg/L) for 48 hr. β-Cell survival and proliferation (BrdU and Ki-67) were determined by laser scanning cytometry. The cytoprotective effects of rhPRL against noxious stimuli were assessed by flow cytometry (tetramethylrhodamine ethyl ester…
In vitro effects of estradiol, testosterone, and progesterone on 5-methoxyindole content, cyclic adenosine 3',5'-monophosphate synthesis, and norepin…
1986
To examine the effects of estradiol, testosterone, or progesterone on cyclic adenosine 3',5'-monophosphate (AMP) accumulation, 5-methoxyindole levels, and norepinephrine (NE) release by the female guinea pig pineal complex, samples of the deep, intermediate, or superficial portions of the complex were incubated in vitro with varied concentrations of either hormone. Exposure for 10 minutes to physiological amounts of estradiol (10 nM) or to 100 microM NE increased significantly cyclic AMP levels to the same extent in the three pineal regions. A maximal effect on cyclic AMP accumulation was observed at 100-nM concentrations of estradiol, with a tendency to return to basal levels at 1-10 micro…
Circadian rhythm and effects of light on cAMP content of the dwarf hamster suprachiasmatic nucleus
1998
The present study was conducted in the dwarf hamster (Phodopus sungorus) to investigate whether a circadian rhythm is present in the content of the second messenger cyclic adenosine 3',5'-monophosphate (cAMP) in the suprachiasmatic nucleus (SCN), the endogenous clock in mammals. In animals held under light/dark conditions (LD), we observed high levels at the end of the light phase and low levels during the night in frozen SCN punches. In animals held in continuous dark, a similar rhythm was seen although a second peak was present in the subjective day. In senile hamsters under LD, the decrease of cAMP levels at the light transition was not seen. These data, obtained for the first time from …
Clinical Relevance of Thyroid-Stimulating Immunoglobulins in Graves' Ophthalmopathy
2011
Purpose Thyroid-stimulating immunoglobulins (TSIs) likely mediate Graves' ophthalmopathy (GO). The clinical relevance of these functional autoantibodies was assessed in GO. Design Cross-sectional trial. Participants A total of 108 untreated patients with GO. Methods Thyroid-stimulating immunoglobulins, assessed with a novel bioassay, bind to the thyrotropin receptor (TSHR) and transmit signals for cyclic adenosine monophosphate (cAMP)-dependent activation of luciferase gene expression. The cAMP/cAMP response element-binding protein/cAMP-regulatory element complex induces luciferase that is quantified after cell lysis. The TSI levels were correlated with activity and severity of GO and compa…
Regulación por estradiol de la producción de Angiotensina 1-7 y papel del receptor Mas en la producción de óxido nítrico en células endoteliales huma…
2017
El sistema renina-angiotensina intracelular (SRA) está formado por un conjunto de enzimas y metabolitos que se han relacionado con la regulación de la función cardiovascular y con la fisiopatología de la hipertensión arterial. El SRA clásico o circulante interviene en la regulación vascular principalmente con los péptidos bioactivos angiotensina (Ang) I y Ang II. La enzima que regula el paso de Ang I a Ang II es la enzima de conversión de la angiotensina 1 (ECA-1, la primera que se describió). En el año 2000 se descubrió que la enzima de conversión de la angiotensina 2 (ECA-2) convertía la Ang II en Ang 1-7. La Ang 1-7 es una molécula del SRA que tiene efecto fundamentalmente vasodilatador.…
Trattamento riabilitativo nella rottura traumatica del tendine quadricipitale nella malattia di Pompe
2013
La glicogenosi di tipo II o malattia di Pompe è una rara miopatia metabolica a trasmissione A.R., causata da mutazioni nel gene che codifica l'enzima lisosomiale alfa-glucosidasi acida, catalizzatore del processo di degradazione del glicogeno a glucosio. Presso l'U.O.C. di riabilitazione dell'A.O.U.P Paolo Giaccone giungeva nel marzo 2013 il signor B.G, di anni 52 con diagnosi di "postumi di intervento chirurgico di riparazione del tendine quadricipitale destro", per sottoporsi a trattamento riabilitativo in regime di DH; il paziente era affetto a glicogenosi metabolica di tipo II, diagnosticata nel 2007. nel nostro paziente l'esercizio terapeutico mirato al recupero del ROM e del tonotrofi…
Estudio de las alteraciones del esmalte en la enfermedad celiaca
2013
La enfermedad celiaca (EC) es una enteropatía caracterizada por una intolerancia permanente al gluten, proteína existente en el trigo, centeno, cebada y avena que provoca una lesión severa de las vellosidades intestinales, y se normaliza tras su retirada de la dieta. La sintomatología varía de un paciente a otro dependiendo del daño de la mucosa intestinal. La presentación típica incluye diarrea, pérdida de peso, fatiga, distensión abdominal, vómitos, deficiencias nutricionales y anemia por deficiencia de hierro. La hipoplasia del esmalte es el defecto más común en el desarrollo y mineralización de la dentición humana. Diferentes autores han descrito una mayor prevalencia de estas lesiones …