Search results for "Eryt"

showing 10 items of 966 documents

ProC® Global Assay in the Evaluation of Women with History of Severe Preeclampsia or HELLP Syndrome

2003

Preeclampsia/HELLP syndrome has been associated with a high incidence of defects in the protein C pathway and increased anticardiolipin-antibodies/lupus anticoagulants. It is also apparent that thrombophilia is responsible for other pregnancy complications, such as recurrent spontaneous abortion, fetal growth restriction, intrauterine fetal death, and abruptio placentae. ProC® Global is a new global clotting assay designed to evaluate the abnormalities in the protein C anticoagulant pathway. It is based on the ability of endogenous activated protein C, generated by activation of protein C by Protac®, to prolong an activated partial thromboplastin time. A total of 61 patients with a history…

AdultHELLP Syndromemedicine.medical_specialtyHELLP syndrome030204 cardiovascular system & hematologyThrombophiliaSensitivity and SpecificityGastroenterologyProtein SPreeclampsia03 medical and health sciences0302 clinical medicinePre-EclampsiaPregnancyInternal medicinemedicineHumansThrombophilia030212 general & internal medicineBlood coagulation testSystemic lupus erythematosusmedicine.diagnostic_testbiologybusiness.industryHematologyGeneral Medicinemedicine.diseaseCase-Control StudiesImmunologybiology.proteinFemaleBlood Coagulation TestsReagent Kits DiagnosticbusinessBiomarkersProtein CProtein Cmedicine.drugPartial thromboplastin timeClinical and Applied Thrombosis/Hemostasis
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Bronchial reactivity and intracellular magnesium: a possible mechanism for the bronchodilating effects of magnesium in asthma

1998

1.Increased bronchial smooth muscle contractility with consequent bronchial hyperreactivity are characteristic physiopathological events of asthma. Since magnesium intervenes in calcium transport mechanisms and intracellular phosphorylation reactions, it constitutes an important determinant of the contraction/relaxation state of bronchial smooth muscle. In the present study we investigated the relationship between bronchial reactivity, assessed by methacholine-provocation test, and magnesium concentrations both at extracellular and intracellular levels measured by spectrophotometry. Twenty-two patients with mild-to-moderate asthma and 38 non-asthmatic subjects with allergic rhinitis (24 all…

AdultHypersensitivity ImmediateIntracellular FluidMalemedicine.medical_specialtyAllergyErythrocytesParietariachemistry.chemical_elementBronchial Provocation TestsBronchoconstrictor AgentsInternal medicinemedicineHumansMagnesiumMethacholine ChlorideAsthmaAnalysis of VariancebiologyMagnesiumbusiness.industryGeneral MedicineSmooth muscle contractionbiology.organism_classificationmedicine.diseaseAsthmaBronchodilatationEndocrinologychemistryBronchial hyperresponsivenessImmunologyPollenFemaleBronchial HyperreactivitybusinessIntracellularClinical Science
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Increased protein kinase A regulatory subunit content and cGMP binding in erythrocyte membranes in liver cirrhosis

2003

Abstract Background/Aims : Patients with liver disease show increased plasma cGMP and decreased intracellular cGMP in lymphocytes. The initial aim of this work was to assess whether decreased intracellular cGMP and increased plasma cGMP may be due to increased ATP-dependent release of cGMP from cells. The results obtained led to a new aim: to identify and quantify a protein responsible for the increased cGMP binding found in erythrocyte membranes from patients with liver disease. Methods : ATP-dependent cGMP transport was determined in inside-out vesicles from erythrocyte membranes. cGMP-binding proteins were isolated from the membranes and identified by MALDI-TOF peptide mass fingerprint. …

AdultLiver CirrhosisMalemedicine.medical_specialtyProtein subunitPhosphodiesterase 3Biological Transport ActiveIn Vitro TechniquesBiologyInternal medicinemedicineHumansProtein kinase ACyclic GMPAgedCGMP bindingHepatologyErythrocyte MembraneMiddle AgedCyclic AMP-Dependent Protein KinasesMolecular WeightKineticsProtein SubunitsEndocrinologyCase-Control StudiesSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationCGMP transportbiology.proteinFemaleProtein AcGMP-dependent protein kinaseIntracellularJournal of Hepatology
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Candidatus Neoehrlichia mikurensis and Borrelia burgdorferi sensu lato detected in the blood of Norwegian patients with erythema migrans

2017

The most common tick-borne human disease in Norway is Lyme borreliosis. Ticks in Norway also harbour less known disease-causing agents such as Candidatus Neoehrlichia mikurensis, Borrelia miyamotoi and Rickettsia helvetica. However, human infections caused by these pathogens have never been described in Norway. The main aims of the study were to evaluate the contribution of several tick-borne bacterial agents, other than Borrelia burgdorferi sensu lato, to zoonotic diseases in Norway and to determine their clinical pictures. Blood samples from 70 symptomatic tick-bitten adults from the Agder counties in southern Norway were screened for seven tick-borne pathogens by using a commercial multi…

AdultMale0301 basic medicineBartonella030231 tropical medicine030106 microbiologyBorrelia miyamotoimedicine.disease_causeMicrobiologyMicrobiologyYoung Adult03 medical and health sciences0302 clinical medicineBorrelia burgdorferi GroupSeroepidemiologic StudiesPrevalencemedicineHumansBorrelia burgdorferiAgedTick-borne diseasebiologyNorwaySequence Analysis DNAMiddle Agedbacterial infections and mycosesbiology.organism_classificationmedicine.diseaseCoxiella burnetiiAnaplasma phagocytophilumVirologySpotted feverAnaplasmataceaeRNA BacterialInfectious DiseasesRickettsia helveticaRNA RibosomalInsect ScienceAnaplasmataceae InfectionsErythema Chronicum MigransbacteriaFemaleParasitologyTicks and Tick-borne Diseases
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Topical azithromycin for the prevention of Lyme borreliosis: a randomised, placebo-controlled, phase 3 efficacy trial

2017

Summary Background Lyme borreliosis develops in 1–5% of individuals bitten by ticks, but with a diagnostic gap affecting up to 30% of patients, a broadly applicable pharmacological prevention strategy is needed. Topical azithromycin effectively eradicated Borrelia burgdorferi sensu lato from the skin in preclinical studies. We assessed its efficacy in human beings. Methods In this randomised, double-blind, placebo-controlled, multicentre trial done in 28 study sites in Germany and Austria, adults were equally assigned to receive topical 10% azithromycin or placebo twice daily for 3 consecutive days, within 72 h of a tick bite being confirmed. Randomisation numbers, which were stratified by …

AdultMale0301 basic medicinemedicine.medical_specialty030106 microbiologyPopulationAzithromycinAzithromycinPlacebolaw.invention03 medical and health sciencesTicksDouble-Blind MethodRandomized controlled triallawGermanyInternal medicinemedicineClinical endpointAnimalsHumansTreatment FailureSeroconversioneducationLyme Diseaseeducation.field_of_studybusiness.industryAntibiotic ProphylaxisMiddle Agedbacterial infections and mycosesAnti-Bacterial AgentsSurgeryClinical trialInfectious DiseasesBorrelia burgdorferiErythema migransFemalebusinessmedicine.drugThe Lancet Infectious Diseases
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The relations of quality of life in patients with lupus erythematosus: Regression models versus qualitative comparative analysis

2019

Lupus erythematosus (LE) is an autoimmune disease that can have negative repercussions on the quality of life (QL) of those who suffer from it. The QL can be influenced by socio-demographic factors are related to the disease itself.To analyse the associative power of socio-demographic (age and sex) and medical variables (type of lupus, diagnostic time and symptomatically associated with lupus) on the QL in patients with lupus by comparing linear relations models and models based on comparative qualitative fuzzy analysis.This is a retrospective cross-sectional design.The sample consisted of 161 patients (88.4% female) diagnosed with LE between the ages of 18-66 years.Data were collected betw…

AdultMaleAdolescentDiseaseYoung Adult03 medical and health sciences0302 clinical medicineRisk groupsFuzzy LogicQuality of lifeHumansLupus Erythematosus SystemicMedicineIn patient030212 general & internal medicineQualitative ResearchGeneral NursingAgedRetrospective StudiesSystemic lupus erythematosusLupus erythematosus030504 nursingbusiness.industryQualitative comparative analysisRegression analysisMiddle Agedmedicine.diseaseCross-Sectional StudiesQuality of LifeRegression AnalysisFemale0305 other medical sciencebusinessClinical psychologyJournal of Advanced Nursing
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Efficacy of Rapamycin as Inducer of Hb F in Primary Erythroid Cultures from Sickle Cell Disease and β-Thalassemia Patients.

2015

Phenotypic improvement of hemoglobinopathies such as sickle cell disease and β-thalassemia (β-thal) has been shown in patients with high levels of Hb F. Among the drugs proposed to increase Hb F production, hydroxyurea (HU) is currently the only one proven to improve the clinical course of these diseases. However, Hb F increase and patient's response are highly variable, indicating that new pharmacological agents could be useful for patients not responding to HU or showing a reduction of response during long-term therapy. In this study we evaluated the efficacy of rapamycin, a lypophilic macrolide used for the prevention of acute rejection in renal transplant recipients, as an inducer of Hb…

AdultMaleAdolescentGenotypeThalassemiaClinical BiochemistryCellDiseaseAnemia Sickle Cellbeta-GlobinsPharmacologyBiologyYoung Adultalpha-GlobinsIn vivomedicineHumansHydroxyureaInducergamma-GlobinsGenetics (clinical)Cells CulturedFetal HemoglobinAgedErythroid Precursor CellsSirolimusBiochemistry (medical)beta-ThalassemiaClinical courseHematologyMiddle Agedmedicine.diseasePhenotypeMolecular biologyIn vitromedicine.anatomical_structureGene Expression RegulationMutationFemaleHemoglobin
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Fatigue in SLE: diagnostic and pathogenic impact of anti-N-methyl-D-aspartate receptor (NMDAR) autoantibodies.

2019

ObjectivesWe explored the impact of circulating anti-N-methyl-D-aspartate receptor (NMDAR) antibodies on the severity of fatigue in patients with systemic lupus erythematosus (SLE).MethodsSerum samples of 426 patients with SLE were analysed for the presence of antibodies to the NR2 subunit of the NMDAR. In parallel, the severity of fatigue was determined according to the Fatigue Scale for Motor and Cognitive functions questionnaire. In a subgroup of patients with SLE, the hippocampal volume was correlated with the levels of anti-NR2 antibodies. Isolated immunoglobulin G from patients with anti-NR2 antibodies were used for murine immunohistochemical experiments and functional assays on neuro…

AdultMaleAdolescentImmunologyEnzyme-Linked Immunosorbent AssayAntibodies Monoclonal HumanizedReceptors N-Methyl-D-AspartateSeverity of Illness IndexGeneral Biochemistry Genetics and Molecular BiologyImmunoglobulin GCell Line03 medical and health sciencesYoung Adult0302 clinical medicineCerebrospinal fluidRheumatologymedicineImmunology and AllergyHumansLupus Erythematosus SystemicReceptorFatigueAgedAutoantibodies030203 arthritis & rheumatologySystemic lupus erythematosusbiologybusiness.industryAutoantibodyMiddle Agedmedicine.diseaseBelimumabImmunologybiology.proteinImmunohistochemistryFemaleAntibodybusiness030217 neurology & neurosurgeryImmunosuppressive Agentsmedicine.drugAnnals of the rheumatic diseases
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Interferon-α Abrogates the Suppressive Effect of Apoptotic Cells on Dendritic Cells in anIn VitroModel of Systemic Lupus Erythematosus Pathogenesis

2013

Objective.An increased incidence of apoptotic cells and an increased activation of dendritic cells (DC) may be involved in the pathogenesis of systemic lupus erythematosus (SLE). We investigated the characteristics of apoptotic neutrophils and monocyte-derived DC of patients with SLE, their interaction, and the influence of autoantibodies and inflammatory cytokines on this interaction.Methods.Kinetics of neutrophil apoptosis and DC activation were studied by flow cytometry. To analyze the interaction of apoptotic cells with phagocytes, crossover coculture experiments were performed with DC from patients with SLE and apoptotic Jurkat T cells as well as with apoptotic neutrophils from patient…

AdultMaleAdolescentInterleukin-1betaImmunologyAlpha interferonApoptosisJurkat cellsProinflammatory cytokineJurkat CellsRheumatologyInterferonHumansLupus Erythematosus SystemicImmunology and AllergyMedicineAgedAutoantibodiesU937 cellbusiness.industryInterleukin-17Interferon-alphaDendritic CellsU937 CellsMiddle AgedApoptosisImmunologyFemaleCytokine secretionInterleukin 17businessmedicine.drugThe Journal of Rheumatology
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Prediction of the hemoglobin level in hemodialysis patients using machine learning techniques

2013

HighlightsDifferent prediction algorithms were used to predict Hb levels in CRF patients.Prediction errors in the validation cohorts of patients were around 0.6g/dl.Difficulty to obtain lower errors due to the measuring machine precision (0.2g/dl).Relevance analysis of features have been applied for each predictor. Patients who suffer from chronic renal failure (CRF) tend to suffer from an associated anemia as well. Therefore, it is essential to know the hemoglobin (Hb) levels in these patients. The aim of this paper is to predict the hemoglobin (Hb) value using a database of European hemodialysis patients provided by Fresenius Medical Care (FMC) for improving the treatment of this kind of …

AdultMaleAdolescentmedicine.medical_treatmentHealth InformaticsMachine learningcomputer.software_genreDisease clusterSensitivity and SpecificityHemoglobinsYoung AdultArtificial IntelligenceRenal DialysismedicineHumansComputer SimulationCluster analysisErythropoietinAgedAged 80 and overDose-Response Relationship DrugArtificial neural networkbusiness.industryModels CardiovascularLinear modelReproducibility of ResultsAnemiaMiddle AgedRegressionDrug Therapy Computer-AssistedComputer Science ApplicationsSupport vector machineTreatment OutcomeAdaptive resonance theoryFemaleHemodialysisArtificial intelligenceDrug MonitoringbusinesscomputerAlgorithmsBiomarkersSoftwareComputer Methods and Programs in Biomedicine
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