Search results for "Exon"

showing 10 items of 437 documents

Genomic organization and promoter characterization of the gene encoding a putative endoplasmic reticulum chaperone, ERp29

2002

Abstract ERp29 is a soluble protein localized in the endoplasmic reticulum (ER) of eukaryotic cells, which is conserved in all mammalian species. The N-terminal domain of ERp29 displays sequence and structural similarity to the protein disulfide isomerase despite the lack of the characteristic double cysteine motif. Although the exact function of ERp29 is not yet known, it was hypothesized that it may facilitate folding and/or export of secretory proteins in/from the ER. ERp29 is induced by ER stress, i.e. accumulation of unfolded proteins in the ER. To gain an insight into the mechanisms regulating ERp29 expression we have cloned and characterized the rat ERp29 gene and studied in details …

5' Flanking RegionRecombinant Fusion ProteinsMolecular Sequence DataCHO CellsBiologyCell LineMiceCricetinaeSequence Homology Nucleic AcidGene expressionTumor Cells CulturedGeneticsAnimalsHumansRNA MessengerLuciferasesPromoter Regions GeneticProtein disulfide-isomeraseGeneHeat-Shock ProteinsPhylogenyBase SequenceGene Expression ProfilingEndoplasmic reticulumPromoter3T3 CellsDNAExonsSequence Analysis DNAGeneral MedicineMolecular biologyIntronsRatsHousekeeping geneSecretory proteinGenesUnfolded protein responseFemaleTranscription Initiation SiteSequence AlignmentHeLa CellsGene
researchProduct

Sequence of a sea urchin hsp70 gene and its 5' flanking region.

1990

We report the nucleotide sequence of a 4470-bp fragment derived from a sea urchin genomic clone containing part of a heat-shock protein 70 (Hsp70)-encoding gene. This fragment, named hsp70 gene II, contains 1271 bp of the flanking region and 3299 bp of structural gene sequence interrupted by five introns and encoding the N-terminal 371 amino acids (aa) of the protein. The 5' flanking region contains a putative TATA element, two CCAAT boxes, four heat-shock consensus sequence elements (hse) and one consensus sequence for binding of Sp1. Remarkable homologies were observed for deduced aa sequence and intron-exon organization between hsp70 gene II and rat hsc73 gene.

5' flanking regionMolecular Sequence DataRestriction MappingBiologyExonSequence Homology Nucleic AcidConsensus SequenceGeneticsConsensus sequenceAnimalsAmino Acid SequencePromoter Regions GeneticGenePeptide sequenceHeat-Shock ProteinsGeneticsBase SequenceStructural geneNucleic acid sequenceGeneral MedicineExonsMolecular biologyIntronsGenesRegulatory sequenceSea UrchinsGene
researchProduct

A computational method for studying the relation between alternative splicing and DNA methylation.

2015

Alternative splicing is an important mechanism in eukaryotes that expands the transcriptome and proteome significantly. It plays an important role in a number of biological processes. Understanding its regulation is hence an important challenge. Recently, increasing evidence has been collected that supports an involvement of intragenic DNA methylation in the regulation of alternative splicing. The exact mechanisms of regulation, however, are largely unknown, and speculated to be complex: different methylation profiles might exist, each of which could be associated with a different regulation mechanism. We present a computational technique that is able to determine such stable methylation pa…

7Alternative SplicingHumansRNAMethods OnlineExonsDNA MethylationTranscriptomeIntronsSoftware24Epigenesis GeneticNucleic acids research
researchProduct

CCDC 705738: Experimental Crystal Structure Determination

2009

Related Article: D.Meshcheryakov, V.Bohmer, M.Bolte, V.Hubscher-Bruder, F.Arnaud-Neu|2009|Chem.-Eur.J.|15|4811|doi:10.1002/chem.200802573

97979898-Tetramethyl-3435191-tetra-t-butyl-153147637995-hexaoxa-6822243840545670728688-dodeca-azapentadecacyclo[91.3.1.14549.0596.0914.01621.02530.03237.04146.04853.05762.06469.07378.08085.08994]octanonaconta-1(96)2491113161820252729323436414345485052575961646668737577808284899193-hexatriacontaene-72339557187-hexone acetonitrile ethanol ethyl acetate solvate monohydrateSpace GroupCrystallographyCrystal SystemCrystal StructureCell ParametersExperimental 3D Coordinates
researchProduct

Las cláusulas abusivas en las hojas de encargo de servicios jurídicos: en especial, la cláusula de exoneración y limitación de responsabilidad del pr…

2022

El trabajo tiene como objetivo exponer el régimen jurídico aplicable a las hojas de encargo profesional suscritas entre un abogado y su cliente, para con ese antecedente analizar las principales cláusulas que son declaradas abusivas por los tribunales, en concreto, la relativa al precio del contrato, a la facultad de desistimiento y, en especial, a la cláusula de exoneración o limitación de responsabilidad.

:CIENCIAS JURÍDICAS Y DERECHO [UNESCO]exoneración de responsabilidadUNESCO::CIENCIAS JURÍDICAS Y DERECHOcláusulas abusivas
researchProduct

Los programas de cumplimiento penal : Aspectos generales

2021

In Spain, as in many other countries, corporate criminal liability has been a reality for years, as well as criminal compliance programs that allow them to be mitigated or exonerated from liability. In this article we will present some of the main keys to consider for the implementation of these mechanisms. This is a series of general considerations that can serve as a guide to understand the complex system of crime prevention provided by the organization and management models in the corporate environment.

:CIENCIAS JURÍDICAS [UNESCO]responsabilidad penal de las personas jurídicas.self-regulationJosé In Spain2070-8157 22082 Revista Boliviana de Derecho 565487 2021 31 7730062 Los programas de cumplimiento penal Aspectos generales León Alapontas in many other countriesComputingMilieux_LEGALASPECTSOFCOMPUTINGCriminal compliance programsautorregulaciónUNESCO::CIENCIAS JURÍDICASas well as criminal compliance programs that allow them to be mitigated or exonerated from liability. In this article we will present some of the main keys to consider for the implementation of these mechanisms. This is a series of general considerations that can serve as a guide to understand the complex system of crime prevention provided by the organization and management models in the corporate environment. Programas de cumplimiento penalcorporate criminal liability 354 389corporate criminal liability has been a reality for years
researchProduct

Non-pheochromocytoma (PCC)/paraganglioma (PGL) tumors in patients with succinate dehydrogenase-related PCC-PGL syndromes: a clinicopathological and m…

2014

ObjectiveAlthough the succinate dehydrogenase (SDH)-related tumor spectrum has been recently expanded, there are only rare reports of non-pheochromocytoma/paraganglioma tumors in SDHx-mutated patients. Therefore, questions still remain unresolved concerning the aforementioned tumors with regard to their pathogenesis, clinicopathological phenotype, and even causal relatedness to SDHx mutations. Absence of SDHB expression in tumors derived from tissues susceptible to SDH deficiency is not fully elucidated.Design and methodsThree unrelated SDHD patients, two with pituitary adenoma (PA) and one with papillary thyroid carcinoma (PTC), and three SDHB patients affected by renal cell carcinomas (RC…

AdenomaAdultMalemedicine.medical_specialtyPathologySDHBEndocrinology Diabetes and MetabolismSDHALoss of HeterozygosityBiologyPheochromocytomaLoss of heterozygosityEndocrinologyParagangliomaInternal medicinemedicineHumansPituitary NeoplasmsThyroid NeoplasmsCarcinoma Renal CellGerm-Line MutationCarcinomaGeneral MedicineExonsMiddle Agedmedicine.diseaseNeuroblastic TumorCarcinoma Papillary3. Good healthNeoplasm ProteinsSuccinate DehydrogenaseEndocrinologyThyroid Cancer PapillaryMutationFemaleSDHDClear cellGene DeletionEuropean Journal of Endocrinology
researchProduct

Exome Sequencing Reveals VCP Mutations as a Cause of Familial ALS

2010

Summary Using exome sequencing, we identified a p.R191Q amino acid change in the valosin-containing protein ( VCP ) gene in an Italian family with autosomal dominantly inherited amyotrophic lateral sclerosis (ALS). Mutations in VCP have previously been identified in families with Inclusion Body Myopathy, Paget disease, and Frontotemporal Dementia (IBMPFD). Screening of VCP in a cohort of 210 familial ALS cases and 78 autopsy-proven ALS cases identified four additional mutations including a p.R155H mutation in a pathologically proven case of ALS. VCP protein is essential for maturation of ubiquitin-containing autophagosomes, and mutant VCP toxicity is partially mediated through its effect on…

Adenosine TriphosphataseMaleCell Cycle ProteinsUBQLN2Cohort Studies0302 clinical medicineReference ValuesValosin Containing ProteinCell Cycle ProteinReference ValueAmyotrophic lateral sclerosisExome sequencingAdenosine TriphosphatasesGenetics0303 health sciencesGeneral NeuroscienceExonsMiddle AgedPedigree3. Good healthMultisystem proteinopathyFemaleSettore MED/26 - NeurologiaCase-Control StudieChromosomes Human Pair 9HumanFrontotemporal dementiaNeuroscience(all)Valosin-containing proteinExonBiologyProtein degradationTARDBPArticle03 medical and health sciencesmedicineHumansAged030304 developmental biologyAmyotrophic lateral sclerosis familial ALS exome sequencingNeuroscience (all)business.industryAmyotrophic Lateral Sclerosismedicine.diseaseAmino Acid SubstitutionCase-Control StudiesMutationbiology.proteinCohort Studiebusiness030217 neurology & neurosurgeryAmyotrophic Lateral SclerosiNeuron
researchProduct

A recurrent TP63 mutation causing EEC3 and Rapp–Hodgkin syndromes

2016

The ectrodactyly, ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3; OMIM #604292), the Rapp-Hodgkin syndrome (RHS), and various other syndromes are caused by mutations in the TP63 gene, which encodes a p53-like transcription factor. Here, we report on a woman aged 37 years and her daughter aged 3 years with the previously reported c.1028G>A (p.Arg343Gln) mutation in exon 8 of TP63. The mother lacked ectrodactyly, indicating a diagnosis of RHS, whereas the girl presented with all three major features (ectrodactyly, ectodermal dysplasia, clefting) and different minor features (including small and brittle nails, and recurrent conjunctivitis believed to be because of stenotic and blo…

Adult0301 basic medicineHeterozygoteEctodermal dysplasiamedicine.medical_specialtyEctrodactylyFoot Deformities CongenitalCleft Lipmedia_common.quotation_subjectmedicine.disease_causePathology and Forensic MedicineFingers030207 dermatology & venereal diseases03 medical and health sciencesExon0302 clinical medicineEctodermal DysplasiaTP63medicineHumansAlleleAllelesGenetics (clinical)media_commonDaughterMutationbusiness.industryTumor Suppressor ProteinsFaciesExonsGeneral Medicinemedicine.diseaseDermatologyPenetrancePedigreeCleft PalatePhenotype030104 developmental biologyAmino Acid SubstitutionChild PreschoolMutationPediatrics Perinatology and Child HealthFemaleAnatomybusinessHand Deformities CongenitalTranscription FactorsClinical Dysmorphology
researchProduct

δ 1‐OPIOID receptor‐mediated controlofacetylcholine (ACh) release in human neocortex slices

1998

In slices of human neocortex, prelabelled with [3H]-choline, the release of [3H]-acetylcholine reflects the evoked release of endogenous acetylcholine which was elicited by the same electrical stimulation paradigm. [3H]-Acetylcholine release was depressed by the delta-opioid receptor agonist D-Pen2-D-Pen5-enkephalin. When the nerve endings were depolarized by elevating extracellular potassium the evoked [3H]-acetylcholine release was similarly depressed by D-Pen2-D-Pen5-enkephalin in the absence, but not in the presence, of tetrodotoxin which blocks action potential propagation. Therefore, the delta-opioid receptor inhibiting [3H]-acetylcholine release should not be located to cholinergic n…

AdultAgonistmedicine.medical_specialtymedicine.drug_classNarcotic AntagonistsNeocortexTetrodotoxinIn Vitro TechniquesOctreotideBenzylidene Compoundschemistry.chemical_compoundDevelopmental NeuroscienceInterneuronsOpioid receptorReceptors Opioid deltaInternal medicinemedicineHumansReceptorAgedAged 80 and overNeocortexEnkephalinsMiddle AgedReceptor antagonistAcetylcholineElectric StimulationNaltrexoneEndocrinologymedicine.anatomical_structurenervous systemchemistryTetrodotoxinCholinergicEnkephalin D-Penicillamine (25)-AcetylcholineDevelopmental Biologymedicine.drugInternational Journal of Developmental Neuroscience
researchProduct