Search results for "Expression"

Parathyroid Hormone Related Protein (PTHrP)-Associated Molecular Signatures in Tissue Differentiation and Non-Tumoral Diseases

2023

Commenting on poverty online : A corpus-assisted discourse study of the Suomi24 forum

2020

This paper brings new insight to poverty and social exclusion through an analysis of how poverty-related issues are commented on in the largest online discussion forum in Finland: Suomi24 (‘Finland24’). For data, we use 32,407 posts published in the forum in 2014 that contain the word köyhä (‘poor’) or a predefined semantically similar word. We apply the Corpus-Assisted Discourse Studies (CADS) method, which combines quantitative methods and qualitative discourse analysis. This methodological solution allows us to analyse both large-scale tendencies and detailed expressions and nuances on how poverty is discussed. The quantitative analysis is conducted with topic modelling, an unsupervised …

Different Modes of Regulation of the Expression of Dextransucrase in

2019

Leuconostoc lactis AV1 strain isolated from a Tunisian avocado was characterized as a dextran producer. The promoter PdsrLL and the dsrLL gene encoding the DsrLL dextransucrase responsible for the dextran synthesis were transcriptionally fused to the mCherry coding gene generating the pRCR20 plasmid. Upon plasmid transfer, both AV1n and the dextran non-producing Leuconostoc mesenteroides CM70 became red due to expression of the mCherry from the PdsrLL-dsr-mrfp transcriptional fusion. Characterization of the polymers present in cultures supernatants revealed that the DsrLL encoded from pRCR20 in the recombinant bacteria was able to synthesize dextran. The production of dextran by the DsrLL i…

Przebieg rozwoju mowy u dzieci z SLI-PE i LB – analiza porównawcza

2017

W niniejszym artykule prezentujemy fragment szeroko zakrojonych badań poświęconych problematyce dzieci z poważnym opóźnieniem w rozwoju mowy; opóźnieniem, którego nie można wiązać z żadnym uchwytnym czynnikiem etiologicznym (jak w wypadku dzieci z późnym rozkwitem mowy LB – ang. late bloomers, czy dzieci ze specyficznym zaburzeniem językowym – SLI, ang. specific language impairment). Postawienie trafnej diagnozy przed ukończeniem 3. r.ż. dziecka, tj odróżnienie zjawiska late bloomers od late talkers, czyli dzieci później rozkwitających pod względem językowym od później mówiących jest obecnie niemożliwe ze względu na brak doniesień z badań poświęconych dzieciom z LB i analiz porównawczych. P…

Risus abundat? Al confine tra libertà di espressione e violenza verbale

2022

The question that lies at the heart of this short essay concerns the effects of laughter on the relationship between the right to freedom of expression, on the one hand, and the potential exercise of verbal violence on the other. In short, the question we ask is whether the presence of laughter increases the spectrum of freedom of expression by reducing that of verbal violence, or, on the contrary, whether the very element of laughter does not laughter does not, on the contrary, determine a multiplication of violence in certain discursive situations.

The transcriptome analysis of Strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease.

2012

Background: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. Principal Findings: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037…

Genome-Wide Inhibition of Pro-atherogenic Gene Expression by Multi-STAT Targeting Compounds as a Novel Treatment Strategy of CVDs.

2018

Cardiovascular diseases (CVDs), including atherosclerosis, are globally the leading cause of death. Key factors contributing to onset and progression of atherosclerosis include the pro-inflammatory cytokines Interferon (IFN)a and IFN? and the Pattern Recognition Receptor (PRR) Toll-like receptor 4 (TLR4). Together, they trigger activation of Signal Transducer and Activator of Transcription (STAT)s. Searches for compounds targeting the pTyr-SH2 interaction area of STAT3, yielded many small molecules, including STATTIC and STX-0119. However, many of these inhibitors do not seem STAT3-specific. We hypothesized that multi-STAT-inhibitors that simultaneously block STAT1, STAT2, and STAT3 activit…

Azithromycin Differentially Alters TCR-Activated Helper T Cell Subset Phenotype and Effector Function

2020

In addition to their antibiotic activities, azithromycin (AZM) exhibits anti-inflammatory effects in various respiratory diseases. One of the potent anti-inflammatory mechanisms is through inhibition of CD4+ helper T (Th) cell effector function. However, their impact on specific Th subset is obscure. Herein, we demonstrate the cellular basis of phenotypic and functional alterations associated with Th subsets following AZM treatment in vitro. Using well-characterized Th subset specific chemokine receptors, we report significant suppression of T cell receptor (TCR)-stimulated hyperactivated CCR4+CXCR3+ (Th0) expansion compared to CCR4-CXCR3+ (Th1-like) and CCR4+CXCR3- (Th2-like) cells. Intere…

RUNX3 and T-Bet in Immunopathogenesis of Ankylosing Spondylitis—Novel Targets for Therapy?

2019

Susceptibility to ankylosing spondylitis (AS) is polygenic with more than 100 genes identified to date. These include HLA-B27 and the aminopeptidases (ERAP1, ERAP2, and LNPEPS), which are involved in antigen processing and presentation to T-cells, and several genes (IL23R, IL6R, STAT3, JAK2, IL1R1/2, IL12B, and IL7R) involved in IL23 driven pathways of inflammation. AS is also strongly associated with polymorphisms in two transcription factors, RUNX3 and T-bet (encoded by TBX21), which are important in T-cell development and function. The influence of these genes on the pathogenesis of AS and their potential for identifying drug targets is discussed here.

IFI16 expression is related to selected transcription factors during B-cell differentiation

2015

The interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulation during maturation of B- and T-cells. We explored the role of IFI16 in normal B-cell subsets by analysing its expression and relationship with the major transcription factors involved in germinal center (GC) development and plasma-cell (PC) maturation.IFI16mRNA was differentially expressed in B-cell subsets with significant decrease inIFI16mRNA in GC and PCs with respect to naïve and memory subs…