Search results for "Extender"

Kinematic Sub-Populations in Bull Spermatozoa: A Comparison of Classical and Bayesian Approaches

2020

The ejaculate is heterogenous and sperm sub-populations with different kinematic patterns can be identified in various species. Nevertheless, although these sub-populations are statistically well defined, the statistical differences are not always relevant. The aim of the present study was to characterize kinematic sub-populations in sperm from two bovine species, and diluted with different commercial extenders, and to determine the statistical relevance of sub-populations through Bayesian analysis. Semen from 10 bulls was evaluated after thawing. An ISAS&reg

Effect of diluent composition on the dynamics of sperm DNA fragmentation and other sperm quality parameters in ram during incubation at 37°C

2015

Abstract This study was conducted to assess the effect of different semen extenders on the dynamics of sperm DNA fragmentation (SDF) and other quality sperm parameters in ram. To this end, second ejaculates from 6 adult males were collected using an artificial vagina and diluted in either INRA-96 ® , milk, MOPS, TRIS, TES, HEPES, citrate or phosphate-based extenders. Semen samples were incubated at 37 °C and the SDF, motility and membrane integrity were assessed after 0, 3, 6 and 24 h intervals. Significantly higher SDF indexes were obtained at 6 and 24 h of incubation in the milk-based than in all the buffer-based extenders. At 24 h, sperm diluted in INRA also reached a higher SDF than MOP…

Development and validation of a 5 stroke engine for range extenders application

2014

International audience; A 5-stroke turbo-charged port-injection spark-ignition engine has been developed in the present study for use as a range extender or series-hybrid main power source. The development and the design of the engine are based on 0D/1D model and experimental results have been compared with the engine model. The 5-stroke engine is a three-cylinder in which two cylinders perform a four-stroke cycle and alternatively a second expansion of the burnt gases is performed in the third cylinder. The boost pressure delivered by the turbocharger is controlled by a particular innovative system called “smart wastegate”, different from a conventional wastegate, consisting in a variable …

Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage

2020

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of …

Combined effect of type and capture area of counting chamber and diluent on Holstein bull sperm kinematics

2018

The evaluation of sperm motion is crucial for processing of seminal doses for artificial insemination. Here, the combined effect of the type and capture area of three counting chambers, together with the type of diluent employed, on sperm motility was analysed. Ejaculates from thirteen Holstein bulls were used for sperm kinematic analysis with the ISAS® v1 CASA-Mot system, using two capillary-loaded counting chambers (Leja® and Cell-Vu® ) and one drop displacement chamber (Makler® ). Nine fixed positions were analysed per chamber type, considering central and lateral and three longitudinal fields. Independent of the diluent used, differences were found between the three chambers. Independen…

Effect of solid storage at 15 degrees C on the subsequent motility and fertility of rabbit semen.

2005

We conducted two studies to improve preservation of rabbit semen. The objective of the first study was determine whether a glucose- and fructose-based extender with two different amounts of gelatin would solidify at 15 degrees C, and to evaluate the influence of gelatin supplementation on sperm motility parameters after storing semen up to 10 days at 15 degrees C. The fertility of rabbit semen diluted in the best gelatin-supplemented extender established in Study 1 and stored for up to 5 days was evaluated in the second study. In Study 1, semen was collected with an artificial vagina from 40 bucks. Each ejaculate was diluted to (80-100) x 10(6) spermatozoa/mL (1:3, semen/extender) at 37 deg…

Effect of chain extender on the morphology, thermal, viscoelastic, and dielectric behavior of soybean polyurethane

2021

Use of new hydroxytelechelic cis-1,4-polyisoprene (HTPI) in the synthesis of polyurethanes (PUs): Influence of isocyanate and chain extender naturean…

2006

AbstractNew segmented polyurethanes (thermoplastic-elastomers) based on previously described hydroxytelechelic cis-1,4-polyisoprene (HTPI), miscellaneous isocyanates and chain extenders at various equivalent ratios were prepared by the classical one-shot method. The influence of the nature of isocyanate and chain extender, as well as their equivalent ratios, on the mechanical and thermal materials behavior was investigated. Thus, a comparative assessment of material properties was performed. Microphase separation of hard and soft segments was observed by DMTA, DSC and AFM. Moreover, according to Thermogravimetric Analysis (TGA), linear relationships depending on the isocyanate and chain ext…

TI: Cyclophosphazenes as polymer modifiers

2003

The utilization of cyclophosphazenes as polymer modifiers is reviewed, with particular concern to their exploitation as versatile chain extenders, possibly for recycle problems, crosslinkers, to enhance mechanical properties of polymeric materials, branchers, to selectively introduce ramifications in linear polymers, and compatibilizers, to favor the formation of blends between originally incompatible organic macromolecules. The great versatility of the synthetic methods put forward for these substrates, together with the ease of controlling their modification, functionalization and reactivity are important parameters for the evaluation of which type of use is more feasible for these trimer…

Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa

2021

Cooled preservation of semen is usually associated with artificial insemination and genetic improvement programs in livestock species. Several studies have reported an increase in reactive oxidative species and a decrease in antioxidant substances and sperm quality parameters during long-term semen storage at refrigerated temperatures. The supplementation of antioxidants in extenders before refrigeration could reduce this detrimental effect. Various antioxidants have been tested, both enzymatic, such as superoxide dismutase and catalase, and non-enzymatic, such as reduced glutathione, vitamins E and C and melatonin. However, the problem of oxidative stress in semen storage has not been full…