6533b7dbfe1ef96bd126f805

RESEARCH PRODUCT

Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage

Balma García-colomerM.a. SilvestreSara SadeghiErnesto A. GomezCarmen López-fernándezJesús YánizJaime GosálvezRaquel Del Gallego

subject

L53 Animal physiology - Reproductionmedicine.medical_treatmentDNA fragmentationmitochondrial activitylaw.invention0302 clinical medicineL10 Animal genetics and breedinglawlcsh:QH301-705.5Semen qualitySperm motilityreproductive and urinary physiologyLiquid Storageliquid storage030219 obstetrics & reproductive medicineMurciano-GranadinaExtenderSperm washingMotilityMitochondrial Activity04 agricultural and veterinary sciencesBiología y Biomedicina / BiologíamotilityDNA fragmentationGeneral Agricultural and Biological SciencesGenetic improvementendocrine systemMotilityShelf lifeSemen ExtendersSemenDNA FragmentationBiologyGeneral Biochemistry Genetics and Molecular BiologyAi (artificial insemination)ArticleAndrology03 medical and health sciencesSemenmedicineU10 Mathematical and statistical methodsgoat spermGeneral Immunology and Microbiologyurogenital systemArtificial insemination0402 animal and dairy scienceGoat Sperm040201 dairy & animal scienceSpermFrozen storagelcsh:Biology (General)

description

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of semen at different temperatures (5 &deg

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