0000000000368890

AUTHOR

Raquel Del Gallego

showing 2 related works from this author

Effect of sperm concentration and storage temperature on goat spermatozoa during liquid storage

2020

The use of cooled semen is relatively common in goats. There are a number of advantages of cooled semen doses, including easier handling of artificial insemination (AI) doses, transport, more AI doses per ejaculate, and higher fertility rates in comparison with frozen AI doses. However, cooled semen has a short shelf life. The objective of this study was to examine the effect of temperature and sperm concentration on the in vitro sperm quality during liquid storage for 48 h, including sperm motility and kinetics, response to oxidation, mitochondrial membrane potential (MMP) and DNA fragmentation in goats. Three experiments were performed. In the first, the effects of liquid preservation of …

L53 Animal physiology - Reproductionmedicine.medical_treatmentDNA fragmentationmitochondrial activitylaw.invention0302 clinical medicineL10 Animal genetics and breedinglawlcsh:QH301-705.5Semen qualitySperm motilityreproductive and urinary physiologyLiquid Storageliquid storage030219 obstetrics & reproductive medicineMurciano-GranadinaExtenderSperm washingMotilityMitochondrial Activity04 agricultural and veterinary sciencesBiología y Biomedicina / BiologíamotilityDNA fragmentationGeneral Agricultural and Biological SciencesGenetic improvementendocrine systemMotilityShelf lifeSemen ExtendersSemenDNA FragmentationBiologyGeneral Biochemistry Genetics and Molecular BiologyAi (artificial insemination)ArticleAndrology03 medical and health sciencesSemenmedicineU10 Mathematical and statistical methodsgoat spermGeneral Immunology and Microbiologyurogenital systemArtificial insemination0402 animal and dairy scienceGoat Sperm040201 dairy & animal scienceSpermFrozen storagelcsh:Biology (General)
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Novel noninvasive embryo selection algorithm combining time-lapse morphokinetics and oxidative status of the spent embryo culture medium.

2019

Objective To develop a noninvasive embryo selection algorithm consisting of time-lapse morphokinetics and the oxidative status of the spent embryo culture medium determined using the Thermochemiluminescence (TCL) Analyzer. Design Retrospective cohort. Setting Not applicable. Patient(s) From women participating in the oocyte donation program, data from 505 samples of spent embryo culture media samples from 292 intracytoplasmic sperm injection cycles. Intervention(s) None. Main Outcome Measure(s) Morphokinetic parameters assessed during incubation in the time-lapse system Embryoscope. Oxidative parameters (H1sm, H2sm, and H3sm) from the spent culture medium on day 5 of incubation measured usi…

0301 basic medicineAdultanimal structuresmedicine.medical_treatmentOxidative phosphorylationBiologyTime-Lapse ImagingIntracytoplasmic sperm injectionAndrologyCohort StudiesEmbryo Culture Techniques03 medical and health sciences0302 clinical medicineHuman fertilizationPregnancymedicineHumansIncubationRetrospective StudiesPregnancy030219 obstetrics & reproductive medicineObstetrics and GynecologyEmbryo cultureEmbryomedicine.diseaseEmbryo TransferCulture MediaKineticsOxidative Stress030104 developmental biologyReproductive Medicineembryonic structuresFemaleEmbryo qualityAlgorithmsFertility and sterility
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