Search results for "Extract"

Development of  a qPCR assay for specific quantification of Botrytis cinerea on grapes

2010

The aim of this study was to develop a system for rapid and accurate real-time quantitative PCR (qPCR) identification and quantification of Botrytis cinerea , one of the major pathogens present on grapes. The intergenic spacer (IGS) region of the nuclear ribosomal DNA was used to specifically detect and quantify B. cinerea . A standard curve was established to quantify this fungus. The qPCR reaction was based on the simultaneous detection of a specific IGS sequence and also contained an internal amplification control to compensate for variations in DNA extraction and the various compounds from grapes that inhibit PCR. In these conditions, the assay had high efficiency (97%), and the limit o…

Spent Coffee Grounds Extract, Rich in Mannooligosaccharides, Promotes a Healthier Gut Microbial Community in a Dose-Dependent Manner.

2019

Coffee is one of the most consumed beverages around the world, and as a consequence, spent coffee grounds are a massively produced residue that is causing environmental problems. Reusing them is a major focus of interest presently. We extracted mannooligosaccharides (MOS) from spent coffee grounds and submitted them to an in vitro fermentation with human feces. Results obtained suggest that MOS are able to exert a prebiotic effect on gut microbiota by stimulating the growth of some beneficial genera, such as Barnesiella, Odoribacter, Coprococcus, Butyricicoccus, Intestinimonas, Pseudoflavonifractor, and Veillonella. Moreover, short-chain fatty acids (SCFA) production also increased in a dos…

Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR

2011

Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…

A fast extraction procedure of 2-dodecylcyclobutanone for the identification of irradiated pork, employing gas chromatographic/mass spectrometric ana…

2008

Determination of 60 Migrant Substances in Plastic Food Contact Materials by Vortex-Assisted Liquid-Liquid Extraction and GC-Q-Orbitrap HRMS

2021

A GC-HRMS analytical method for the determination of 60 migrant substances, including aldehydes, ketones, phthalates and other plasticizers, phenol derivatives, acrylates, and methacrylates, in plastic food contact materials (FCM) has been developed and validated. The proposed method includes migration tests, according to Commission Regulation (EU) 10/2011, using four food simulants (A, B, C, and D1), followed by vortex-assisted liquid–liquid extraction (VA-LLE) and GC-Q-Orbitrap HRMS analysis in selected ion monitoring (SIM) mode, with a resolving power of 30,000 FWHM and a mass accuracy ≤5 ppm. The method was validated, showing satisfactory linearity (R2 ≥ 0.98 from 40 to 400 …

Effects of menthol on mouse gastric mechanical activity

2011

Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro

2011

Objective: The objective of this study was to evaluate the cytotoxicity of the plant extract ankaferd blood stopper (ABS) in vitro. Study Design: ABS was eluted with fresh Dulbecco's Modified Eagle's Medium (DMEM) without serum for 72 h, at 37°C. The cells treated with various dilutions of ABS were seeded into 96-well microplate at 10 4 /well in triplicates. Cells without treatment served as a control group. The number of viable cells after 48 h incubation was determined by a modified 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The relative viability of pulp cells was expressed as color intensity of the number in the experimental wells relative to that of t…

Binocular vision alterations after refractive and cataract surgery: a review.

2018

To review binocular and accommodative disorders documented after corneal or intraocular refractive surgery, in normal healthy prepresbyopic patients. A bibliographic revision was performed; it included works published before 1st July 2017 where accommodation and/or binocularity was assessed following any type of refractive surgical procedure. The search in Pubmed yielded 1273 papers, 95 of which fulfilled the inclusion criteria. Few publications reporting binocular vision and/or accommodative changes after refractive surgery in normal subjects were found. The reduction in fusional vergence is the most frequently reported alteration. Anisometropia is an important risk factor for postoperativ…

Examples of anthropogenic sinkholes in Sicily and comparison with similar phenomena in southern Italy

2013

A sinkhole, occurred in June 2011 and related to an underground quarry in the eastern sector of Marsala, is described in this paper as a case study (Figure 2). The site was selected for the availability of topographic data of the underground quarry, prior to the formation of the Abstract Anthropogenic sinkholes affect several built-up areas of Sicily (southern Italy) representing a great risk to people, buildings, and infrastructures. These phenomena are generally associated with the presence of ancient underground quarries for the extraction of calcarenite rock, used for building or ornamental materials. These quarries were poorly constructed and abandoned throughout history.

An automatic approach for rill network extraction to measure rill erosion by terrestrial and low‐cost unmanned aerial vehicle photogrammetry

2019

For an erosion event (October 2016) occurred at the Sparacia experimental area (Southern Italy), both terrestrial and low-altitude aerial surveys were carried out by consumer grade camera and quadcopter (low-cost unmanned aerial vehicle [UAV]) to measure rill erosion on two plots with steepness of 22% and 26%. Applying the structure from motion (SfM) technique, the three-dimensional digital terrain models (3D-DTMs) and the quasi three-dimensional models (2.5D-digital elevation model [DEM]) were obtained by the two surveys. Furthermore, 3D-DTM and DEM were built using the available aerial photographs (166) and adding 40 terrestrial photographs. For the first time, the convergence index was a…