Search results for "FAST"

An automatic multidimensional chromatography system for purification of human uterine progesterone receptor and induction of polyclonal antibodies.

1986

Abstract This paper reports on the synthesis of Org2058-bonded microparticulate silicas and their use in affinity chromatography as the first step for the purification of human progesterone receptor. The development of microprocessor-controlled instruments allows all the various steps to be performed automatically. The various steps used for the purification of human progesterone receptor were carried out with the FPLC system: (1) affinity chromatography, (2) desalting of eluate on Sephadex G-25, (3) anion-exchange chromatography using a Mono Q column. With this procedure the receptor was purified approx. 10,000-fold within 24 h. The yield of receptor was generally 85–95%. Investigations wi…

Methods for the rapid detection, isolation and sequence determination of “peptaibols” and other aib-containing peptides of fungal origin. I. Gliodeli…

1984

A rapid and simple detection procedure of Aib-containing “peptaibol” polypeptide antibiotics (mycotoxins) in fungal culture broths is described which employs alkylsilica (Sep-Pak®) cartridges for a selective enrichment, and the utilization of the unusualα-aminoisobutyric acid (Aib) and amino alcohols as specific marker constituents, which are easily detectable in total hydrolysates. Preparative isolations from fermentation broths is facilitated by adsorber resin (XAD) column chromatography, and purification of crude isolates is achieved by silica gel- and sephadex LH-20 chromatography. Preparative HPLC using spherical, totally porous ODS-bonded phases enables the isolation of uniform peptid…

Staining mitochondria in Saccharomyces cerevisiae.

1969

After testing various procedures (amidoblack 10B, acid fuchsin-methyl blue, Luxol fast blue MBS-phloxine, toluidine blue O, Jams green B and pinacyanol), three stains can be recommended for staining both types of mitochondria (globose and threadlike) in the cells of Saccharomyces cerevisiae: (1) 0.1% solution of amidoblack 10B in citrate buffer (pH 3.0) for 10 min; (2) 0.01% solution of toluidine blue O in phosphate buffer (pH 6.0) for 30 min; (3) 0.01% solution of Janus green B in distilled water (pH 5.6) for 30 min. The latter stain is most specific because its staining reaction depends upon the action of the mitochondrial enzyme cytochrome c oxidase. Yet, low concentrations and short inc…

Rapid detergent exchange in solutions of the membrane protein bacteriorhodopsin by preparative high-performance liquid chromatography (HPLC)

1984

Regulation of type IV collagen gene expression and degradation in fast and slow muscles during dexamethasone treatment and exercise.

2003

Glucocorticoids have anti-anabolic effects on many tissues and can cause muscle atrophy. However, their effects on type IV collagen gene expression and degradation in skeletal muscle have not been studied previously. Rats were treated daily with dexamethasone or saline. Half the groups of experimental and control animals were also subjected to daily endurance or uphill running exercise to determine the possible preventive effects of exercise. After an experimental period of 3 or 10 days, the extensor digitorum longus, soleus and tibialis anterior muscles were studied. Dexamethasone treatment for 10 days reduced muscle weight and type IV collagen mRNA abundance in all muscles. Gene expressio…

Studies in organic mass spectrometry. Part 17—Formation of phenol radical ions by rearrangement of the molecular ions of someN-arylthiophenecarboxami…

1995

It has been shown by exact mass measurements and collision-induced dissociation mass-analysed ion kinetic energy spectra that the structure of the m/z 124 ion observed in the mass spectra of N-(4-methoxyphenyl)thiophene-2-carboxamide, N-(4-methoxyphenyl)thiophene-3-carboxamide, N-(4-methoxyphenyl)-5-nitrothiophene-3-carboxamide and N-(4-methoxyphenyl)benzamide is identical with that of the molecular ion of 4-methoxyphenol. This ion becomes abundant in metastable energy window reactions. A probable mechanism for its formation is discussed.

Antimonic acid hydrate xerogels as proton electrolytes

1993

Abstract Two high stability types of protonic solid electrolytes based on antimonic acid hydrate xerogels have been obtained by the sol-gel technique: Sb 2 O 5 ·(3–4)H 2 O (colloidal) and Sb 2 O 5 ·5H 2 O (polymeric). The first one is a white compact material with conductivity 0.40 mS/cm (298 K) for n =3.7. The last one is transparent for visible light with a conductivity of 0.80 mS/cm. An electrochromic system based on the colloidal gel electrolyte in the form of paste has good performance-more than 10 7 cycles and a lifetime of more than five years.

Pattern Matching and Pattern Discovery Algorithms for Protein Topologies

2001

We describe algorithms for pattern-matching and pattern-learning in TOPS diagrams (formal descriptions of protein topologies). These problems can be reduced to checking for subgraph isomorphism and finding maximal common subgraphs in a restricted class of ordered graphs. We have developed a subgraph isomorphism algorithm for ordered graphs, which performs well on the given set of data. The maximal common subgraph problem then is solved by repeated subgraph extension and checking for isomorphisms. Despite its apparent inefficiency, this approach yields an algorithm with time complexity proportional to the number of graphs in the input set and is still practical on the given set of data. As a…

A Fiber Optic Strain Gage Sensor for Measuring Preload in Thick Composite Bolted Joints

2019

Mechanical fastening is a popular choice in joining composites because of the ability to transfer high loads and the ease of assembly and disassembly. In this study, the failure behavior of composite–aluminum single lap bolted joint is investigated. In particular, the effects of varying the preload on the bolt are examined. In order to accurately measure the preload, a specialized sensor that uses a fiber Bragg grating sensor embedded in the bolt is proposed and created. This sensor is realized for the current tests but can be expanded to other applications. An experimental study of bolted single-lap joints varying the tightening torque value has been carried out and, in order to validate t…

Linear-size suffix tries

2016

Suffix trees are highly regarded data structures for text indexing and string algorithms [MCreight 76, Weiner 73]. For any given string w of length n = | w | , a suffix tree for w takes O ( n ) nodes and links. It is often presented as a compacted version of a suffix trie for w, where the latter is the trie (or digital search tree) built on the suffixes of w. Here the compaction process replaces each maximal chain of unary nodes with a single arc. For this, the suffix tree requires that the labels of its arcs are substrings encoded as pointers to w (or equivalent information). On the contrary, the arcs of the suffix trie are labeled by single symbols but there can be Θ ( n 2 ) nodes and lin…