Search results for "FRAM"

showing 10 items of 2142 documents

Broadening the phenotypic spectrum and physiological insights related toEIF2S3variants

2021

Mental deficiency, epilepsy, hypogonadism, microcephaly and obesity (MEHMO) syndrome is a severe X-linked syndrome caused by pathogenic variants in EIF2S3. The gene encodes the γ subunit of the eukaryotic translation initiation factor-2, eIF2, essential for protein translation. A recurrent frameshift variant is described in severely affected patients while missense variants usually cause a moderate phenotype. We identified a novel missense variant (c.433A>G, p.(Met145Val)) in EIF2S3 in a mildly affected patient. Studies on zebrafish confirm the pathogenicity of this novel variant and three previously published missense variants. CRISPR/Cas9 knockout of eif2s3 in zebrafish embryos recapitula…

MicrocephalyFrameshift mutation03 medical and health sciencesGeneticsmedicineAnimalsHumansMissense mutationGenitaliaCRISPR/Cas9GeneZebrafishZebrafishGenetics (clinical)030304 developmental biologyGenetics0303 health scienceseIF2EIF2S3biology030305 genetics & heredityapoptosisbiology.organism_classificationmedicine.diseasePhenotypePhenotypeMutationMental Retardation X-LinkedEIF2S3MEHMO syndromeHuman Mutation
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A microscope configuration for nanometer 3-D movement monitoring accuracy.

2011

In this paper we present a new microscopy configuration based upon temporal tracking of a secondary reflected speckle by imaging the speckle through properly defocused optics. The configuration is used to monitor three-dimensional (3-D) spontaneous contraction of rat cardiac muscle cells while achieving nanometer tracking accuracy at a rate of 30 frames per second (fps) without using interferometric recording. Estimation of the change in the optical path of accuracy of 50 nm in the transverse direction and of 200 nm in the axial direction was achieved.

MicroscopyMicroscopeMaterials sciencebusiness.industryFourier opticsGeneral Physics and AstronomyCell BiologyTracking (particle physics)Frame ratelaw.inventionRatsSpeckle patternInterferometryOpticsOptical pathImaging Three-DimensionalStructural BiologylawMicroscopyAnimalsGeneral Materials ScienceMyocytes CardiacbusinessCells CulturedMicron (Oxford, England : 1993)
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The impact of media and NGOs on four European Parliament discourses about conflicts in the Middle East

2017

There is empirical evidence of media influence on parliamentary agenda, especially when media coverage privileges conflict framing of reality and negativity. This article addresses the impact of me...

Middle EastSociology and Political ScienceParliamentCommunicationmedia_common.quotation_subject05 social sciencesMedia studies050801 communication & media studiesMedia coverageNegativity effect0506 political science0508 media and communicationsFraming (social sciences)Arts and Humanities (miscellaneous)Political sciencePolitical Science and International Relations050602 political science & public administrationEmpirical evidencemedia_commonMedia, War & Conflict
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The implementation of mindfulness in healthcare Systems: a theoretical analysis

2014

Abstract Objective Evidence regarding the efficacy of mindfulness-based interventions (MBIs) is increasing exponentially; however, there are still challenges to their integration in healthcare systems. Our goal is to provide a conceptual framework that addresses these challenges in order to bring about scholarly dialog and support health managers and practitioners with the implementation of MBIs in healthcare. Method This is an opinative narrative review based on theoretical and empirical data that address key issues in the implementation of mindfulness in healthcare systems, such as the training of professionals, funding and costs of interventions, cost effectiveness and innovative deliver…

MindfulnessKnowledge managementbusiness.industryCost effectivenessHealthcare systemsPsychological interventionHealth Plan ImplementationObjective EvidencePsychiatry and Mental healthConceptual frameworkNursingOrder (exchange)ImplementationStepped-care modelHealth careMedicineHumansDialog boxbusinessDelivery of Health CareMindfulnessSalut mentalPrimary Care
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CONSTRUCTIONS, FRAMES, CARTOGRAPHIES MENTALES.: ESQUISSE D'UNE MÉTHODE POUR LA REPRÉSENTATION ET LA TRANSMISSION DE CONNAISSANCES EN COURS DE LANGUES…

2020

International audience; In the last three decades the linguistic innovation has brought two main theoretical tools that are fully functional: the construction grammar and the frame semantics. The present work makes a proposal to unite both frameworks in order to exploit their respective contribution in a pedagogical perspective in foreign language classes. The aim is (i) to operationalize the theoretical junction, and (ii) to propose to exploit it by using mind maps, which elaboration is based on the observation of the speech praxis.

Mindmap[SCCO.LING] Cognitive science/LinguisticsFrame Semantic[SCCO.LING]Cognitive science/Linguistics[SHS.LANGUE]Humanities and Social Sciences/Linguistics[SHS.LANGUE] Humanities and Social Sciences/Linguistics
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The complete mitochondrial genome of Bactrocera biguttula (Bezzi) (Diptera: Tephritidae) and phylogenetic relationships with other Dacini

2018

Bactrocera biguttula is an African olive fruit fly that does not attack cultivated olives but rather develops in the fruits of wild species of Olea and Noronhia. The complete mitochondrial genome of an individual specimen was characterized in comparison to other Bactrocera. The phylogenetic relationships of B. biguttula with other Dacini were investigated, with special focus on B. oleae, an agricultural pest known to attack cultivated and wild olives. The sequence had a total length of 15,829 bp, and included the typical features of insect mitogenomes, similarly to the other Bactrocera analysed. Start codons included ATG, ATC, ATT, and TCG (in COI). The majority of stop codons (TAA) were fu…

Mitochondrial DNAOlive fruit fly02 engineering and technologyBiochemistryOpen Reading Frames03 medical and health sciencesComplete sequenceRNA TransferStructural BiologyPhylogeneticsTephritidaeAnimalsBactroceraCodonMolecular BiologyPhylogeny030304 developmental biologyBase CompositionLikelihood Functions0303 health sciencesPhylogenetic treebiologyTephritidaeBayes TheoremGeneral MedicineAfrican olive fruit fly021001 nanoscience & nanotechnologybiology.organism_classificationStop codonMitogenomeWild olivesEvolutionary biologyGenome MitochondrialNucleic Acid ConformationDNA Circular0210 nano-technologyInternational Journal of Biological Macromolecules
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Bioinspired Metal-Organic Frameworks in Mixed Matrix Membranes for Efficient Static/Dynamic Removal of Mercury from Water

2020

The mercury removal efficiency of a novel metal-organic framework (MOF) derived from the amino acid S-methyl-L-cysteine is presented and the process is characterized by single-crystal X-ray crystallography. A feasibility study is further presented on the performance of this MOF and also that of another MOF derived from the amino acid L-methionine when used as the sorbent in mixed matrix membranes (MMMs). These MOF-based MMMs exhibit high efficiency and selectivity in both static and dynamic regimes in the removal of Hg2+ from aqueous environments, due to the high density of thioalkyl groups decorating MOF channels. Both MMMs are capable to reduce different concentration of the pollutant to …

Mixed matrixMaterials scienceGroundwater remediationchemistry.chemical_element02 engineering and technology010402 general chemistryAigua potable Depuració01 natural sciencesBiomaterialscapture devicemercury(II)ElectrochemistryMaterialsmetal-organic frameworksfungiwater remediation021001 nanoscience & nanotechnologyCondensed Matter Physics6. Clean water0104 chemical sciencesElectronic Optical and Magnetic MaterialsMercury (element)MembranechemistryChemical engineeringMetal-organic frameworkmixed matrix membranes0210 nano-technology
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Molecular evolutionary analysis of type-1 human astroviruses identifies putative sites under selection pressure on the capsid protein

2017

Human astroviruses (HAstV) are important enteric pathogens that can be classified into eight sero/genotypes (HAstV-1 to -8). Although the various HAstV types show global spread, type-1 strains tend to be predominant. Molecular analysis of the genomic region encoding the capsid protein (ORF2) has revealed discrete sequence variation, with different lineages within each HAstV type and at least three major lineages have been identified within HAstV-1. Longitudinal epidemiological surveillance has revealed temporal shift of the various HAstV-1 lineages. Metadata analysis of HAstV-1 sequences available in the databases also revealed temporal shifts of the circulation of HAstV-1 lineages, suggest…

Models Molecular0301 basic medicineMicrobiology (medical)Settore MED/07 - Microbiologia E Microbiologia ClinicaGenotypeProtein ConformationAstroviru030106 microbiologyBiologyMicrobiologyAstrovirusEvolution MolecularOpen Reading Frames03 medical and health sciencesProtein structureGeneticPhylogeneticsAstroviridae InfectionsGenetic variationGenotypePhylogenetic analyseGeneticsHumansAmino Acid SequenceSelection GeneticPeptide sequenceMolecular BiologyPhylogenyEcology Evolution Behavior and SystematicsGeneticsGenetic evolutionSelection pressure analysiGenetic Variationbiology.organism_classificationEcology Evolution Behavior and SystematicOpen reading frame030104 developmental biologyInfectious DiseasesCapsidAstroviridaeCapsid ProteinsCapsid protein structureHAstV-1
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Correction to ‘Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy’

2021

The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5' end, the ribosomal frameshift segment and the 3'-un…

Models Molecular2019-20 coronavirus outbreakMagnetic Resonance SpectroscopyCoronavirus disease 2019 (COVID-19)AcademicSubjects/SCI00010Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Genome ViralBiology03 medical and health sciencesGeneticsHumans3' Untranslated RegionsPandemicsProtein secondary structure030304 developmental biology0303 health sciencesBase SequenceSARS-CoV-2030302 biochemistry & molecular biologyCOVID-19Frameshifting RibosomalRNANuclear magnetic resonance spectroscopyVirologyNucleic Acid ConformationRNA ViralCorrigendumNucleic Acids Research
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A Biocompatible Aspartic-Decorated Metal–Organic Framework with Tubular Motif Degradable under Physiological Conditions

2021

Achieving a precise control of the final structure of metal–organic frameworks (MOFs) is necessary to obtain desired physical properties. Here, we describe how the use of a metalloligand design strategy and a judicious choice of ligands inspired from nature is a versatile approach to succeed in this challenging task. We report a new porous chiral MOF, with the formula Ca5II{CuII10[(S,S)-aspartamox]5}·160H2O (1), constructed from Cu2+ and Ca2+ ions and aspartic acid-decorated ligands, where biometal Cu2+ ions are bridged by the carboxylate groups of aspartic acid moieties. The structure of MOF 1 reveals an infinite network of basket-like cages, built by 10 crystallographically distinct Cu(II…

Models MolecularBiocompatibilityMetal ions in aqueous solutionBiocompatible Materials010402 general chemistry01 natural sciencesArticleInorganic Chemistrychemistry.chemical_compoundAdsorptionAspartic acidTumor Cells CulturedHumansCarboxylatePhysical and Theoretical ChemistryMetal-Organic Frameworkschemistry.chemical_classificationAspartic AcidMolecular Structure010405 organic chemistryCombinatorial chemistry0104 chemical sciencesAmino acidchemistryMetal-organic frameworkDrug carrierInorganic Chemistry
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