Search results for "FRAM"
showing 10 items of 2142 documents
Partial vinylphenol reductase purification and characterization from Brettanomyces bruxellensis
2008
International audience; Brettanomyces is the major microbial cause for wine spoilage worldwide and causes significant economic losses. The reasons are the production of ethylphenols that lead to an unpleasant taint described as 'phenolic odour'. Despite its economic importance, Brettanomyces has remained poorly studied at the metabolic level. The origin of the ethylphenol results from the conversion of vinylphenols in ethylphenol by Brettanomyces hydroxycinnamate decarboxylase. However, no information is available on the vinylphenol reductase responsible for the conversion of vinylphenols in ethylphenols. In this study, a vinylphenol reductase was partially purified from Brettanomyces bruxe…
Homochiral Metal-Organic Frameworks for Enantioselective Separations in Liquid Chromatography
2020
Selective separation of enantiomers is a substantial challenge for the pharmaceutical industry. Chromatography on chiral stationary phases is the standard method, but at a very high cost for industrial-scale purification due to the high cost of the chiral stationary phases. Typically, these materials are poorly robust, expensive to manufacture, and often too specific for a single desired substrate, lacking desirable versatility across different chiral analytes. Here, we disclose a porous, robust homochiral metal–organic framework (MOF), TAMOF-1, built from copper(II) and an affordable linker prepared from natural l-histidine. TAMOF-1 has shown to be able to separate a variety of model…
Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracel…
2010
Abstract Background A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics. Results One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resis…
Fostering Collaborative Governance in Chronic Disease Management Programs: A Dynamic Performance Management Approach
2020
Chronic diseases are the leading cause of disability and mortality in the world and represent a global health emergency due to the increase in frequency and complexity that has been occurring in recent years. The outcomes related to chronic care needs depend on the joint effort of a multi-provider, multi-disciplinary, and multi-professional service network, which operates along a clinical pathway. However, all the different players involved in the provision of services may have different interests and goals derived from their organizational structure and their role in the overall health system. This context of fragmented governance makes performance management of care services problematic. …
Towards Next Generation System Architecture for Emergency Services
2011
European Union has decided that all emergencies can be reported to authorities by European citizens by calling 112 or sending a text message to 112. Distributing warnings and alerts of authorities to citizens currently happens through national TV and radio channels, but telecom networks are also used now in some countries for this purpose. During the last ten years there have been attempts to develop new system architectures for various emergency service provision phases. Some of them are already deployed in special cases, like in ambulance services. Multimedia emergency alert messages can be delivered to citizens over fixed and mobile telecom networks and commercial systems are already in …
Global trends in a fragile context : public–nonpublic collaboration, service delivery and social innovation
2020
PurposeThis study aims to enhance the understanding of the nature of collaboration between public and nonpublic actors in delivering social services and achieving social innovation in a fragile context, with an emphasis on the role of civil society organisations (CSOs). The paper focuses on Bosnia and Herzegovina, a Southeastern European country which has faced a turbulent post-conflict transition and experienced challenges in its social welfare policy and practice.Design/methodology/approachThis study uses institutional theory, particularly new institutionalism and institutional networking, as a lens through which to understand public and nonpublic collaboration and social innovation withi…
Frames for fusions of modal logics
2018
Let us consider multimodal logics and . We assume that is characterised by a class of connected frames, and there exists an -frame with a so-called -starting point. Similarly, the logic is characterised by a class of connected frames, and there exists an -frame with a -starting point. Using isomorphic copies of the frames and , we construct a connected frame which characterises the fusion . The frame thus obtained has some useful properties. Among others, is countable if both and are countable, and there is a special world of the frame such that any formula is valid in the frame if and only if it is valid at the point . We also describe a similar construction where we assume the existence o…
Stability of genetic regulatory networks with time-varying delay: Delta operator method
2015
This paper investigates the stability problem for a class of uncertain genetic regulatory networks (GRNs) with time-varying delay via delta operator approach. Both the parameter uncertainty and the generalized activations are considered in the model under study. By constructing an appropriate Lyapunov-Krasovskii functional, the stability and robust stability conditions of GRNs are presented under the delta operator frame. These conditions can be expressed in terms of linear matrix inequalities (LMIs). Finally, a numerical example is employed to illustrate the effectiveness of the proposed results.
Reframing Climate Justice : A Three-dimensional View on Just Climate Negotiations
2016
This article proposes reframing the justice discourse in climate negotiations. In so doing, it makes two claims. First, global climate negotiations deserve to be addressed as an issue of justice on their own due to their peculiar characteristics. Second, a multidimensional theory of justice is superior to distributional theories for this task. To support these arguments, I apply the multidimensional theory of justice to global climate negotiations. This analysis reveals that injustice in the negotiations is multidimensional and irreducible to distributional questions. Furthermore, it shows how promoting justice in this broad sense would have significant effect on the negotiation procedures …
Sequencing and analysis of the gene encoding the α-toxin of Clostridium novyi proves its homology to toxins A and B of Clostridium difficile
1995
A library of total Clostridium novyi DNA was established and screened for the alpha-toxin gene (tcn alpha) by hybridization with oligonucleotides derived from a partial N-terminal sequence and by using specific antisera. Overlapping subgenic tcn alpha fragments were isolated and subsequently the total sequence of tcn alpha was determined. The 6534 nucleotide open reading frame encodes a polypeptide of M(r) 250,166 and pI 5.9. The N-terminal alpha-toxin (Tcn alpha) sequence MLITREQLMKIASIP determined by Edman degradation confirmed the identity of the reading frame and the assignment of the translation start point. The toxin is not modified posttranslationally at its N-terminus nor does it co…