Search results for "Fermentation"
showing 10 items of 746 documents
Kinetics of solid-state fermentation of lupin with Rhizophus oligosporus based on nitrogen compounds balance
2021
Solid-state fermentation could be a potentially effective method for improving the nutritional value of legumes. The objective of this study was to explore this technology in Lupinus mutabilis, a species of lupin, using a strain of Rhizopus oligosporus mold for solid-state fermentation. Three L. mutabilis varieties (INIAP-450, INIAP-451, and Criollo) were evaluated, including the effect of the grain status (whole or ground) and integument or husk (presence or absence). Kinetic parameters of the fermentation process were studied to define the specific speed of nitrogen formation and the digestibility and nutritional quality of the resulting protein. After incubation at 28 °C for 96 h, the ti…
Optimization of alkali pretreatment to enhance rice straw conversion to butanol
2021
Abstract The use of rice straw (RS) was enhanced to produce biobutanol as biofuel, for which the NaOH pretreatment was optimized by considering the butanol-biomass ratio that quantify the mass balance efficiency of the three sequential stages of the process: pretreatment, enzymatic hydrolysis and fermentation by Clostridium beijerinckii. The optimum point (solid loading of 5% w/v with 0.75% w/v NaOH at 134 °C for 20 min) of the best cost-wise option yielded an enhanced biomass use of 77.6 g kg RS−1. A maximum butanol titer of 10.1 g L−1 was reached after 72 h of fermentation with the complete uptake of glucose and nearly complete uptake of xylose. The NaOH concentration was the most influen…
Thermophilic hydrogen production from cellulose with rumen fluid enrichment cultures: Effects of different heat treatments
2011
Elevated temperatures (52, 60 and 65 °C) were used to enrich hydrogen producers on cellulose from cow rumen fluid. Methanogens were inhibited with two different heat treatments. Hydrogen production was considerable at 60 °C with the highest H2 yield of 0.44 mol-H2 mol-hexose -1 (1.93 mol-H2 mol-hexose-degraded-1) as obtained without heat treatment and with acetate and ethanol as the main fermentation products. H2 production rates and yields were controlled by cellulose degradation that was at the highest 21%. The optimum temperature and pH for H2 production of the rumen fluid enrichment culture were 62 °C and 7.3, respectively. The enrichments at 52 and 60 °C contained mainly bacteria from …
Acetaldehyd als Indicator f�r die Regulation von Atmung und G�rung bei der aeroben Verg�rung von Glucose durch Saccharomyces cerevisiae
1971
Wahrend der aeroben Vergarung von Glucose wurde die Konzentration von Acetaldehyd im Garmedium uber den gesamten Garablauf bei mehreren Stammen von Saccharomyces cerevisiae verfolgt. Die Aldehydkonzentration weist bei Glucosekonzentrationen zwischen 5 und 20% zwei Maxima auf. Damit ist der Konzentrationsverlauf von Acetaldehyd aerob wesentlich anders als bei der anaeroben Garung, mit nur einem meist niedrigen Maximum. 10-3 M Azid hemmt die Bildung von Acetaldehyd ganz oder weitgehend. Das deutet auf die Funktion bzw. Synthese der Cytochrome, die in Gegenwart von Sauerstoff offensichtlich auch bei hohen Glucosekonzentrationen nicht vollstandig reprimiert werden. Der durch die Atmung bedingte…
Formation of l(-)malate by Saccharomyces cerevisiae during fermentation
1988
When grown in a synthetic medium most of the 51 strains of the genera Saccharomyces, Saccharomycodes, Zygosaccharomyces and Schizosaccharomyces investigated formed l-malate during fermentation. The quantity varied between 0.1 and 2.6 g malate per liter. Two strains of Saccharomyces cerevisiae synthesized malate at a rate of about 1.5 g/l. Malate was liberated during the growth phase and not metabolized during the stationary phase. Optimum malate formation was observed at a sugar concentration of about 20% (w/v), at pH 5 and at suboptimal nitrogen concentrations of less than 300 mg N/liter. Of the amino acids aspartate and glutamate were most favourable. If ammonium salts were used as the ni…
Enhanced fermentative capacity of yeasts engineered in storage carbohydrate metabolism.
2014
During yeast biomass production, cells are grown through several batch and fed-batch cultures on molasses. This industrial process produces several types of stresses along the process, including thermic, osmotic, starvation, and oxidative stress. It has been shown that Saccharomyces cerevisiae strains with enhanced stress resistance present enhanced fermentative capacity of yeast biomass produced. On the other hand, storage carbohydrates have been related to several types of stress resistance in S. cerevisiae. Here we have engineered industrial strains in storage carbohydrate metabolism by overexpressing the GSY2 gene, that encodes the glycogen synthase enzyme, and deleting NTH1 gene, that …
Protection of living yeast cells by micro-organized shells of natural polyelectrolytes
2015
International audience; Saccharomyces cerevisiae, a eukaryotic model organism, plays a key role in the oxidative stability of fermented products. In order to protect cells against environmental stresses, we report a method of modifying the cell surface architecture while maintaining the internal working properties of the system. The objective was to encapsulate living yeast cells in micro-organized polyelectrolyte shells using layer-by-layer (LbL) assembly. For the first time, the natural polyelectrolytes, β-lactoglobulin and sodium alginate, were alternately deposited on the surface of S. cerevisiae. Transmission electron microscopy coupled with immune-cytochemistry and scanning electron m…
Application of MALDI-TOF-MS and nested SAPD-PCR for discrimination ofOenococcus oeniisolates at the strain level
2015
Oenococcus oeni is the most important lactic acid bacterium during vinification and is frequently used as a commercial starter culture for malolactic fermentation. Since different strains exhibit a high heterogeneity concerning wine-related physiological characteristics, reliable methods for their differentiation at the strain level are needed, especially for the development of starter cultures. Microbial identification by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has increased over the last few years. In the present study, we used MALDI-TOF-MS for the differentiation of 11 O. oeni isolates originating from 5 different wine-growing reg…
On the fermentative behavior of auxotrophic strains of Saccharomyces cerevisiae
2014
Background: The selection of new yeast strains could lead to improvements in bioethanol production. Here, we have studied the fermentative capacity of different auxotrophic mutants of Saccharomyces cerevisiae, which are routinely used as hosts for the production of heterologous proteins. It has recently been found that these strains exhibit physiological alterations and peculiar sensitivities with respect to the parental prototrophic strains from which they derive. In this work the performance of auxotrophic S. cerevisiae CEN.PK strains was compared to the corresponding prototrophic strain, to S. cerevisiae T5bV, a strain isolated from grape must and to another auxotrophic strain, S. cerevi…
Expanding the chemical diversity of an endophytic fungus Bulgaria inquinans, an ascomycete associated with mistletoe, through an OSMAC approach
2019
An endophytic fungus Bulgaria inquinans (isolate MSp3-1), isolated from mistletoe (Viscum album), was subjected to fermentation on solid Czapek medium. Chromatographic workup of the crude EtOAc extract yielded five new natural products (1–5). Subsequent application of the “One Strain, MAny Compounds” (OSMAC) strategy on this strain by the addition of a mixture of salts (MgSO4, NaNO3 and NaCl) to solid Czapek medium induced the accumulation of nine additional new secondary metabolites (6–13, 16), with most of them (8, 10–12) not detectable in cultures lacking the salt mixture. The structures of the new compounds were established on the basis of the 1D/2D NMR and HRESIMS data. The TDDFT-ECD m…