Search results for "Ficoll"

Siboc, Chotacabras pardo (VER0000118)

Altres noms vulgars: Red-necked Nightjar (Anglès), Engoulevent à collier roux (Francès), Rothals-Ziegenmelker (Alemany) Gabinet de Vertebrats (Departament de Zoologia), Facultat de Ciències Biològiques (Campus de Burjassot), C/ Doctor Moliner, s/n, Bloque B. 5é plant, Burjassot (Valencia). Armari: 31-5 Valencia

An old method for good new cells

2009

The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4 degrees C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination (P < .001 by chi-square).

Falcó selvàtic barrat, Halcón-selvático barrado (VER0000148)

Altres noms vulgars: Barred forest-falcon (Anglès), Carnifex barré (Francès), Sperberwaldfalke (Alemany) Gabinet de Vertebrats (Departament de Zoologia), Facultat de Ciències Biològiques (Campus de Burjassot), C/ Doctor Moliner, s/n, Bloque B. 5é plant, Burjassot (Valencia). Armari: 4-1 América Central Macho Adulto

Falcó selvàtic barrat, Halcón-selvático barrado (VER0000149)

1858

Altres noms vulgars: Barred forest-falcon (Anglès), Carnifex barré (Francès), Sperberwaldfalke (Alemany) Gabinet de Vertebrats (Departament de Zoologia), Facultat de Ciències Biològiques (Campus de Burjassot), C/ Doctor Moliner, s/n, Bloque B. 5é plant, Burjassot (Valencia). Armari: 4-1 Nueva Granada 00/04/1858 Hembra Adulto

Attachment of Marine Sponge Cells of Hymeniacidon perleve on Microcarriers

2003

Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were studied. Mixed cell population and enriched cell fractions of specific cell types by Ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. Cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on Cytodex 3 and Hillex for both mixed cell population and cell fraction at Ficoll 15-20% interface. The h…