Search results for "Firmicutes"
showing 9 items of 59 documents
I batteri associati al nettare fiorale influiscono sulle longevità degli insetti parassitoidi
2023
I parassitoidi adulti frequentemente visitano le piante in fioritura, ricercando nettare fiorale per coprire i loro bisogni energetici e nutrizionali. In letteratura, infatti, i benefici del nettare sulla fitness dei parassitoidi sono ampiamente documentati. Sebbene il nettare fiorale costituisca un substrato ideale per la crescita di comunità microbiche che possono alterarne le caratteristiche organolettiche, l'effetto della fermentazione batterica del nettare sui parassitoidi non è stato ancora sufficientemente esplorato. In questo lavoro è stata studiata la longevità dei parassitoidi adulti utilizzando nettare sintetico fermentato da diversi ceppi batterici appartenenti ai phyla Firmicut…
Culturable endophytic microbial communities in the circumpolar grass,Deschampsia flexuosain a sub-Arctic inland primary succession are habitat and gr…
2014
Summary Little is known about endophytic microbes in cold climate plants and how their communities are formed. We compared culturable putative endophytic bacteria and fungi in the ecologically important circumpolar grass, Deschampsia flexuosa growing in two successional stages of subarctic sand dune (68°29′N). Sequence analyses of partial 16S rRNA and internal transcribed spacer (ITS) sequences of culturable endophytes showed that diverse bacteria and fungi inhabit different tissues of D. flexuosa. A total of 178 bacterial isolates representing seven taxonomic divisions, Alpha, Beta and Gammaproteobacteria, Actinobacteria, Bacteroidetes, Firmicutes and Acidobacteria, and 30 fungal isolates …
Differential responses of bacterial and archaeal groups at high taxonomical ranks to soil management
2010
Little is known about abundances of the major bacterial taxa in agricultural soils and how they are affected by fertilization or other agricultural practices. Our aim was to determine the abundance and relative distribution of several bacterial phyla and one class, as well as the archaeal and crenarchaeal communities, and how they were affected by different fertilization regimes to examine whether specific responses of microorganisms could be identified at these high taxonomic ranks. We used real-time PCR with taxa specific primers to quantify the abundance of the Actinobacteria, Acidobacteria, Bacteriodetes, Firmicutes, Gemmatimonadetes, Verrucomicrobia, Alphaproteobacteria and Crenarchaeo…
Aerobic Endospore-forming Bacteria and Soil Invertebrates
2011
The intestinal microbiotas of only a few soil invertebrates such as collembola (springtails), earthworms, nematodes, isopods (woodlice and pill bugs), millipedes and termites have been studied by classical and molecular methods in the last decades. It became obvious that these lower members of the soil biota harbour complex microbial communities that sometimes reach counts of 1011 cells ml−1 in their intestinal tracts. The gut microbiotas of the soil fauna include a variety of microorganisms from all three domains of life (Bacteria, Archaea, Eucarya). The bacterial species can mainly be assigned to the Gram-positive phyla Firmicutes and Actinobacteria as well as the Gram-negative phyla Prot…
Gut Microbiome Analysis In Adult Tropical Gars (Atractosteus tropicus)
2019
AbstractTropical gar (Atractosteus tropicus), is freshwater and estuarine fish that has inhabited the Earth since the Mesozoic era, undergoing limited physiological variation ever since. This omnivorous fish is endemic to southern Mexico and part of Central America. Besides its recognized cultural and scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. Previous studies have highlighted the role of microbial communities in fish, particularly those in the gut microbiome, in maintaining their host homeostasis or disease. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents of …
Warm and wet: robust lipase-producing bacteria from the indoor environment
2017
Lipases are key biocatalysts with important biotechnological applications. With the aim of isolating robust lipolytic microbial strains, we have analyzed the bacterial communities inhabiting two domestic extreme environments: a thermophilic sauna and a dishwasher filter. Scanning electron microscopy revealed biofilm-forming and scattered microorganisms in the sauna and dishwasher sample, respectively. A culture-independent approach based on 16S rRNA analysis indicated a high abundance of Proteobacteria in the sauna sample; and, a large amount of Proteobacteria, Firmicutes, Cyanobacteria and Actinobacteria in the dishwasher filter. With a culture-dependent approach, we isolated 48 bacterial …
Metagenomic Analysis of Milk of Healthy and Mastitis-Suffering Women.
2015
Background: Some studies have been conducted to assess the composition of the bacterial communities inhabiting human milk, but they did not evaluate the presence of other microorganisms, such as fungi, archaea, protozoa, or viruses. Objective: This study aimed to compare the metagenome of human milk samples provided by healthy and mastitis-suffering women. Methods: DNA was isolated from human milk samples collected from 10 healthy women and 10 women with symptoms of lactational mastitis. Shotgun libraries from total extracted DNA were constructed and the libraries were sequenced by 454 pyrosequencing. Results: The amount of human DNA sequences was ≥ 90% in all the samples. Among the bacteri…
Physical Activity Differentially Affects the Cecal Microbiota of Ovariectomized Female Rats Selectively Bred for High and Low Aerobic Capacity
2015
The gut microbiota is considered a relevant factor in obesity and associated metabolic diseases, for which postmenopausal women are particularly at risk. Increasing physical activity has been recognized as an efficacious approach to prevent or treat obesity, yet the impact of physical activity on the microbiota remains under-investigated. We examined the impacts of voluntary exercise on host metabolism and gut microbiota in ovariectomized (OVX) high capacity (HCR) and low capacity running (LCR) rats. HCR and LCR rats (age = 27 wk) were OVX and fed a high-fat diet (45% kcal fat) ad libitum and housed in cages equipped with (exercise, EX) or without (sedentary, SED) running wheels for 11 wk (…
Saldūdens dūņu mikroorganismu populāciju dinamika jēlglicerīna fermentācijas laikā
2019
Anaerobās fermentācijas ceļā var lietderīgi izmantot jēlglicerīnu, biodīzeļa ražošanas blakusproduktu, iegūstot produktus ar pievienotu vērtību, kas ļauj padarīt biodīzeļa ražošanu ekonomiski izdevīgāku. Šajā darbā tika pētīta mikroorganismu populāciju dinamika jēlglicerīna anaerobās fermentācijas laikā, izmantojot saldūdens dūņas kā inokulātu. Fermentāciju veica 20°C un 37°C temperatūrā 4 un 8 dienas. Noteica izdalītās gāzes tilpumu, kā arī barotņu organisko vielu daudzumu un sastāvu, nosakot ķīmiskā skābekļa patēriņu un veicot šķidruma hromatogrāfiju. Pētīja mikroorganismu populāciju dinamiku, veicot qPCR ar dažādiem baktēriju taksoniem specifiskiem 16S rDNS praimeru pāriem, kā arī veicot…