Search results for "Flask"

Seju un logo aptazīšanas tīmekļa sistēma

2017

Šajā dokumentā ir aprakstīta seju un logo atpazīšanas tīmekļa sistēma, kas paredzēta attēlos attēlotu seju atrašanai un no šīm sejām sistēmāievietoto seju atpazīšanai, kā arī attēlos redzamo logotipu, kas ievietoti sistēmā, atpazīšanai. Sistēmai tika aprakstīta veidota tikai aizmugures daļa, jo lietotāju saskarni implementēs pasūtītājs. Sistēma realizēta, izmantojot ārējus moduļus, Docker platformā. Tā implementēta Python valodā, izmantojot Flask ietvaru.

Influence of the concentration of oxygen on the swimming path of Brachionus plicatilis (Rotifera)

1993

Brachionus plicatilis females were maintained for > 24 hours in water where the concentration of oxygen was precisely controlled (spherical flasks with ≥ 6 mg 1−1 or < 0.5 mg 1−1; food = dead Tetraselmis sp.). Each female was randomly taken from one flask and quickly placed in an observation chamber containing the same experimental conditions. The swimming path was videotaped (5 minutes); then the size of the female was measured. The tape was analyzed by automatic tracking (25 x, y coordinates of the center of the animal, in a 512 × 512 pixels space). The swimming path was analyzed for ~45 females in both treatments. The speed (mm s−1 body length−1) was calculated for all trajectories, or o…

FACTORS AFFECTING CHINESE HAMSTER OVARY CELL PROLIFERATION AND VIABILITY

2019

Advantageous cultivation procedures for the Chinese hamster ovary (CHO) cells are necessary for the productive commercial production of biopharmaceuticals. A main challenge that needs to be addressed during the process development is the differences in each cell line requirements concerning the nutrients and feed strategies in order to achieve the desired growth characteristics. Therefore, within the current research, a naïve high cell density serum free suspension adapted CHO cell line was tested with glucose and glutamine rich feeds in fed-batch Erlenmeyer shake flask cultures. Glucose consumption rate was adjusted to develop the optimal feed strategies. Obtained results indicated that hi…

An Optimized and Rapid DNA Extraction Method From Leaves of Grapevine Suitable for PCR-DGGE Based Analysis

2010

Molecular techniques and genetic studies require a fair amount of DNA of high quality in order to produce reliable and clear results (Li et al., 2007). In grapevine, the yield and quality of DNA can be significantly affected by secondary metabolites such as polyphenols, polysaccharides and tannins, that may be consistently abundant during the different stages of leaf development (Iandolino et al., 2004). The development of a simple, rapid and reliable method for the extraction of genomic DNA from grape leaves collected at several stages during development, as well as from healthy grape leaves and leaves infected by pathogenic microorganisms, was the main objective of this study. The protoco…

Semiautomatic growth analysis of multicellular tumor spheroids.

2011

Multicellular tumor spheroids (MCTS) are routinely employed as three-dimensional in vitro models to study tumor biology. Cultivation of MCTS in spinner flasks provides better growing conditions, especially with regard to the availability of nutrients and oxygen, when compared with microtiter plates. The main endpoint of drug response experiments is spheroid size. It is common practice to analyze spheroid size manually with a microscope and an ocular micrometer. This requires removal of some spheroids from the flask, which entails major limitations such as loss of MCTS and the risk of contamination. With this new approach, the authors present an efficient and highly reproducible method to an…

Microelectrode Measurements of Oxygen Tension Distributions in Multicellular Spheroids Cultured in Spinner Flasks

1984

Since multicellular spheroids were introduced into cancer research by Sutherland et al. (1970, 1971), they have been widely used for studying the susceptibility of cancer cells to various treatment modalities. Many of these investigations provided evidence for a major role of oxygen in controlling the metabolic and cell cycle state of the tumor cells, thus modifying the responsiveness of the cells to therapy. Several investigators have measured O2 tension (PO2) distributions in spheroids with O2-sensitive microelectrodes (Carlsson et al. 1979; Kaufman et al. 1981; Mueller-Klieser and Sutherland 1982a, b, 1983; Mueller-Klieser et al. 1983). The measurements produced PO2 values that varied co…

Rinnakkaislaskenta osittaisdifferentiaaliyhtälöiden numeerisessa ratkaisemisessa

1984

Skrējiena datu analīze

2022

Kvalifikācijas darba “Skrējiena datu analīze” ietvaros ir izstrādāta sistēma tīmekļa vietnes čaulā, kura sniedz iespēju lietotājam augšupielādēt skrējiena vai citas sporta aktivitātes informācijas datni un pretī saņemt viegli pārskatāmu datu apkopojumu, aktivitātes attēlojumu kartē kopā ar datu punktiem konkrētā lokācijā, datnes hierarhijas modeli interaktīvā formā, kā arī līniju un stabiņu diagrammas, kas reprezentē saņemtos datus.

A laboratory experiment on inferring Poiseuille's law for undergraduate students

2006

In this paper a laboratory experiment is proposed to infer Poiseuille's law. A simple set-up based on two flasks joined by a detachable tube allows one to measure using tubes of different radii and different lengths. One of the flasks is connected to a vacuum pump to control the pressure differential between the tube extremes. The influence on the flow of different radii, lengths, pressures and viscosities can be studied in a didactic way by measuring the flow rate for each of these variables. The experiment can be performed getting together the students in groups, so that each group concentrates on the effect on the flow of a specific variable, leaving the rest fixed. After putting togethe…

Calibration of misonidazole labeling by simultaneous measurement of oxygen tension and labeling density in multicellular spheroids

1995

To correlate misonidazole concentrations and oxygen pressures (Po2) at identical locations within EMT6/Ro multi-cell spheroids (mean diameters +/- SD: 867 +/- 20 microns), Po2 measurements were performed with oxygen-sensitive microelectrodes during incubation of these spheroids with tritiated misonidazole (10 mg/I; 445 microCi/mg). In each individual spheroid, Po2 profiles were correlated with the corresponding spatial distribution of misonidazole as quantified by conventional autoradiography and grain counting. To compare the oxygenation status of spheroids in the measuring chamber with that of spheroids in spinner culture, misonidazole labeling was performed in both environments following…