Search results for "Fluorescence"

Localization of antigens PwA33 and La on lampbrush chromosomes and on nucleoplasmic structures in the oocyte of the urodele Pleurodeles waltl: Light …

1994

Monoclonal antibodies A33/22 and La11G7 have been used to study the distribution of the corre-sponding antigens, PwA33 and La, on the lampbrush chromosome loops and nucleoplasmic structures of P. waltl oocytes, using immunofluorescence, confocal laser scanning microscopy and immunogold labeling. The results obtained with these antibodies have been compared with those obtained with the Sm-antigen-specific monoclonal antibody Y12. All these monoclonal antibodies (mAbs) labeled the matrices of the majority of normal loops along their whole length. Nucleoplasmic RNP granules showed a strong staining with the mAbs La11G7 and Y12 throughout their mass, but with the mAb A33/22, they showed only a …

Conformational heterogeneity of the point defects in Silica Glasses: Study of the lifetime of 2.7 eV phosphorescence band

2006

Quasi-spherical focal spot in two-photon scanning microscopy by three-ring apodization

2005

International audience; We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread function gets a quasi-spherical shape. Such an improvement, which allows the acquisition of 3D images with isotropic quality, is obtained at the expense of only a small increase of the overall energy in the axial sidelobes. The performance of this technique is illustrated with a scanning TPE microscopy experiment in which the image of small beads is obtained. We demonstrat…

Fast multi-directional DSLM for confocal detection without striping artifacts

2020

In recent years light-sheet fluorescence microscopy (LSFM) has become a cornerstone technology for neuroscience, improving the quality and capabilities of 3D imaging. By selectively illuminating a single plane, it provides intrinsic optical sectioning and fast image recording, while minimizing out of focus fluorescence background, sample photo-damage and photo-bleaching. However, images acquired with LSFM are often affected by light absorption or scattering effects, leading to un-even illumination and striping artifacts. In this work we present an optical solution to this problem, via fast multi-directional illumination of the sample, based on an acousto-optical deflector (AOD). We demonstr…

Axial resolution in two-color excitation fluorescence microscopy by phase-only binary apodization

2005

We study the effect of a kind of binary phase-only filters, the Toraldo filters, in two-color excitation fluorescence microscopy. We show that by simple insertion of a properly designed Toraldo filter in one of the illumination arms the axial resolution of the system is significantly improved. Specifically, the main peak of the point spread function is narrowed by 22% along the axial direction.

Light Sheet Fluorescence Microscopy (LSFM) for Two-Photon Excitation Imaging of Thick Samples.

2015

Over the last decades, fluorescence microscopy techniques have been developed in order to provide a deeper, faster and higher resolution imaging of three-dimensional biological samples. Within this framework, Light Sheet Fluorescence Microscopy (LSFM) became an increasingly useful and popular imaging technique able to answer several biological questions in the field of developmental biology [1]. Thanks to the spatial confinement of the excitation process within a thin sheet in the focal plane, it provides an intrinsic optical sectioning and a reduced phototoxicity. On the other side, Two-Photon Excitation (2PE), thanks to the use of IR wavelengths, has become an invaluable tool to improve i…

Is Your Moss Alive during Active Biomonitoring Study?

2021

Biomonitoring was proposed to assess the condition of living organisms or entire ecosystems with the use of bioindicators—species sensitive to specific pollutants. It is important that the bioindicator species remains alive for as long as possible while retaining the ability to react to the negative effects of pollution (elimination/neutralization of hazardous contaminants). The purpose of the study was to assess the survival of Pleurozium schreberi moss during exposure (moss-bag technique) based on the measurement of the concentration of elements (Ni, Cu, Zn, Cd, and Pb), chlorophyll content, and its fluorescence. The study was carried out using a CCM-300 portable chlorophyll content meter…

Similar Effects of Ozone on Four Cultivars of Lettuce in Open Top Chambers During Winter

2002

Ozone is the major phytotoxic air pollutant that reduces the yield of several agricultural crops in the Spanish Mediterranean area. We studied four lettuce cultivars (Lactuca sativa L.) for the effects of different O3 concentrations during the winter on chlorophyll (Chl) a fluorescence, lipid peroxidation, and root length in outdoor open-top chambers. Under O3 the photosynthetic quantum conversion declined while heat emissions increased in all cultivars; these results provide more evidence of non-filtered air with additional ozone (NFA+O3) treatment compared with non-filtered air (NFA) and charcoal filtered ozone-free air (CFA). Changes in the Chl a fluorescence may be associated with an in…

Releasing rates of inorganic ions in lichens monitored by capillary zone electrophoresis as indicators of atmospheric pollution

2000

Abstract The releasing rates of inorganic ions in lichens when a thallus sample is sonicated under water, were studied. Ramalina farinacea samples taken from two locations exposed to atmospheric pollution sources at Els Ports (Castellon, Spain), and from three control locations, were used. Samples without visual symptoms of injuries were sonicated from 3 to 6 successive 10-min periods while renewing the water layer in between. Experiments with successive 2-min-sonication periods were also performed. The extracts were analysed by capillary zone electrophoresis, and K + , NH 4 + , Na + , Ca 2+ , Mg 2+ , Cl − , SO 4 2− and NO 3 − were detected. The apparently undamaged lichens sampled at locat…

The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation.

2008

ATP-dependent nucleosome-remodeling enzymes and covalent modifiers of chromatin set the functional state of chromatin. However, how these enzymatic activities are coordinated in the nucleus is largely unknown. We found that the evolutionary conserved nucleosome-remodeling ATPase ISWI and the poly-ADP-ribose polymerase PARP genetically interact. We present evidence showing that ISWI is target of poly-ADP-ribosylation. Poly-ADP-ribosylation counteracts ISWI function in vitro and in vivo. Our work suggests that ISWI is a physiological target of PARP and that poly-ADP-ribosylation can be a new, important post-translational modification regulating the activity of ATP-dependent nucleosome remodel…