Search results for "Fluorescence"
showing 10 items of 2463 documents
Rb atomu leņķiskā momenta šķērsorientācijas eksperimentāli pētījumi ārējā magnētiskajā laukā, ierosinot D1 līnijas supersīkstruktūras pārejas
2020
Šajā darbā tiek pētīta rubīdija leņķiskā momenta izkārtošanas pāreja šķērsorientācijā, ko rada Rb D_1 līnijas supersīkstruktūras līmeņu mijiedarbība ar gaismu magnētiskajā laukā. Šis efekts tika konstatēts eksperimentāli, mērot divu atsevišķu, pretēji cirkulāri polarizētu lāzera inducētās fluorescences komponenšu signālu starpības. Tika iegūtas šo signālu atkarības no lāzera frekvences un intensitātes. Iegūtie eksperimentālie dati tika salīdzināti ar teorētiskajiem, kas iegūti, izmantojot LU Lāzeru Centrā attīstītu teorētisko modeli. Papildus tam tika analizēti eksperimentālo signālu veidošanās un to formas cēloņi, kā arī to saistība ar supersīkstruktūras magnētisko apakšlīmeņu pāreju uzved…
Magnetooptisko signālu modēlēšana Cs atomu tvaikos, izmantojot "Julia" programmēšanas rīkus
2022
Tika izstrādāta jauna programma fluorescences un absorbcijas signālu modelēšanai, iekļaujot kodā signālu atkarību no ārējā magnētiskā lauka un Doplera efekta. Signāli tika modelēti, izmantojot optiskos Bloha vienādojumus, lai iegūtu blīvuma matricas, kas raksturo atomu kvantu stāvokļus. Lai modelētu signālus Cs D_1 līnijai, kodā tika iekļauta pumpējošā un zondējošā stara raksturojošie parametri. Šāda staru konfigurācija ir noderīga magnetometra izstrādes procesā. Tika apskatīti vairāki parametri magnetometra optimizācijai. Iegūtie aprēķini liecina, ka magnetometra izstrādei optimālākā supersīkstruktūras pāreja Cs D1 līnijā ir F_g=4→F_e=4, piemērotākā Rabi frekvence ir aptuveni 0.5 MHz, un i…
2019
Ultrasmall polyaminocarboxylate-coated gold nanoparticles (NPs), Au@DTDTPA and Au@TADOTAGA, that have been recently developed exhibit a promising potential for image-guided radiotherapy. In order to render the radiosensitizing effect of these gold nanoparticles even more efficient, the study of their localization in cells is required to better understand the relation between the radiosensitizing properties of the agents and their localization in cells and in tumors. To achieve this goal, post-functionalization of Au@DTDTPA nanoparticles by near-infrared (NIF) organic dyes (aminated derivative of cyanine 5, Cy5-NH2) was performed. The immobilization of organic Cy5-NH2 dyes onto the gold nano…
Micro-X-Ray Fluorescence and the Old Masters Non-destructive in situ characterisation of the varnish of historical Low Countries stringed musical ins…
2012
Applied Physics A, 107
Zein as biodegradable material for effective delivery of alkaline phosphatase and substrates in biokits and biosensors
2016
A biodegradable material, zein, is proposed as a reagent delivery platform for biokits and biosensors based on alkaline phosphatase (ALP) activity/inhibition in the presence of phosphatase substrates. The immobilization and release of both the substrate and/or the active ALP, in a biodegradable and low-cost material such as zein, a prolamin from maize, and in combination with glycerol as plasticizer have been investigated. Three zein-based devices are proposed for several applications: (1) inorganic phosphorus estimation in water of different sources (river, lake, coastal water and tap water) with a detection limit of 0.2mg/L - compared to at least 1mg/L required by legislation, (2) estimat…
Automated approach for indirect immunofluorescence images classification based on unsupervised clustering method
2018
Autoimmune diseases (ADs) are a collection of many complex disorders of unknown aetiology resulting in immune responses to self-antigens and are thought to result from interactions between genetic and environmental factors. ADs collectively are amongst the most prevalent diseases in the U.S., affecting at least 7% of the population. The diagnosis of ADs is very complex, the standard screening methods provides seeking and recognizing of Antinuclear Antibodies (ANA) by Indirect ImmunoFluorescence (IIF) based on HEp-2 cells. In this paper an automatic system able to identify and classify the Centromere pattern is presented. The method is based on the grouping of centromeres present on the cell…
Alginate encapsulation improves viability and integrity of cryopreserved pancreatic islets and multicellular spheroids: combined fluorescence, scanni…
2007
Extended abstract of a paper presented at MC 2007, 33rd DGE Conference in Saarbrücken, Germany, September 2 – September 7, 2007
Apparent content curves: new analytical applications
1993
Analytical possibilities of apparent content curves for ternary systems are studied. The mathematical method proposed is applied to UV-Vis spectrophotometric and fluorimetric techniques. Samples containing fluoresceine/eosine/acridine and methyl red/methyl orange/cresol red are resolved by fluorimetry and UV-spectroscopy, respectively. Results obtained are in agreement with the theoretical contents in both cases.
Fine Mapping of Gene Ordering by Elongated Chromosome Methods
2006
Publisher Summary Fluorescence in situ hybridization (FISH) can be used to localize specific DNA sequences on metaphase chromosomes, interphase nuclei, and experimentally extended DNA or chromatin fibers. Depending on the hybridization target, FISH techniques show widely different levels of DNA resolution. Mechanically stretched or elongated chromosomes fill the resolution gap between metaphase FISH and fiber FISH, allowing the rapid and straightforward ordering and localization of clones along the length of an entire chromosome with a 100- to 200-kb resolution. Although various genome projects have provided very high-resolution physical maps of human and important animal genomes, FISH is s…
Chromosomal assignment of the ovine hairless (hr) gene by fluorescence insitu hybridization
2008
Finocchiaro, F., Castiglioni, B., Budelli, E., van Kaam, J.B.C.H.M., Portolano, B., Caroli, A., Pagnacco, G. 2008.Chromosomal assignment of the ovine hairless (hr) gene by fluorescence in situ hybridization *Hereditas 145: 258 261.Lund, Sweden. eISSN 1601-5223. Received February 25, 2008. Accepted May 26, 2008E-mail: raffaellafinocchiaro@anafi.it